Using a supercontinuum light source for instantaneous excitation-emission fluorescence mapping

Johannes Kiefer*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fluorescent species are commonly utilized to visualize molecular and biological processes in the chemical and life sciences. A fast method for characterizing the excitation-wavelength-dependent emission spectrum is proposed based on imaging the fluorescence from the dispersed radiation of a supercontinuum light source to a spectrograph. The full excitation-emission map can be acquired within a fraction of a second and hence the possibility for rapid screening and the investigation of transient phenomena.

Original languageEnglish
Article number067001
Number of pages4
JournalMeasurement Science and Technology
Volume28
Issue number6
DOIs
Publication statusPublished - Jun 2017

Keywords

  • fluorescence spectroscopy
  • supercontinuum
  • excitation-emission matrix
  • DIFFERENCE SPECTROSCOPY ISERDS
  • MICROSCOPY

Cite this

Using a supercontinuum light source for instantaneous excitation-emission fluorescence mapping. / Kiefer, Johannes.

In: Measurement Science and Technology, Vol. 28, No. 6, 067001, 06.2017.

Research output: Contribution to journalArticle

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KW - excitation-emission matrix

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KW - MICROSCOPY

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