Abstract
Fluorescent species are commonly utilized to visualize molecular and biological processes in the chemical and life sciences. A fast method for characterizing the excitation-wavelength-dependent emission spectrum is proposed based on imaging the fluorescence from the dispersed radiation of a supercontinuum light source to a spectrograph. The full excitation-emission map can be acquired within a fraction of a second and hence the possibility for rapid screening and the investigation of transient phenomena.
Original language | English |
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Article number | 067001 |
Number of pages | 4 |
Journal | Measurement Science and Technology |
Volume | 28 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2017 |
Keywords
- fluorescence spectroscopy
- supercontinuum
- excitation-emission matrix
- DIFFERENCE SPECTROSCOPY ISERDS
- MICROSCOPY