Fluorescent species are commonly utilized to visualize molecular and biological processes in the chemical and life sciences. A fast method for characterizing the excitation-wavelength-dependent emission spectrum is proposed based on imaging the fluorescence from the dispersed radiation of a supercontinuum light source to a spectrograph. The full excitation-emission map can be acquired within a fraction of a second and hence the possibility for rapid screening and the investigation of transient phenomena.
- fluorescence spectroscopy
- excitation-emission matrix
- DIFFERENCE SPECTROSCOPY ISERDS