Utilization of Polyadenylated mRNA during Growth and Starvation in Physarum polycephalum

Alistair J.P. BROWN; Norman HARDMAN

doi:10.1111/j.1432-1033.1980.tb04882.x

Utilization of Polyadenylated mRNA during Growth and Starvation in Physarum polycephalum

Alistair J.P. BROWN^*, Norman HARDMAN

^*Corresponding author for this work

Medical Sciences

University of Aberdeen

Research output: Contribution to journal › Article › peer-review

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Abstract

The effect of growth on the efficiency of utilization of poly(A)‐containing mRNA for translation has been investigated in microplasmodia of Physarum polycephalum. Measurement of the relative proportions of poly(A)‐rich mRNA in polysomal and post‐polysomal fractions isolated by sucrose density gradient centrifugation reveals that newly synthesized poly(A)‐rich mRNA is present in increasing proportions in the polysomal region during exponential growth. However, the proportion of long‐lived poly(A)‐rich mRNA observed in actively‐translating polysomes declines as starvation approaches. The ribonuclease content and morphology of the microplasmodia were monitored during growth and starvation in an effort to relate this phenomenon to the onset of spherulation.

Original language	English
Pages (from-to)	413-420
Number of pages	8
Journal	European Journal of Biochemistry
Volume	110
Issue number	2
DOIs	https://doi.org/10.1111/j.1432-1033.1980.tb04882.x
Publication status	Published - 1 Jan 1980

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10.1111/j.1432-1033.1980.tb04882.x

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title = "Utilization of Polyadenylated mRNA during Growth and Starvation in Physarum polycephalum",

abstract = "The effect of growth on the efficiency of utilization of poly(A)‐containing mRNA for translation has been investigated in microplasmodia of Physarum polycephalum. Measurement of the relative proportions of poly(A)‐rich mRNA in polysomal and post‐polysomal fractions isolated by sucrose density gradient centrifugation reveals that newly synthesized poly(A)‐rich mRNA is present in increasing proportions in the polysomal region during exponential growth. However, the proportion of long‐lived poly(A)‐rich mRNA observed in actively‐translating polysomes declines as starvation approaches. The ribonuclease content and morphology of the microplasmodia were monitored during growth and starvation in an effort to relate this phenomenon to the onset of spherulation.",

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AU - HARDMAN, Norman

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N2 - The effect of growth on the efficiency of utilization of poly(A)‐containing mRNA for translation has been investigated in microplasmodia of Physarum polycephalum. Measurement of the relative proportions of poly(A)‐rich mRNA in polysomal and post‐polysomal fractions isolated by sucrose density gradient centrifugation reveals that newly synthesized poly(A)‐rich mRNA is present in increasing proportions in the polysomal region during exponential growth. However, the proportion of long‐lived poly(A)‐rich mRNA observed in actively‐translating polysomes declines as starvation approaches. The ribonuclease content and morphology of the microplasmodia were monitored during growth and starvation in an effort to relate this phenomenon to the onset of spherulation.

AB - The effect of growth on the efficiency of utilization of poly(A)‐containing mRNA for translation has been investigated in microplasmodia of Physarum polycephalum. Measurement of the relative proportions of poly(A)‐rich mRNA in polysomal and post‐polysomal fractions isolated by sucrose density gradient centrifugation reveals that newly synthesized poly(A)‐rich mRNA is present in increasing proportions in the polysomal region during exponential growth. However, the proportion of long‐lived poly(A)‐rich mRNA observed in actively‐translating polysomes declines as starvation approaches. The ribonuclease content and morphology of the microplasmodia were monitored during growth and starvation in an effort to relate this phenomenon to the onset of spherulation.

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Utilization of Polyadenylated mRNA during Growth and Starvation in Physarum polycephalum

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