Vaccination of pigs with the S48 strain of Toxoplasma gondii

safer meat for human consumption

Alison Burrells, Julio Benavides, German Cantón, João L. Garcia, Paul M. Bartley, Mintu Nath, Jackie Thomson, Francesca Chianini, Elisabeth A. Innes, Frank Katzer* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination (incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results from the mouse bioassay show that 100% of mice which had received porcine tissues from vaccinated and challenged pigs survived compared with 51.1% of mice which received tissues from non-vaccinated and challenged pigs. The presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48 strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus improving food safety.

Original languageEnglish
Article number47
Number of pages12
JournalVeterinary Research
Volume46
Issue number1
DOIs
Publication statusPublished - 1 May 2015

Fingerprint

Toxoplasma
Toxoplasma gondii
Meat
Vaccination
Swine
vaccination
meat
swine
Cysts
mice
pork
Biological Assay
loins (meat cut)
Parasites
bioassays
human food chain
meat cuts
brain
tissues
parasites

Keywords

  • mouse brain
  • tissue cyst
  • gold standard test
  • porcine tissue
  • mouse bioassay

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Burrells, A., Benavides, J., Cantón, G., Garcia, J. L., Bartley, P. M., Nath, M., ... Katzer, F. (2015). Vaccination of pigs with the S48 strain of Toxoplasma gondii: safer meat for human consumption. Veterinary Research, 46(1), [47]. https://doi.org/10.1186/s13567-015-0177-0

Vaccination of pigs with the S48 strain of Toxoplasma gondii : safer meat for human consumption. / Burrells, Alison; Benavides, Julio; Cantón, German; Garcia, João L.; Bartley, Paul M.; Nath, Mintu; Thomson, Jackie; Chianini, Francesca; Innes, Elisabeth A.; Katzer, Frank (Corresponding Author).

In: Veterinary Research, Vol. 46, No. 1, 47, 01.05.2015.

Research output: Contribution to journalArticle

Burrells, A, Benavides, J, Cantón, G, Garcia, JL, Bartley, PM, Nath, M, Thomson, J, Chianini, F, Innes, EA & Katzer, F 2015, 'Vaccination of pigs with the S48 strain of Toxoplasma gondii: safer meat for human consumption', Veterinary Research, vol. 46, no. 1, 47. https://doi.org/10.1186/s13567-015-0177-0
Burrells, Alison ; Benavides, Julio ; Cantón, German ; Garcia, João L. ; Bartley, Paul M. ; Nath, Mintu ; Thomson, Jackie ; Chianini, Francesca ; Innes, Elisabeth A. ; Katzer, Frank. / Vaccination of pigs with the S48 strain of Toxoplasma gondii : safer meat for human consumption. In: Veterinary Research. 2015 ; Vol. 46, No. 1.
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abstract = "As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination (incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results from the mouse bioassay show that 100{\%} of mice which had received porcine tissues from vaccinated and challenged pigs survived compared with 51.1{\%} of mice which received tissues from non-vaccinated and challenged pigs. The presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48 strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus improving food safety.",
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