TY - JOUR
T1 - Validation and inter-laboratory study of selective hydride generation for fast screening of inorganic arsenic in seafood
AU - Marschner, Karel
AU - Pétursdóttir, Ásta H.
AU - Bücker, Patrick
AU - Raab, Andrea
AU - Feldmann, Jörg
AU - Mester, Zoltán
AU - Matoušek, Tomáš
AU - Musil, Stanislav
N1 - This work was supported by Institute of Analytical Chemistry of the Czech Academy of Sciences (project no. RVO: 68081715) and by Ministry of Education, Youth and Sports of the CR (project Kontakt II LH15174). This project has received funding from the European Union´s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 656596 (Dr. Ásta H. Pétursdóttir). Patrick Bücker thanks the EU Erasmus programme for financial support.
PY - 2019/2/21
Y1 - 2019/2/21
N2 - It is advisable to monitor and regulate inorganic arsenic (iAs) in food and feedstuff. This work describes an update and validation of a method of selective hydride generation (HG) with inductively coupled plasma mass spectrometry (ICP-MS) for high-throughput screening of iAs content in seafood samples after microwave-assisted extraction with diluted nitric acid and hydrogen peroxide. High concentration of HCl (8 M) for HG along with hydrogen peroxide in samples of a same concentration as used for extraction leads to a selective conversion of iAs to volatile arsine that is released and transported to the detector. A minor contribution from methylarsonate (≈20% to iAs) was found, while HG from dimethylarsinate, trimethylarsine oxide is substantially suppressed (less than 1% to iAs). Methodology was applied to Certified Reference Materials (CRMs) TORT-3, DORM-3, DORM-4, DOLT-4, DOLT-5, PRON-1, SQID-1 and ERM-CE278k, in some of them iAs has been determined for the first time, and to various seaweed samples from a local store. The results were always compared with a reference method and selectivity of iAs determination was evaluated. An inter-laboratory reproducibility was tested by comparative analyses of six fish and four seaweed samples in three European laboratories, with good agreement of the results. The method of HG-ICP-MS is sensitive (limit of detection 2 μg kg−1 iAs), well suited for screening of large number of samples and selective at iAs concentration levels at which maximum limits are expected to be set into EU legislation for marine samples.
AB - It is advisable to monitor and regulate inorganic arsenic (iAs) in food and feedstuff. This work describes an update and validation of a method of selective hydride generation (HG) with inductively coupled plasma mass spectrometry (ICP-MS) for high-throughput screening of iAs content in seafood samples after microwave-assisted extraction with diluted nitric acid and hydrogen peroxide. High concentration of HCl (8 M) for HG along with hydrogen peroxide in samples of a same concentration as used for extraction leads to a selective conversion of iAs to volatile arsine that is released and transported to the detector. A minor contribution from methylarsonate (≈20% to iAs) was found, while HG from dimethylarsinate, trimethylarsine oxide is substantially suppressed (less than 1% to iAs). Methodology was applied to Certified Reference Materials (CRMs) TORT-3, DORM-3, DORM-4, DOLT-4, DOLT-5, PRON-1, SQID-1 and ERM-CE278k, in some of them iAs has been determined for the first time, and to various seaweed samples from a local store. The results were always compared with a reference method and selectivity of iAs determination was evaluated. An inter-laboratory reproducibility was tested by comparative analyses of six fish and four seaweed samples in three European laboratories, with good agreement of the results. The method of HG-ICP-MS is sensitive (limit of detection 2 μg kg−1 iAs), well suited for screening of large number of samples and selective at iAs concentration levels at which maximum limits are expected to be set into EU legislation for marine samples.
KW - Certified reference materials
KW - Inductively coupled plasma mass spectrometry
KW - Inorganic arsenic
KW - Interlaboratory comparison
KW - Selective hydride generation
KW - Speciation analysis
UR - http://www.scopus.com/inward/record.url?scp=85057229279&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2018.11.036
DO - 10.1016/j.aca.2018.11.036
M3 - Article
AN - SCOPUS:85057229279
VL - 1049
SP - 20
EP - 28
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
ER -
