Validation of a PCR-RFLP based method for the identification of salmon species in food products.

Georgina Louise Hold, Valerie Joan Russell, S. E. Pryde, H. Rehbein, J. Quinterio, M. Rey-Mendez, C. G. Sotelo, R. I. Perez-Martin, A. Santos, C. Rosa

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

The use of a PCR-RFLP based method for the identification of salmon species in food products was investigated. The reliability and practicality of the method was tested by a collaborative study in which five European laboratories participated. Two unknown samples (a commercial product of known species composition and a mix of two salmon species) required identification by comparison with authentic reference species. From a total of 50 cases, 100% of authentic species were correctly assigned, with all unknown samples also correctly identified. A larger scale analysis of UK commercial products was also performed spanning the whole range of salmon products available. In almost all cases the salmon species declared was confirmed, although, a trout species was detected in one product declaring only the presence of salmon. The investigation confirms the reproducibility of the method in different laboratories, and its applicability for commercial product analysis.

Original languageEnglish
Pages (from-to)385-389
Number of pages5
JournalEuropean Food Research and Technology
Volume212
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • salmon
  • species identification
  • PCR-RFLP
  • Inter-laboratory study

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