Validation of a PCR-RFLP based method for the identification of salmon species in food products.

Georgina Louise Hold; Valerie Joan Russell; S. E. Pryde; H. Rehbein; J. Quinterio; M. Rey-Mendez; C. G. Sotelo; R. I. Perez-Martin; A. Santos; C. Rosa

doi:10.1007/s002170000237

Validation of a PCR-RFLP based method for the identification of salmon species in food products.

Georgina Louise Hold, Valerie Joan Russell, S. E. Pryde, H. Rehbein, J. Quinterio, M. Rey-Mendez, C. G. Sotelo, R. I. Perez-Martin, A. Santos, C. Rosa

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Abstract

The use of a PCR-RFLP based method for the identification of salmon species in food products was investigated. The reliability and practicality of the method was tested by a collaborative study in which five European laboratories participated. Two unknown samples (a commercial product of known species composition and a mix of two salmon species) required identification by comparison with authentic reference species. From a total of 50 cases, 100% of authentic species were correctly assigned, with all unknown samples also correctly identified. A larger scale analysis of UK commercial products was also performed spanning the whole range of salmon products available. In almost all cases the salmon species declared was confirmed, although, a trout species was detected in one product declaring only the presence of salmon. The investigation confirms the reproducibility of the method in different laboratories, and its applicability for commercial product analysis.

Original language	English
Pages (from-to)	385-389
Number of pages	5
Journal	European Food Research and Technology
Volume	212
Issue number	3
DOIs	https://doi.org/10.1007/s002170000237
Publication status	Published - 2001

Keywords

salmon
species identification
PCR-RFLP
Inter-laboratory study

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10.1007/s002170000237

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title = "Validation of a PCR-RFLP based method for the identification of salmon species in food products.",

abstract = "The use of a PCR-RFLP based method for the identification of salmon species in food products was investigated. The reliability and practicality of the method was tested by a collaborative study in which five European laboratories participated. Two unknown samples (a commercial product of known species composition and a mix of two salmon species) required identification by comparison with authentic reference species. From a total of 50 cases, 100% of authentic species were correctly assigned, with all unknown samples also correctly identified. A larger scale analysis of UK commercial products was also performed spanning the whole range of salmon products available. In almost all cases the salmon species declared was confirmed, although, a trout species was detected in one product declaring only the presence of salmon. The investigation confirms the reproducibility of the method in different laboratories, and its applicability for commercial product analysis. ",

keywords = "salmon, species identification, PCR-RFLP, Inter-laboratory study",

author = "Hold, {Georgina Louise} and Russell, {Valerie Joan} and Pryde, {S. E.} and H. Rehbein and J. Quinterio and M. Rey-Mendez and Sotelo, {C. G.} and Perez-Martin, {R. I.} and A. Santos and C. Rosa",

year = "2001",

doi = "10.1007/s002170000237",

language = "English",

volume = "212",

pages = "385--389",

journal = "European Food Research and Technology",

issn = "1438-2377",

publisher = "Springer Verlag",

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T1 - Validation of a PCR-RFLP based method for the identification of salmon species in food products.

AU - Hold, Georgina Louise

AU - Russell, Valerie Joan

AU - Pryde, S. E.

AU - Rehbein, H.

AU - Quinterio, J.

AU - Rey-Mendez, M.

AU - Sotelo, C. G.

AU - Perez-Martin, R. I.

AU - Santos, A.

AU - Rosa, C.

PY - 2001

Y1 - 2001

N2 - The use of a PCR-RFLP based method for the identification of salmon species in food products was investigated. The reliability and practicality of the method was tested by a collaborative study in which five European laboratories participated. Two unknown samples (a commercial product of known species composition and a mix of two salmon species) required identification by comparison with authentic reference species. From a total of 50 cases, 100% of authentic species were correctly assigned, with all unknown samples also correctly identified. A larger scale analysis of UK commercial products was also performed spanning the whole range of salmon products available. In almost all cases the salmon species declared was confirmed, although, a trout species was detected in one product declaring only the presence of salmon. The investigation confirms the reproducibility of the method in different laboratories, and its applicability for commercial product analysis.

AB - The use of a PCR-RFLP based method for the identification of salmon species in food products was investigated. The reliability and practicality of the method was tested by a collaborative study in which five European laboratories participated. Two unknown samples (a commercial product of known species composition and a mix of two salmon species) required identification by comparison with authentic reference species. From a total of 50 cases, 100% of authentic species were correctly assigned, with all unknown samples also correctly identified. A larger scale analysis of UK commercial products was also performed spanning the whole range of salmon products available. In almost all cases the salmon species declared was confirmed, although, a trout species was detected in one product declaring only the presence of salmon. The investigation confirms the reproducibility of the method in different laboratories, and its applicability for commercial product analysis.

KW - salmon

KW - species identification

KW - PCR-RFLP

KW - Inter-laboratory study

U2 - 10.1007/s002170000237

DO - 10.1007/s002170000237

M3 - Article

SN - 1438-2377

VL - 212

SP - 385

EP - 389

JO - European Food Research and Technology

JF - European Food Research and Technology

IS - 3

ER -

Validation of a PCR-RFLP based method for the identification of salmon species in food products.

Abstract

Keywords

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