Abstract
When the doubly-labeled water (DLW) method is used to measure total daily energy expenditure (TDEE), isotope measurements are typically performed using isotope ratio mass spectrometry (IRMS). New technologies, such as off-axis integrated cavity output spectroscopy (OA-ICOS) provide comparable isotopic measurements of standard waters and human urine samples, but the accuracy of carbon dioxide production (VCO2) determined with OA-ICOS has not been demonstrated. We compared simultaneous measurement of VCO2 obtained using whole-room indirect calorimetry (IC) with DLW-based measurements from IRMS and OA-ICOS. 17 subjects (10 female; 22 to 63 yrs.) were studied for 7 consecutive days in the IC. Subjects consumed a dose of 0.25 g H2(18)O (95% APE) and 0.14 g (2)H2O (99.8% APE) per kg of total body water, and urine samples were obtained on days 1 and 8 to measure average daily VCO2 using OA-ICOS and IRMS. VCO2 was calculated using both the plateau and intercept methods. There were no differences in VCO2 or TDEE measured by OA-ICOS or IRMS compared with IC when the plateau method was used. When the intercept method was used, VCO2 measured using OA-ICOS did not differ from IC, but VCO2 measured using IRMS was significantly lower than IC. Accuracy (~1-5%), precision (~8%), intraclass correlation coefficients (R=0.87-90), and root mean squared error (30-40 L/day) of VCO22 measured by OA-ICOS and IRMS were similar. Both OA-ICOS and IRMS produced measurements of VCO2 with comparable accuracy and precision when compared to IC.
| Original language | English |
|---|---|
| Pages (from-to) | 124-130 |
| Number of pages | 7 |
| Journal | American Journal of Physiology: Endocrinology and Metabolism |
| Volume | 314 |
| Issue number | 2 |
| Early online date | 3 Oct 2017 |
| DOIs | |
| Publication status | Published - Feb 2018 |
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Keywords
- Journal Article
- Adult
- Humans
- Oxygen Isotope
- Deuterium
- Respiratory Gas Exchange
Cite this
Validation of the doubly labeled water method using off-axis integrated cavity output spectroscopy and isotope ratio mass spectrometry. / Melanson, Edward L.; Swibas, Tracy; Kohrt, Wendy M.; Catenacci, Vicki A.; Creasy, Seth A.; Plasqui, Guy; Wouters, Loek; Speakman, John R; Berman, Elena S. F.
In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 314, No. 2, 02.2018, p. 124-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Validation of the doubly labeled water method using off-axis integrated cavity output spectroscopy and isotope ratio mass spectrometry
AU - Melanson, Edward L.
AU - Swibas, Tracy
AU - Kohrt, Wendy M.
AU - Catenacci, Vicki A.
AU - Creasy, Seth A.
AU - Plasqui, Guy
AU - Wouters, Loek
AU - Speakman, John R
AU - Berman, Elena S. F.
N1 - This work was supported by an NIH Small Business Innovation (SBIR) research Grant (R44 DK093362), as well as support from the Colorado Nutrition and Obesity Research Center (P30 DK048520) and the Colorado Clinical and Translational Science Institute (UL1 RR025780). Dr. Melanson is also supported by resources from the Geriatric Research, Education, and Clinical Center at the Denver VA Medical Center.
PY - 2018/2
Y1 - 2018/2
N2 - When the doubly-labeled water (DLW) method is used to measure total daily energy expenditure (TDEE), isotope measurements are typically performed using isotope ratio mass spectrometry (IRMS). New technologies, such as off-axis integrated cavity output spectroscopy (OA-ICOS) provide comparable isotopic measurements of standard waters and human urine samples, but the accuracy of carbon dioxide production (VCO2) determined with OA-ICOS has not been demonstrated. We compared simultaneous measurement of VCO2 obtained using whole-room indirect calorimetry (IC) with DLW-based measurements from IRMS and OA-ICOS. 17 subjects (10 female; 22 to 63 yrs.) were studied for 7 consecutive days in the IC. Subjects consumed a dose of 0.25 g H2(18)O (95% APE) and 0.14 g (2)H2O (99.8% APE) per kg of total body water, and urine samples were obtained on days 1 and 8 to measure average daily VCO2 using OA-ICOS and IRMS. VCO2 was calculated using both the plateau and intercept methods. There were no differences in VCO2 or TDEE measured by OA-ICOS or IRMS compared with IC when the plateau method was used. When the intercept method was used, VCO2 measured using OA-ICOS did not differ from IC, but VCO2 measured using IRMS was significantly lower than IC. Accuracy (~1-5%), precision (~8%), intraclass correlation coefficients (R=0.87-90), and root mean squared error (30-40 L/day) of VCO22 measured by OA-ICOS and IRMS were similar. Both OA-ICOS and IRMS produced measurements of VCO2 with comparable accuracy and precision when compared to IC.
AB - When the doubly-labeled water (DLW) method is used to measure total daily energy expenditure (TDEE), isotope measurements are typically performed using isotope ratio mass spectrometry (IRMS). New technologies, such as off-axis integrated cavity output spectroscopy (OA-ICOS) provide comparable isotopic measurements of standard waters and human urine samples, but the accuracy of carbon dioxide production (VCO2) determined with OA-ICOS has not been demonstrated. We compared simultaneous measurement of VCO2 obtained using whole-room indirect calorimetry (IC) with DLW-based measurements from IRMS and OA-ICOS. 17 subjects (10 female; 22 to 63 yrs.) were studied for 7 consecutive days in the IC. Subjects consumed a dose of 0.25 g H2(18)O (95% APE) and 0.14 g (2)H2O (99.8% APE) per kg of total body water, and urine samples were obtained on days 1 and 8 to measure average daily VCO2 using OA-ICOS and IRMS. VCO2 was calculated using both the plateau and intercept methods. There were no differences in VCO2 or TDEE measured by OA-ICOS or IRMS compared with IC when the plateau method was used. When the intercept method was used, VCO2 measured using OA-ICOS did not differ from IC, but VCO2 measured using IRMS was significantly lower than IC. Accuracy (~1-5%), precision (~8%), intraclass correlation coefficients (R=0.87-90), and root mean squared error (30-40 L/day) of VCO22 measured by OA-ICOS and IRMS were similar. Both OA-ICOS and IRMS produced measurements of VCO2 with comparable accuracy and precision when compared to IC.
KW - Journal Article
KW - Adult
KW - Humans
KW - Oxygen Isotope
KW - Deuterium
KW - Respiratory Gas Exchange
U2 - 10.1152/ajpendo.00241.2017
DO - 10.1152/ajpendo.00241.2017
M3 - Article
C2 - 28978547
VL - 314
SP - 124
EP - 130
JO - American Journal of Physiology: Endocrinology and Metabolism
JF - American Journal of Physiology: Endocrinology and Metabolism
SN - 0193-1849
IS - 2
ER -