Vanilloid receptor type 1-immunoreactivity is expressed by intrinsic afferent neurones in the guinea-pig myenteric plexus

Sharon Anavi-Goffer, N. G. McKay, M. L. Ashford, Angela Alice Coutts

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The enteric sensory nervous system consists of extrinsic and intrinsic components. The cellular distribution of vanilloid receptor type 1 (VR1) and its relation to the intrinsic sensory neurones were studied in myenteric plexus-longitudinal muscle preparations of rat ileum and guinea-pig ileum and colon. VR1-immunoreactivity was localized on fine fibres and expressed by ganglionic cells. In the guinea-pig myenteric plexus, a proportion of VR1-immunoreactive cells co-localized with calbindin, a marker for intrinsic afferent neurones. Reverse transcription-polymerase chain reaction with rat VR1-specific primers detected VR1 mRNA in rat but not in guinea-pig preparations. We conclude that VR1 is expressed on fibres and by myenteric neurones. In the guinea-pig, VR1 is expressed by intrinsic afferent neurones but its mRNA may differ from the rat sequence in the region of the primers. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)53-57
Number of pages4
JournalNeuroscience Letters
Volume319
Issue number1
DOIs
Publication statusPublished - 2002

Keywords

  • sensory neurones
  • calbindin
  • ileum
  • colon
  • rat
  • reverse transcription-polymerase chain reaction
  • SENSORY NEURONS
  • NEUROCHEMICAL CLASSIFICATION
  • CAPSAICIN
  • ILEUM
  • NERVES
  • VR1
  • RAT

Cite this

Vanilloid receptor type 1-immunoreactivity is expressed by intrinsic afferent neurones in the guinea-pig myenteric plexus. / Anavi-Goffer, Sharon; McKay, N. G.; Ashford, M. L.; Coutts, Angela Alice.

In: Neuroscience Letters, Vol. 319, No. 1, 2002, p. 53-57.

Research output: Contribution to journalArticle

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abstract = "The enteric sensory nervous system consists of extrinsic and intrinsic components. The cellular distribution of vanilloid receptor type 1 (VR1) and its relation to the intrinsic sensory neurones were studied in myenteric plexus-longitudinal muscle preparations of rat ileum and guinea-pig ileum and colon. VR1-immunoreactivity was localized on fine fibres and expressed by ganglionic cells. In the guinea-pig myenteric plexus, a proportion of VR1-immunoreactive cells co-localized with calbindin, a marker for intrinsic afferent neurones. Reverse transcription-polymerase chain reaction with rat VR1-specific primers detected VR1 mRNA in rat but not in guinea-pig preparations. We conclude that VR1 is expressed on fibres and by myenteric neurones. In the guinea-pig, VR1 is expressed by intrinsic afferent neurones but its mRNA may differ from the rat sequence in the region of the primers. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.",
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AB - The enteric sensory nervous system consists of extrinsic and intrinsic components. The cellular distribution of vanilloid receptor type 1 (VR1) and its relation to the intrinsic sensory neurones were studied in myenteric plexus-longitudinal muscle preparations of rat ileum and guinea-pig ileum and colon. VR1-immunoreactivity was localized on fine fibres and expressed by ganglionic cells. In the guinea-pig myenteric plexus, a proportion of VR1-immunoreactive cells co-localized with calbindin, a marker for intrinsic afferent neurones. Reverse transcription-polymerase chain reaction with rat VR1-specific primers detected VR1 mRNA in rat but not in guinea-pig preparations. We conclude that VR1 is expressed on fibres and by myenteric neurones. In the guinea-pig, VR1 is expressed by intrinsic afferent neurones but its mRNA may differ from the rat sequence in the region of the primers. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

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