Variability in E-selectin expression, mRNA levels and sE-selectin release between endothelial cell lines and primary endothelial cells

H F Galley, M G Blaylock, A M Dubbels, N R Webster

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Endothelial cell lines express markers and are assumed to exhibit other endothelial cell responses. We investigated E-selectin expression from human umbilical vein endothelial cells, the spontaneously transformed ECV304 line and the hybrid line EA.hy926 by flow cytometry and immunofluorescence, mRNA and soluble E-selectin release. In cells exposed to tumour necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta), median (range) percentage of E-selectin-positive HUVECs increased from 1.6(0.9-6.2)% to 91.4(83.0-96.1)%, (P=0.001) using flow cytometry. In contrast, E-selectin expression by ECV304 and EA.hy926 cell lines was 100-fold lower. E-selectin mRNA was detectable after 2 h, maximal at 6 h in HUVECs and undetectable in EA.hy926 and ECV304 cell lines after exposure to TNF-alpha/IL-1 beta. sE-selectin accumulation increased (P=0.004) in HUVECs only. Neutrophil adherence to ECV304 and EA.hy926 cells was poor compared to HUVECs (P=0.004). The cell lines ECV304 and EA.hy926 do not exhibit normal endothelium expression of E-selectin, and may not be appropriate for studies of adhesion. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)91-99
Number of pages9
JournalCell Biology International
Volume24
Publication statusPublished - 2000

Keywords

  • endothelial cells
  • E-selectin
  • adhesion molecule
  • leucocyte
  • TUMOR-NECROSIS-FACTOR
  • VONWILLEBRAND-FACTOR
  • ADHESION MOLECULE-1
  • IN-VITRO
  • EA.HY926
  • ANTIGEN
  • NEUTROPHILS
  • SURFACE
  • ENZYME
  • VCAM-1

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