Variation in molybdenum content across broadly distributed populations of Arabidopsis thaliana is controlled by a mitochondrial molybdenum transporter (MOT1)

Ivan Baxter, Balasubramaniam Muthukumar, Hyeong Cheol Park, Peter Buchner, Brett Lahner, John Danku, Keyan Zhao, Joohyun Lee, Malcolm J Hawkesford, Mary Lou Guerinot, David E Salt

Research output: Contribution to journalArticle

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Abstract

Molybdenum (Mo) is an essential micronutrient for plants, serving as a cofactor for enzymes involved in nitrate assimilation, sulfite detoxification, abscisic acid biosynthesis, and purine degradation. Here we show that natural variation in shoot Mo content across 92 Arabidopsis thaliana accessions is controlled by variation in a mitochondrially localized transporter (Molybdenum Transporter 1-MOT1) that belongs to the sulfate transporter superfamily. A deletion in the MOT1 promoter is strongly associated with low shoot Mo, occurring in seven of the accessions with the lowest shoot content of Mo. Consistent with the low Mo phenotype, MOT1 expression in low Mo accessions is reduced. Reciprocal grafting experiments demonstrate that the roots of Ler-0 are responsible for the low Mo accumulation in shoot, and GUS localization demonstrates that MOT1 is expressed strongly in the roots. MOT1 contains an N-terminal mitochondrial targeting sequence and expression of MOT1 tagged with GFP in protoplasts and transgenic plants, establishing the mitochondrial localization of this protein. Furthermore, expression of MOT1 specifically enhances Mo accumulation in yeast by 5-fold, consistent with MOT1 functioning as a molybdate transporter. This work provides the first molecular insight into the processes that regulate Mo accumulation in plants and shows that novel loci can be detected by association mapping.

Original languageEnglish
Article numbere1000004
Number of pages12
JournalPLoS Genetics
Volume4
Issue number2
DOIs
Publication statusPublished - 29 Feb 2008

Keywords

  • affinity molybdate transporter
  • binary vector
  • tomato plants
  • identification
  • polymorphisms
  • expression
  • proteins
  • cofactor
  • sequence
  • markers

Cite this

Variation in molybdenum content across broadly distributed populations of Arabidopsis thaliana is controlled by a mitochondrial molybdenum transporter (MOT1). / Baxter, Ivan; Muthukumar, Balasubramaniam; Park, Hyeong Cheol; Buchner, Peter; Lahner, Brett; Danku, John; Zhao, Keyan; Lee, Joohyun; Hawkesford, Malcolm J; Guerinot, Mary Lou; Salt, David E.

In: PLoS Genetics, Vol. 4, No. 2, e1000004, 29.02.2008.

Research output: Contribution to journalArticle

Baxter, I, Muthukumar, B, Park, HC, Buchner, P, Lahner, B, Danku, J, Zhao, K, Lee, J, Hawkesford, MJ, Guerinot, ML & Salt, DE 2008, 'Variation in molybdenum content across broadly distributed populations of Arabidopsis thaliana is controlled by a mitochondrial molybdenum transporter (MOT1)', PLoS Genetics, vol. 4, no. 2, e1000004. https://doi.org/10.1371/journal.pgen.1000004
Baxter, Ivan ; Muthukumar, Balasubramaniam ; Park, Hyeong Cheol ; Buchner, Peter ; Lahner, Brett ; Danku, John ; Zhao, Keyan ; Lee, Joohyun ; Hawkesford, Malcolm J ; Guerinot, Mary Lou ; Salt, David E. / Variation in molybdenum content across broadly distributed populations of Arabidopsis thaliana is controlled by a mitochondrial molybdenum transporter (MOT1). In: PLoS Genetics. 2008 ; Vol. 4, No. 2.
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