Vascular recruitment in skeletal muscle during exercise and hyperinsulinemia assessed by contrast ultrasound

Dana Dawson, Michelle A Vincent, Eugene J Barrett, Sanjiv Kaul, Andrew Clark, Howard Leong-Poi, Jonathan R Lindner

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to noninvasively quantify the effects of insulin on capillary blood volume (capBV) and RBC velocity (V(RBC)) in skeletal muscle in vivo with the use of contrast-enhanced ultrasound. We performed contrast ultrasound of the rat hindlimb adductor muscles at baseline and after 2-h infusions of either insulin (3 or 40 mU x kg(-1) x min(-1)) or saline. Saline-treated animals were also studied during contractile exercise. V(RBC) and capBV were calculated from the relation between pulsing interval and video intensity. Femoral artery blood flow, measured by a flow probe, increased with both contractile exercise and insulin. Contractile exercise increased capBV more than twofold and V(RBC) fivefold. Insulin also increased capBV more than twofold in a dose-dependent fashion but did not significantly alter V(RBC). Saline infusion did not significantly alter capBV, V(RBC), or femoral artery blood flow. We conclude that physiological changes in skeletal muscle capillary perfusion can be assessed in vivo with the use of contrast-enhanced ultrasound. Exercise increases both V(RBC) and capBV, whereas hyperinsulinemia selectively increases only capBV, which may enhance skeletal muscle glucose uptake.
Original languageEnglish
Pages (from-to)E714-20
JournalAmerican Journal of Physiology: Endocrinology and Metabolism
Volume282
Issue number3
DOIs
Publication statusPublished - 2002

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Hyperinsulinism
Blood Vessels
Blood Volume
Skeletal Muscle
Insulin
Femoral Artery
Hindlimb
Perfusion
Glucose
Muscles

Keywords

  • Animals
  • Blood Flow Velocity
  • Blood Volume
  • Capillaries
  • Erythrocytes
  • Femoral Artery
  • Glucose
  • Hyperinsulinism
  • Insulin
  • Male
  • Muscle Contraction
  • Muscle, Skeletal
  • Physical Exertion
  • Rats
  • Rats, Sprague-Dawley

Cite this

Vascular recruitment in skeletal muscle during exercise and hyperinsulinemia assessed by contrast ultrasound. / Dawson, Dana; Vincent, Michelle A; Barrett, Eugene J; Kaul, Sanjiv; Clark, Andrew; Leong-Poi, Howard; Lindner, Jonathan R.

In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 282, No. 3, 2002, p. E714-20.

Research output: Contribution to journalArticle

Dawson, Dana ; Vincent, Michelle A ; Barrett, Eugene J ; Kaul, Sanjiv ; Clark, Andrew ; Leong-Poi, Howard ; Lindner, Jonathan R. / Vascular recruitment in skeletal muscle during exercise and hyperinsulinemia assessed by contrast ultrasound. In: American Journal of Physiology: Endocrinology and Metabolism. 2002 ; Vol. 282, No. 3. pp. E714-20.
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AB - The purpose of this study was to noninvasively quantify the effects of insulin on capillary blood volume (capBV) and RBC velocity (V(RBC)) in skeletal muscle in vivo with the use of contrast-enhanced ultrasound. We performed contrast ultrasound of the rat hindlimb adductor muscles at baseline and after 2-h infusions of either insulin (3 or 40 mU x kg(-1) x min(-1)) or saline. Saline-treated animals were also studied during contractile exercise. V(RBC) and capBV were calculated from the relation between pulsing interval and video intensity. Femoral artery blood flow, measured by a flow probe, increased with both contractile exercise and insulin. Contractile exercise increased capBV more than twofold and V(RBC) fivefold. Insulin also increased capBV more than twofold in a dose-dependent fashion but did not significantly alter V(RBC). Saline infusion did not significantly alter capBV, V(RBC), or femoral artery blood flow. We conclude that physiological changes in skeletal muscle capillary perfusion can be assessed in vivo with the use of contrast-enhanced ultrasound. Exercise increases both V(RBC) and capBV, whereas hyperinsulinemia selectively increases only capBV, which may enhance skeletal muscle glucose uptake.

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