VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain

Miguel Tillo, Lynda Erskine, Anna Cariboni, Alessandro Fantin, Andy Joyce, Laura Denti, Christiana Ruhrberg

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Abstract

The vascular endothelial growth factor (VEGFA, VEGF) regulates neurovascular patterning. Alternative splicing of the Vegfa gene gives rise to three major isoforms termed VEGF121, VEGF165 and VEGF189. VEGF165 binds the transmembrane protein neuropilin 1 (NRP1) and promotes the migration, survival and axon guidance of subsets of neurons, whereas VEGF121 cannot activate NRP1-dependent neuronal responses. By contrast, the role of VEGF189 in NRP1-mediated signalling pathways has not yet been examined. Here, we have combined expression studies and in situ ligand-binding assays with the analysis of genetically altered mice and in vitro models to demonstrate that VEGF189 can bind NRP1 and promote NRP1-dependent neuronal responses.

Original languageEnglish
Pages (from-to)314-319
Number of pages6
JournalDevelopment
Volume142
Issue number2
Early online date17 Dec 2014
DOIs
Publication statusPublished - 15 Jan 2015

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Keywords

  • vascular endothelial growth factor (VEGF)
  • VEGF189
  • neuron
  • neuropilin
  • mouse

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