Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro

Eva Ramos Morales, N. McKain, R. M. A. Gawad, A. Hugo, R. J. Wallace

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P

Original languageEnglish
Pages (from-to)54-63
Number of pages10
JournalAnimal Feed Science and Technology
Volume222
Early online date3 Oct 2016
DOIs
Publication statusPublished - Dec 2016

Keywords

  • Biohydrogenation
  • Conjugated linoleic acid
  • Rumen
  • Vernolic acid
  • Vernonia galamensis
  • conjugated linoleic-acid
  • ruminal biohydrogenation
  • vaccenic acid
  • chrysanthemum-coronarium
  • marine-algae
  • stearic-acid
  • human health
  • dairy-cows
  • rumne
  • oil

Cite this

Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro. / Morales, Eva Ramos; McKain, N.; Gawad, R. M. A.; Hugo, A.; Wallace, R. J.

In: Animal Feed Science and Technology, Vol. 222, 12.2016, p. 54-63.

Research output: Contribution to journalArticle

Morales, Eva Ramos ; McKain, N. ; Gawad, R. M. A. ; Hugo, A. ; Wallace, R. J. / Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro. In: Animal Feed Science and Technology. 2016 ; Vol. 222. pp. 54-63.
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abstract = "Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P",
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AU - Hugo, A.

AU - Wallace, R. J.

N1 - Acknowledgements This work was financed partly by the EC Sixth Framework Programme project, REPLACE, Contract no: 506487. The Rowett Institute of Nutrition and Health receives funding from the Rural and Environment Science and Analytical Services division (RESAS) of the Scottish Government. The authors thank Susan Moir, Donna Henderson and David Brown for help with fatty acid analysis. We would like to thank Graham Horgan for his help with statistical analysis of data. We also thank the Regional Ministry for Innovation, Science and Enterprise, Andalusia, Spain for providing the scholarship to ERM.

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N2 - Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P

AB - Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P

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KW - Conjugated linoleic acid

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KW - Vernolic acid

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KW - conjugated linoleic-acid

KW - ruminal biohydrogenation

KW - vaccenic acid

KW - chrysanthemum-coronarium

KW - marine-algae

KW - stearic-acid

KW - human health

KW - dairy-cows

KW - rumne

KW - oil

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JO - Animal Feed Science and Technology

JF - Animal Feed Science and Technology

SN - 0377-8401

ER -