Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro

Eva Ramos Morales; N. McKain; R. M. A. Gawad; A. Hugo; R. J. Wallace

doi:10.1016/j.anifeedsci.2016.10.002

Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro

Eva Ramos Morales, N. McKain, R. M. A. Gawad, A. Hugo, R. J. Wallace

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P

Original language	English
Pages (from-to)	54-63
Number of pages	10
Journal	Animal Feed Science and Technology
Volume	222
Early online date	3 Oct 2016
DOIs	https://doi.org/10.1016/j.anifeedsci.2016.10.002
Publication status	Published - Dec 2016

Bibliographical note

Acknowledgements
This work was financed partly by the EC Sixth Framework Programme project, REPLACE, Contract no: 506487. The Rowett Institute of Nutrition and Health receives funding from the Rural and Environment Science and Analytical Services division (RESAS) of the Scottish Government.

The authors thank Susan Moir, Donna Henderson and David Brown for help with fatty acid analysis. We would like to thank Graham Horgan for his help with statistical analysis of data. We also thank the Regional Ministry for Innovation, Science and Enterprise, Andalusia, Spain for providing the scholarship to ERM.

Keywords

Biohydrogenation
Conjugated linoleic acid
Rumen
Vernolic acid
Vernonia galamensis
conjugated linoleic-acid
ruminal biohydrogenation
vaccenic acid
chrysanthemum-coronarium
marine-algae
stearic-acid
human health
dairy-cows
rumne
oil

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro",

abstract = "Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P",

keywords = "Biohydrogenation, Conjugated linoleic acid, Rumen, Vernolic acid, Vernonia galamensis, conjugated linoleic-acid, ruminal biohydrogenation, vaccenic acid, chrysanthemum-coronarium, marine-algae, stearic-acid, human health, dairy-cows, rumne, oil",

author = "Morales, {Eva Ramos} and N. McKain and Gawad, {R. M. A.} and A. Hugo and Wallace, {R. J.}",

note = "Acknowledgements This work was financed partly by the EC Sixth Framework Programme project, REPLACE, Contract no: 506487. The Rowett Institute of Nutrition and Health receives funding from the Rural and Environment Science and Analytical Services division (RESAS) of the Scottish Government. The authors thank Susan Moir, Donna Henderson and David Brown for help with fatty acid analysis. We would like to thank Graham Horgan for his help with statistical analysis of data. We also thank the Regional Ministry for Innovation, Science and Enterprise, Andalusia, Spain for providing the scholarship to ERM.",

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doi = "10.1016/j.anifeedsci.2016.10.002",

language = "English",

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journal = "Animal Feed Science and Technology",

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T1 - Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro

AU - Morales, Eva Ramos

AU - McKain, N.

AU - Gawad, R. M. A.

AU - Hugo, A.

AU - Wallace, R. J.

N1 - Acknowledgements This work was financed partly by the EC Sixth Framework Programme project, REPLACE, Contract no: 506487. The Rowett Institute of Nutrition and Health receives funding from the Rural and Environment Science and Analytical Services division (RESAS) of the Scottish Government. The authors thank Susan Moir, Donna Henderson and David Brown for help with fatty acid analysis. We would like to thank Graham Horgan for his help with statistical analysis of data. We also thank the Regional Ministry for Innovation, Science and Enterprise, Andalusia, Spain for providing the scholarship to ERM.

PY - 2016/12

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N2 - Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P

AB - Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P

KW - Biohydrogenation

KW - Conjugated linoleic acid

KW - Rumen

KW - Vernolic acid

KW - Vernonia galamensis

KW - conjugated linoleic-acid

KW - ruminal biohydrogenation

KW - vaccenic acid

KW - chrysanthemum-coronarium

KW - marine-algae

KW - stearic-acid

KW - human health

KW - dairy-cows

KW - rumne

KW - oil

U2 - 10.1016/j.anifeedsci.2016.10.002

DO - 10.1016/j.anifeedsci.2016.10.002

M3 - Article

SN - 0377-8401

VL - 222

SP - 54

EP - 63

JO - Animal Feed Science and Technology

JF - Animal Feed Science and Technology

ER -

Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro

Abstract

Bibliographical note

Keywords

UN SDGs

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