Abstract
Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P
| Original language | English |
|---|---|
| Pages (from-to) | 54-63 |
| Number of pages | 10 |
| Journal | Animal Feed Science and Technology |
| Volume | 222 |
| Early online date | 3 Oct 2016 |
| DOIs | |
| Publication status | Published - Dec 2016 |
Keywords
- Biohydrogenation
- Conjugated linoleic acid
- Rumen
- Vernolic acid
- Vernonia galamensis
- conjugated linoleic-acid
- ruminal biohydrogenation
- vaccenic acid
- chrysanthemum-coronarium
- marine-algae
- stearic-acid
- human health
- dairy-cows
- rumne
- oil
Cite this
Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro. / Morales, Eva Ramos; McKain, N.; Gawad, R. M. A.; Hugo, A.; Wallace, R. J.
In: Animal Feed Science and Technology, Vol. 222, 12.2016, p. 54-63.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro
AU - Morales, Eva Ramos
AU - McKain, N.
AU - Gawad, R. M. A.
AU - Hugo, A.
AU - Wallace, R. J.
N1 - Acknowledgements This work was financed partly by the EC Sixth Framework Programme project, REPLACE, Contract no: 506487. The Rowett Institute of Nutrition and Health receives funding from the Rural and Environment Science and Analytical Services division (RESAS) of the Scottish Government. The authors thank Susan Moir, Donna Henderson and David Brown for help with fatty acid analysis. We would like to thank Graham Horgan for his help with statistical analysis of data. We also thank the Regional Ministry for Innovation, Science and Enterprise, Andalusia, Spain for providing the scholarship to ERM.
PY - 2016/12
Y1 - 2016/12
N2 - Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P
AB - Substituted long-chain fatty acids may be useful dietary supplements to suppress ruminal biohydrogenation of unsaturated fatty acids (UFA) and thereby increase the flow of UFA to meat and milk. The aim of this study was to determine if Vernonia galamensis (ironweed, a member of the sunflower family) and vernolic acid (cis-12,13-epoxy-cis-9-octadecenoic acid), the main constituent of the seed oil, affected the biohydrogenation of linoleic acid (LA; cis-9,cis-12-18:2) to rumenic acid (CLA; cis-9, trans-11-18:2), vaccenic acid (VA;trans-11-18:1) and stearic acid (SA; 18:0) by ruminal microorganisms. Ruminal digesta from four sheep receiving a mixed hay-concentrate diet were incubated in vitro with LA (0.2 g/L) 0.2 g/L vernolic acid or 5 g/L of dried flowers or leaves of V. galamensis, either alone or combined. Vernolic acid had a substantial effect on LA metabolism, causing decreases in cis-9, trans-11 CLA and VA accumulation as well as SA production (P
KW - Biohydrogenation
KW - Conjugated linoleic acid
KW - Rumen
KW - Vernolic acid
KW - Vernonia galamensis
KW - conjugated linoleic-acid
KW - ruminal biohydrogenation
KW - vaccenic acid
KW - chrysanthemum-coronarium
KW - marine-algae
KW - stearic-acid
KW - human health
KW - dairy-cows
KW - rumne
KW - oil
U2 - 10.1016/j.anifeedsci.2016.10.002
DO - 10.1016/j.anifeedsci.2016.10.002
M3 - Article
VL - 222
SP - 54
EP - 63
JO - Animal Feed Science and Technology
JF - Animal Feed Science and Technology
SN - 0377-8401
ER -