What do microbial biosensors tell us about the behavior of MTBE?

H Maciel, G I Paton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Although highly soluble and ubiquitous in the terrestrial environment there is little known about the impact of MTBE on microbial ecotoxicity tests. Microbial ecotoxicity tests are important as they are rapid indicators of environmental health and are also able to diagnose constraints to contaminant mineralisation. MTBE is rarely found in isolation and may have effects in combination with other hydrocarbons. To investigate the impact of MTBE on microbial ecotoxicity tests, the response of a range of lux-based biosensors was measured against aqueous samples of MTBE, benzene, and naphthalene, both individually and in combination. Metabolic sensors (indicative of general environmental stress) and catabolic sensors (induced by specific analytes) were used and the response modeled, enabling pollutant interactions to be identified. All concentrations were confirmed by PTI-GC-FID or HPLC. MTBE did not increase the toxicity of benzene or naphthalene to two of the sensors used but may have altered the pollutant bioavailability and indeed for some analytes reduced toxicity significantly.

Original languageEnglish
Title of host publicationBIOREMEDIATION OF MTBE, ALCOHOLS, AND ETHERS
EditorsVS Magar, JT Gibbs, KT OReilly, MR Hyman, A Leeson
Place of PublicationCOLUMBUS
PublisherBattelle Press
Pages110
Number of pages10
ISBN (Print)1-57477-111-6
Publication statusPublished - 2001
Event6th International In Situ and On-Site Bioremediation Symposium - SAN DIEGO
Duration: 4 Jun 20017 Jun 2001

Conference

Conference6th International In Situ and On-Site Bioremediation Symposium
CitySAN DIEGO
Period4/06/017/06/01

Keywords

  • TOXICITY

Cite this

Maciel, H., & Paton, G. I. (2001). What do microbial biosensors tell us about the behavior of MTBE? In VS. Magar, JT. Gibbs, KT. OReilly, MR. Hyman, & A. Leeson (Eds.), BIOREMEDIATION OF MTBE, ALCOHOLS, AND ETHERS (pp. 110). Battelle Press.