Whole body protein metabolism in human pulmonary tuberculosis and undernutrition: evidence for anabolic block in tuberculosis

D C Macallan, M A McNurlan, A V Kurpad, G de Souza, P S Shetty, Alexander Graham Calder, G E Griffin

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    Abstract

    1. Differing patterns of protein metabolism are seen in wasting due to undernutrition and wasting due to chronic infection. 2. We investigated whole body energy and protein metabolism in nine subjects with pulmonary tuberculosis, six undernourished subjects (body mass index <18.5 kg/m2) and seven control subjects from an Indian population. Fasting subjects were infused with L-[1-13C]leucine (2.3 mumol.h-1.kg-1) for 8 h, 4 h fasted then 4 h fed. Leucine kinetics were derived from 13C-enrichment of leucine and alpha-ketoisocaproic acid in plasma and CO2 in breath. 3. Undernourished subjects, but not tuberculosis subjects, had higher rates of whole body protein turnover per unit lean body mass than controls [163.1 +/- 9.4 and 148.6 +/- 14.6 mumol compared with 142.8 +/- 14.7 mumol leucine/h per kg, based on alpha-ketoisocaproic acid enrichment (P = 0.039)]. 4. In response to feeding, protein oxidation increased in all groups. Tuberculosis subjects had the highest fed rates of oxidation (47.0 +/- 10.5 compared with 37.1 +/- 5.4 mumol.h-1.kg-1 in controls), resulting in a less positive net protein balance in the fed phase (controls, 39.7 +/- 6.2; undernourished subjects, 29.2 +/- 10.6; tuberculosis subjects, 24.5 +/- 9.3; P = 0.010). Thus fed-phase tuberculosis subjects oxidized a greater proportion of leucine flux (33.2%) than either of the other groups (controls, 24.0%; undernourished subjects, 24.0%; P = 0.017). 5. Tuberculosis did not increase fasting whole body protein turnover but impaired the anabolic response to feeding compared with control and undernourished subjects. Such 'anabolic block' may contribute to wasting in tuberculosis and may represent the mechanism by which some inflammatory states remain refractory to nutrition support.
    Original languageEnglish
    Pages (from-to)321-331
    Number of pages11
    JournalClinical Science
    Volume94
    Issue number3
    Publication statusPublished - 1 Mar 1998

    Keywords

    • Adolescent
    • Adult
    • Aged
    • Anthropometry
    • Cachexia
    • Cytokines
    • Deficiency Diseases
    • Energy Metabolism
    • Female
    • Humans
    • Keto Acids
    • Leucine
    • Male
    • Middle Aged
    • Oxidation-Reduction
    • Proteins
    • Tuberculosis, Pulmonary
    • tuberculosis
    • lung
    • malnutrition
    • metabolism
    • protein
    • whole body
    • energy
    • biosynthesis
    • biochemical analysis
    • comparative study
    • human
    • mycobacterial infection
    • bacteriosis
    • infection
    • respiratory disease
    • lung disease

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  • Cite this

    Macallan, D. C., McNurlan, M. A., Kurpad, A. V., de Souza, G., Shetty, P. S., Calder, A. G., & Griffin, G. E. (1998). Whole body protein metabolism in human pulmonary tuberculosis and undernutrition: evidence for anabolic block in tuberculosis. Clinical Science, 94(3), 321-331.