Whole Genome Amplification of Single Bacterial Cells

Paul Scott, Alan W. Walker

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recent developments in whole genome amplification techniques mean that it is now feasible to amplify enough DNA from just a single bacterial cell for downstream applications such as genome sequencing. As a result, single cell genomics is conceptually appealing as it can be used to characterise microbes without a prior requirement to cultivate them in the laboratory. While whole genome amplification methods are hugely powerful it is important to acknowledge that each approach also has inherent limitations, which impact on the quality of the resulting amplified DNA. In this chapter we discuss the various options available for whole genome amplification, and provide a standard protocol for whole genome amplification from individual bacterial cells. We also give a brief overview of the various steps involved in isolating single bacterial cells from mixed microbial communities, and in preparing them for genomic analyses.
Original languageEnglish
Title of host publicationHydrocarbon and Lipid Microbiology Protocols
Subtitle of host publicationIsolation and Cultivation
EditorsTerry J McGenity, Kenneth N. Timmis, Balbina Nogales Fernández
PublisherHumana Press
Pages29-41
Number of pages13
ISBN (Electronic)978-3-662-49131-7
ISBN (Print)978-3-662-49129-4
DOIs
Publication statusPublished - 21 Nov 2015

Publication series

NameSpringer Protocols Handbooks
ISSN (Print)1949-2448

Keywords

  • multiple displacement amplification
  • protocol
  • single cell genomics
  • whole genome amplification

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  • Cite this

    Scott, P., & Walker, A. W. (2015). Whole Genome Amplification of Single Bacterial Cells. In T. J. McGenity, K. N. Timmis, & B. Nogales Fernández (Eds.), Hydrocarbon and Lipid Microbiology Protocols: Isolation and Cultivation (pp. 29-41). (Springer Protocols Handbooks). Humana Press. https://doi.org/10.1007/8623_2015_152