Abstract
Whole genome sequences of four bacterial strains Dietzia maris SST1, Pseudomonas zhaodongensis SST2, Pseudomonas sp. SST3 and Halomonas sulfidaeris SST4, recovered from the South Shetland Trench sediment in Antarctica were analyzed using Ion Torrent sequencing technology. The respective sizes of their genomes (3.88, 4.99, 5.60 and 4.25 Mb) and GC contents (70.0, 60.3, 59.9 and 53.8%) are in agreement with these values of other strains of the species. The bacterial strains displayed promising antimicrobial activity against a number of pathogenic bacterial and fungal species. Whole genomes have been assembled and biosynthetic gene clusters (BGCs) have been identified using the antibiotics and Secondary Metabolite Analysis Shell (antiSMASH) web platform. Comparative analysis of the genome sequences revealed that the strains host abundant BGCs encoding for terpenes, siderophores, arylpolyene, bacteriocins, and lassopeptides. Furthermore, the key stress-related genes were identified and their distribution provided an insight into how these isolates adapt to key marine environmental conditions. This comprehensive study is a contribution to understanding the nature of life on the deep-sea environments.
Original language | English |
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Article number | 100782 |
Number of pages | 11 |
Journal | Marine Genomics |
Volume | 54 |
Early online date | 6 May 2020 |
DOIs | |
Publication status | Published - Dec 2020 |
Bibliographical note
L.H.A. thanks the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University for funding this work through the Fast-track Research Funding Program. The authors thank the Captain, crew and scientific party of the 2015-16 ‘PharmaDeep’ cruise on the Spanish Antarctic vessel BIO Hesperides. The cruise activity was supported by the European Union Research Infrastructures project ´EuroFleets 2´ under the 7th Framework Programme of the European Commission (agreement no. 312762). A.J.J. was the awardee of PharmaDeep: New Pharmaceuticals from the deep Antarctic. The authors also would like to thank John Still at ACEMAC for SEM images and the assay providers at Medina for their contribution.Keywords
- South Shetland Trench
- Dietzia sp.
- Pseudomonas sp.
- Halomonas sp.
- Genome sequencing
- biosynthetic gene clusters
- Antimicrobials
- Biosynthetic gene clusters