Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss

Raul Eduardo Pina Aguilar, Sandra G Martínez-Garza, Graciela Kohls, Marco A Vargas-Maciel, Luis G Vázquez de Lara, Claudia González-Ortega, Patricia Cancino-Villarreal, Antonio M Gutiérrez-Gutiérrez

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

AIM: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).

METHODS: Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.

RESULTS: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.

CONCLUSION: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.

Original languageEnglish
Pages (from-to)912-917
Number of pages6
JournalJournal of Obstetrics and Gynaecology
Volume38
Issue number6
Early online date9 Apr 2012
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Pregnancy
Sequence Tagged Sites
Andrology
Y Chromosome
Multiplex Polymerase Chain Reaction
Molecular Biology
Male sterility due to Y-chromosome deletions
Guidelines
Lymphocytes
DNA

Keywords

  • Abortion, Habitual
  • Adult
  • Chromosome Deletion
  • Chromosomes, Human, Y
  • Family Characteristics
  • Female
  • Genetic Testing
  • Humans
  • Male
  • Mexico
  • Middle Aged
  • Pregnancy
  • Sex Chromosome Aberrations
  • Sex Chromosome Disorders of Sex Development
  • Young Adult
  • Journal Article

Cite this

Pina Aguilar, R. E., Martínez-Garza, S. G., Kohls, G., Vargas-Maciel, M. A., Vázquez de Lara, L. G., González-Ortega, C., ... Gutiérrez-Gutiérrez, A. M. (2012). Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss. Journal of Obstetrics and Gynaecology, 38(6), 912-917. https://doi.org/10.1111/j.1447-0756.2011.01809.x

Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss. / Pina Aguilar, Raul Eduardo; Martínez-Garza, Sandra G; Kohls, Graciela; Vargas-Maciel, Marco A; Vázquez de Lara, Luis G; González-Ortega, Claudia; Cancino-Villarreal, Patricia; Gutiérrez-Gutiérrez, Antonio M.

In: Journal of Obstetrics and Gynaecology, Vol. 38, No. 6, 06.2012, p. 912-917.

Research output: Contribution to journalArticle

Pina Aguilar, RE, Martínez-Garza, SG, Kohls, G, Vargas-Maciel, MA, Vázquez de Lara, LG, González-Ortega, C, Cancino-Villarreal, P & Gutiérrez-Gutiérrez, AM 2012, 'Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss', Journal of Obstetrics and Gynaecology, vol. 38, no. 6, pp. 912-917. https://doi.org/10.1111/j.1447-0756.2011.01809.x
Pina Aguilar, Raul Eduardo ; Martínez-Garza, Sandra G ; Kohls, Graciela ; Vargas-Maciel, Marco A ; Vázquez de Lara, Luis G ; González-Ortega, Claudia ; Cancino-Villarreal, Patricia ; Gutiérrez-Gutiérrez, Antonio M. / Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss. In: Journal of Obstetrics and Gynaecology. 2012 ; Vol. 38, No. 6. pp. 912-917.
@article{9e844d35e1f84c06a181fbb65d96f2ad,
title = "Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss",
abstract = "AIM: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).METHODS: Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.RESULTS: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.CONCLUSION: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.",
keywords = "Abortion, Habitual, Adult, Chromosome Deletion, Chromosomes, Human, Y, Family Characteristics, Female, Genetic Testing, Humans, Male, Mexico, Middle Aged, Pregnancy, Sex Chromosome Aberrations, Sex Chromosome Disorders of Sex Development, Young Adult, Journal Article",
author = "{Pina Aguilar}, {Raul Eduardo} and Mart{\'i}nez-Garza, {Sandra G} and Graciela Kohls and Vargas-Maciel, {Marco A} and {V{\'a}zquez de Lara}, {Luis G} and Claudia Gonz{\'a}lez-Ortega and Patricia Cancino-Villarreal and Guti{\'e}rrez-Guti{\'e}rrez, {Antonio M}",
note = "{\circledC} 2012 The Authors. Journal of Obstetrics and Gynaecology Research {\circledC} 2012 Japan Society of Obstetrics and Gynecology.",
year = "2012",
month = "6",
doi = "10.1111/j.1447-0756.2011.01809.x",
language = "English",
volume = "38",
pages = "912--917",
journal = "Journal of Obstetrics and Gynaecology",
issn = "0144-3615",
publisher = "Informa Healthcare",
number = "6",

}

TY - JOUR

T1 - Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss

AU - Pina Aguilar, Raul Eduardo

AU - Martínez-Garza, Sandra G

AU - Kohls, Graciela

AU - Vargas-Maciel, Marco A

AU - Vázquez de Lara, Luis G

AU - González-Ortega, Claudia

AU - Cancino-Villarreal, Patricia

AU - Gutiérrez-Gutiérrez, Antonio M

N1 - © 2012 The Authors. Journal of Obstetrics and Gynaecology Research © 2012 Japan Society of Obstetrics and Gynecology.

PY - 2012/6

Y1 - 2012/6

N2 - AIM: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).METHODS: Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.RESULTS: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.CONCLUSION: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.

AB - AIM: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).METHODS: Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.RESULTS: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.CONCLUSION: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.

KW - Abortion, Habitual

KW - Adult

KW - Chromosome Deletion

KW - Chromosomes, Human, Y

KW - Family Characteristics

KW - Female

KW - Genetic Testing

KW - Humans

KW - Male

KW - Mexico

KW - Middle Aged

KW - Pregnancy

KW - Sex Chromosome Aberrations

KW - Sex Chromosome Disorders of Sex Development

KW - Young Adult

KW - Journal Article

U2 - 10.1111/j.1447-0756.2011.01809.x

DO - 10.1111/j.1447-0756.2011.01809.x

M3 - Article

VL - 38

SP - 912

EP - 917

JO - Journal of Obstetrics and Gynaecology

JF - Journal of Obstetrics and Gynaecology

SN - 0144-3615

IS - 6

ER -