Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss

Raul Eduardo Pina Aguilar; Sandra G Martínez-Garza; Graciela Kohls; Marco A Vargas-Maciel; Luis G Vázquez de Lara; Claudia González-Ortega; Patricia Cancino-Villarreal; Antonio M Gutiérrez-Gutiérrez

doi:10.1111/j.1447-0756.2011.01809.x

Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss

Raul Eduardo Pina Aguilar, Sandra G Martínez-Garza, Graciela Kohls, Marco A Vargas-Maciel, Luis G Vázquez de Lara, Claudia González-Ortega, Patricia Cancino-Villarreal, Antonio M Gutiérrez-Gutiérrez

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Abstract

AIM: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).

METHODS: Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.

RESULTS: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.

CONCLUSION: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.

Original language	English
Pages (from-to)	912-917
Number of pages	6
Journal	Journal of Obstetrics and Gynaecology
Volume	38
Issue number	6
Early online date	9 Apr 2012
DOIs	https://doi.org/10.1111/j.1447-0756.2011.01809.x
Publication status	Published - Jun 2012

Keywords

Abortion, Habitual
Adult
Chromosome Deletion
Chromosomes, Human, Y
Family Characteristics
Female
Genetic Testing
Humans
Male
Mexico
Middle Aged
Pregnancy
Sex Chromosome Aberrations
Sex Chromosome Disorders of Sex Development
Young Adult
Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Pina Aguilar, R. E., Martínez-Garza, S. G., Kohls, G., Vargas-Maciel, M. A., Vázquez de Lara, L. G., González-Ortega, C., Cancino-Villarreal, P., & Gutiérrez-Gutiérrez, A. M. (2012). Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss. Journal of Obstetrics and Gynaecology, 38(6), 912-917. https://doi.org/10.1111/j.1447-0756.2011.01809.x

Pina Aguilar, RE, Martínez-Garza, SG, Kohls, G, Vargas-Maciel, MA, Vázquez de Lara, LG, González-Ortega, C, Cancino-Villarreal, P & Gutiérrez-Gutiérrez, AM 2012, 'Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss', Journal of Obstetrics and Gynaecology, vol. 38, no. 6, pp. 912-917. https://doi.org/10.1111/j.1447-0756.2011.01809.x

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title = "Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss",

abstract = "AIM: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).METHODS: Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.RESULTS: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.CONCLUSION: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.",

keywords = "Abortion, Habitual, Adult, Chromosome Deletion, Chromosomes, Human, Y, Family Characteristics, Female, Genetic Testing, Humans, Male, Mexico, Middle Aged, Pregnancy, Sex Chromosome Aberrations, Sex Chromosome Disorders of Sex Development, Young Adult, Journal Article",

author = "{Pina Aguilar}, {Raul Eduardo} and Mart{\'i}nez-Garza, {Sandra G} and Graciela Kohls and Vargas-Maciel, {Marco A} and {V{\'a}zquez de Lara}, {Luis G} and Claudia Gonz{\'a}lez-Ortega and Patricia Cancino-Villarreal and Guti{\'e}rrez-Guti{\'e}rrez, {Antonio M}",

note = "{\textcopyright} 2012 The Authors. Journal of Obstetrics and Gynaecology Research {\textcopyright} 2012 Japan Society of Obstetrics and Gynecology.",

year = "2012",

month = jun,

doi = "10.1111/j.1447-0756.2011.01809.x",

language = "English",

volume = "38",

pages = "912--917",

journal = "Journal of Obstetrics and Gynaecology",

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TY - JOUR

T1 - Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss

AU - Pina Aguilar, Raul Eduardo

AU - Martínez-Garza, Sandra G

AU - Kohls, Graciela

AU - Vargas-Maciel, Marco A

AU - Vázquez de Lara, Luis G

AU - González-Ortega, Claudia

AU - Cancino-Villarreal, Patricia

AU - Gutiérrez-Gutiérrez, Antonio M

PY - 2012/6

Y1 - 2012/6

N2 - AIM: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).METHODS: Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.RESULTS: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.CONCLUSION: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.

AB - AIM: To analyze the presence of Y chromosome microdeletions in males of Mexican couples with idiopathic recurrent pregnancy losses (RPL).METHODS: Seventy-one males from couples with RPL and 66 fertile males as controls were studied. DNA was isolated from peripheral lymphocytes and used to run multiplex polymerase chain reactions. Regions AZFa (sY84, sY86), AZFb (sY127, sY134) and AZFc (sY254, sY255) of the Y chromosome were analyzed according to valid guidelines recommended by the European Academy of Andrology and the European Molecular Genetics Quality Network. Also, the sequence tagged sites (STSs): DYS262 (sY67), DYS220 (sY129), DYF85S1 (sY150), DYF86S1 (sY152) and DYF87S1 (sY153) were included in order to analyze STSs previously reported as deleted. A power analysis to support our simple size was performed.RESULTS: Results show an absence of Y chromosome microdeletions in males of couples with RPL and controls with an acceptable statistical power.CONCLUSION: The study did not show an association of recurrent pregnancy loss and Y chromosome microdeletions in Mexican male partners. Based on the results, the study of Y chromosome microdeletions in couples with RPL is not considered clinically relevant.

KW - Abortion, Habitual

KW - Adult

KW - Chromosome Deletion

KW - Chromosomes, Human, Y

KW - Family Characteristics

KW - Female

KW - Genetic Testing

KW - Humans

KW - Male

KW - Mexico

KW - Middle Aged

KW - Pregnancy

KW - Sex Chromosome Aberrations

KW - Sex Chromosome Disorders of Sex Development

KW - Young Adult

KW - Journal Article

U2 - 10.1111/j.1447-0756.2011.01809.x

DO - 10.1111/j.1447-0756.2011.01809.x

M3 - Article

C2 - 22486969

VL - 38

SP - 912

EP - 917

JO - Journal of Obstetrics and Gynaecology

JF - Journal of Obstetrics and Gynaecology

SN - 0144-3615

IS - 6

ER -

Y chromosome microdeletions in Mexican males of couples with idiopathic recurrent pregnancy loss

Abstract

Keywords

UN SDGs

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