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Andrew McEwan

Dr

    • AB25 2ZD

    • 180 Citations
    20062019
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    Personal profile

    Research Keywords:

    McEwan A. R.; Integrase; Phage; Streptomyces; Protein; DNA; Recombination; Integration; Macromolecular Complexes; Crystallography; EMSA; in vitro; in vivo;

    Research Interests:

    Post Doctoral Research in the Prof. M. C. M. Smith Group

    Institute of Medical Sciences, University of Aberdeen

    Investigation into the structure and function of phiC31 Streptomyces Phage Integrase (Int). Int is a member of the 'large serine recombinase' family. Int is required for integration of the phage genome into the host chromosome. This process of integration in highly site specific yet requires no additional co-factors or special conformations of DNA. The reverse reaction is equally specific in the presence of (the recently identified) Xis protein. The precise mechanism remains unknown. Int has been targeted as an application for use in gene therapies. Understanding of the mechanism and selectivity of Int is therefore essential if we are to adapt these proteins to specific sequences. Currently research has been directed towards a better understanding of the structure and function of these protein by studying the interactions between the protein and its substrates.  Recent work has been directed towards an analysis of mutations in both protein and substrate, through in vitro & in vivo recombination assays and electro-mobility shift assays and collaborations to determine other structural information such as high resolution structures.

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    Research Output 2006 2019

    • 180 Citations
    • 10 Article
    1 Downloads (Pure)
    Open Access
    File
    Addictive Behavior
    Cannabinoid Receptors
    Cannabinoids
    Clustered Regularly Interspaced Short Palindromic Repeats
    Anxiety
    4 Citations (Scopus)
    10 Downloads (Pure)

    A Blind Test of Computational Technique for Predicting the Likelihood of Peptide Sequences to Cyclize

    Booth, J., Alexandru-Crivac, C-N., Rickaby, K. A., Nneoyiegbe, A. F., Umeobika, U., McEwan, A. R., Trembleau, L., Jaspars, M., Houssen, W. E. & Shalashilin, D. V., 18 May 2017, In : The Journal of Physical Chemistry Letters. 8, 10, p. 2310-2315 6 p.

    Research output: Contribution to journalArticle

    Open Access
    File
    Cyclization
    Peptides
    peptides
    Conformations
    proximity
    12 Citations (Scopus)
    5 Downloads (Pure)

    Isolation and anti-HIV-1 integrase activity of lentzeosides A-F from extremotolerant lentzea sp. H45, a strain isolated from a high-altitude Atacama Desert soil

    Wichner, D., Idris, H., Houssen, W. E., McEwan, A. R., Bull, A. T., Asenjo, J. A., Goodfellow, M., Jaspars, M., Ebel, R. & Rateb, M. E., Apr 2017, In : The Journal of antibiotics. 70, p. 448-453 6 p.

    Research output: Contribution to journalArticle

    Open Access
    File
    HIV Integrase
    Actinobacteria
    Chile
    Glucosides
    Glycosides
    3 Citations (Scopus)
    5 Downloads (Pure)

    Accurate quantification of modified cyclic peptides without the need for authentic standards

    Adaba, R. I., Mann, G., Raab, A., Houssen, W. E., McEwan, A. R., Thomas, L., Tabudravu, J., Naismith, J. H. & Jaspars, M., 29 Dec 2016, In : Tetrahedron. 72, 52, p. 8603-8609 7 p.

    Research output: Contribution to journalArticle

    Open Access
    File
    34 Citations (Scopus)
    4 Downloads (Pure)

    An Efficient Method for the In Vitro Production of Azol(in)e-Based Cyclic Peptides

    Houssen, W. E., Bent, A. F., McEwan, A. R., Pieiller, N., Tabudravu, J., Koehnke, J., Mann, G., Adaba, R. I., Thomas, L., Hawas, U. W., Liu, H., Schwarz-Linek, U., Smith, M. C. M., Naismith, J. H. & Jaspars, M., 15 Dec 2014, In : Angewandte Chemie International Edition. 53, 51, p. 14171-14174 4 p.

    Research output: Contribution to journalArticle

    Open Access
    File