α-tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors

S C Robins, I Stewart, D E McNay, V Taylor, C Giachino, M Goetz, J Ninkovic, N Briancon, E Maratos-Flier, J S Flier, M V Kokoeva, M Placzek

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Abstract

Emerging evidence suggests that new cells, including neurons, can be generated within the adult hypothalamus, suggesting the existence of a local neural stem/progenitor cell niche. Here, we identify α-tanycytes as key components of a hypothalamic niche in the adult mouse. Long-term lineage tracing in vivo using a GLAST::CreER(T2) conditional driver indicates that α-tanycytes are self-renewing cells that constitutively give rise to new tanycytes, astrocytes and sparse numbers of neurons. In vitro studies demonstrate that α-tanycytes, but not β-tanycytes or parenchymal cells, are neurospherogenic. Distinct subpopulations of α-tanycytes exist, amongst which only GFAP-positive dorsal α2-tanycytes possess stem-like neurospherogenic activity. Fgf-10 and Fgf-18 are expressed specifically within ventral tanycyte subpopulations; α-tanycytes require fibroblast growth factor signalling to maintain their proliferation ex vivo and elevated fibroblast growth factor levels lead to enhanced proliferation of α-tanycytes in vivo. Our results suggest that α-tanycytes form the critical component of a hypothalamic stem cell niche, and that local fibroblast growth factor signalling governs their proliferation.

Original languageEnglish
Article number3049
Number of pages13
JournalNature Communications
Volume4
DOIs
Publication statusPublished - 27 Jun 2013

Fingerprint

Ependymoglial Cells
Third Ventricle
Fibroblast Growth Factors
fibroblasts
stem cells
Stem cells
neurons
Neurons
hypothalamus
cells
Population
Fermi Gamma-ray Space Telescope
tracing
stems
mice
emerging
Stem Cell Niche
Neural Stem Cells
Astrocytes
Hypothalamus

Keywords

  • aging
  • animals
  • cell proliferation
  • ependymoglial cells
  • epidermal growth factor
  • excitatory amino acid transporter 1
  • fibroblast growth factor 10
  • fibroblast growth factors
  • glial fibrillary acidic protein
  • hypothalamus
  • immunohistochemistry
  • integrases
  • mice
  • mice, inbred C57BL
  • neural stem cells
  • neuroglia
  • signal transduction
  • spheroids, cellular
  • third ventricle

Cite this

Robins, S. C., Stewart, I., McNay, D. E., Taylor, V., Giachino, C., Goetz, M., ... Placzek, M. (2013). α-tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors. Nature Communications, 4, [3049]. https://doi.org/10.1038/ncomms3049

α-tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors. / Robins, S C; Stewart, I; McNay, D E; Taylor, V; Giachino, C; Goetz, M; Ninkovic, J; Briancon, N; Maratos-Flier, E; Flier, J S; Kokoeva, M V; Placzek, M.

In: Nature Communications, Vol. 4, 3049, 27.06.2013.

Research output: Contribution to journalArticle

Robins, SC, Stewart, I, McNay, DE, Taylor, V, Giachino, C, Goetz, M, Ninkovic, J, Briancon, N, Maratos-Flier, E, Flier, JS, Kokoeva, MV & Placzek, M 2013, 'α-tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors', Nature Communications, vol. 4, 3049. https://doi.org/10.1038/ncomms3049
Robins, S C ; Stewart, I ; McNay, D E ; Taylor, V ; Giachino, C ; Goetz, M ; Ninkovic, J ; Briancon, N ; Maratos-Flier, E ; Flier, J S ; Kokoeva, M V ; Placzek, M. / α-tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors. In: Nature Communications. 2013 ; Vol. 4.
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