β(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen

J. Anjos, C. Fernandes, B.M.A. Silva, C. Quintas, A. Abrunheiro, N.A.R. Gow, T. Gonçalves

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Abstract

The fungal cell wall polymer β-(1,3)-D-glucan is synthesized by the enzyme β-(1,3)-D-glucan synthase that is a complex composed of at least two proteins, Rho1p and Fks1p. Here, we report the nucleotide sequence of a single FKS gene and of the regulatory unit, RHO1 from the dematiaceous pathogenic fungus Alternaria infectoria. The predicted AiFks and AiRho share, respectively, 93% and 100% identity with that of Drechslera tritici-repentis. We also report that the sensitivity to caspofungin of eight different A. infectoria clinical strains is similar, with a MIC > 32 μg/ml and a MEC of 1 μg/ml, except for one strain which had a MEC of 1.4 μg/ml. This same strain exhibited one substitution at the hot spot 2, S1405A, compatible with less susceptible phenotypes, with the other seven strains having no mutations in either hot spot 1 or 2. The relative quantification of the expression of AiFKS and of AiRHO demonstrated a decrease in response to an exposure to caspofungin at 0.5 μg/ml.
Original languageEnglish
Pages (from-to)716-725
Number of pages10
JournalMedical Mycology
Volume50
Issue number7
DOIs
Publication statusPublished - Oct 2012

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Anjos, J., Fernandes, C., Silva, B. M. A., Quintas, C., Abrunheiro, A., Gow, N. A. R., & Gonçalves, T. (2012). β(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen. Medical Mycology, 50(7), 716-725. https://doi.org/10.3109/13693786.2012.675525