β(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen

J. Anjos, C. Fernandes, B.M.A. Silva, C. Quintas, A. Abrunheiro, N.A.R. Gow, T. Gonçalves

Research output: Contribution to journalArticle

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Abstract

The fungal cell wall polymer β-(1,3)-D-glucan is synthesized by the enzyme β-(1,3)-D-glucan synthase that is a complex composed of at least two proteins, Rho1p and Fks1p. Here, we report the nucleotide sequence of a single FKS gene and of the regulatory unit, RHO1 from the dematiaceous pathogenic fungus Alternaria infectoria. The predicted AiFks and AiRho share, respectively, 93% and 100% identity with that of Drechslera tritici-repentis. We also report that the sensitivity to caspofungin of eight different A. infectoria clinical strains is similar, with a MIC > 32 μg/ml and a MEC of 1 μg/ml, except for one strain which had a MEC of 1.4 μg/ml. This same strain exhibited one substitution at the hot spot 2, S1405A, compatible with less susceptible phenotypes, with the other seven strains having no mutations in either hot spot 1 or 2. The relative quantification of the expression of AiFKS and of AiRHO demonstrated a decrease in response to an exposure to caspofungin at 0.5 μg/ml.
Original languageEnglish
Pages (from-to)716-725
Number of pages10
JournalMedical Mycology
Volume50
Issue number7
DOIs
Publication statusPublished - Oct 2012

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caspofungin
Alternaria
Regulator Genes
Cell Wall
Polymers
Fungi
Phenotype
Mutation
Enzymes
Proteins
glucan synthase
polyglucosan

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Anjos, J., Fernandes, C., Silva, B. M. A., Quintas, C., Abrunheiro, A., Gow, N. A. R., & Gonçalves, T. (2012). β(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen. Medical Mycology, 50(7), 716-725. https://doi.org/10.3109/13693786.2012.675525

β(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen. / Anjos, J.; Fernandes, C.; Silva, B.M.A.; Quintas, C.; Abrunheiro, A.; Gow, N.A.R.; Gonçalves, T.

In: Medical Mycology, Vol. 50, No. 7, 10.2012, p. 716-725.

Research output: Contribution to journalArticle

Anjos, J, Fernandes, C, Silva, BMA, Quintas, C, Abrunheiro, A, Gow, NAR & Gonçalves, T 2012, 'β(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen' Medical Mycology, vol. 50, no. 7, pp. 716-725. https://doi.org/10.3109/13693786.2012.675525
Anjos, J. ; Fernandes, C. ; Silva, B.M.A. ; Quintas, C. ; Abrunheiro, A. ; Gow, N.A.R. ; Gonçalves, T. / β(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen. In: Medical Mycology. 2012 ; Vol. 50, No. 7. pp. 716-725.
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