2-Deoxy-D-[1-3H]glucose uptake by MCF7 and T47D breast tumour cells under conditions of active proliferation and serum deprivation

Timothy Andrew Davies Smith, Jenny Titley, S A eccles, Ralph McReady

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Experimental biological therapies for cancer are being developed which interfere with growth factor activated cell signalling. Removal of serum from cultured MCF7 and T47D breast tumour cells is accompanied by decreased cell proliferation as a consequence of growth factor deprivation.
METHODS:
S-phase fraction and the uptake of 2-Deoxy-D-[1-3H]glucose by logarithmic MCF7 and T47D breast tumour cells was measured when cells were grown in the presence of serum and 24 hours after serum-deprivation.
RESULTS:
Removal of serum from early log phase T47D cells was associated with a decrease in both the proliferative fraction (S-phase) and the uptake of 2-deoxy-D-[1-3H]glucose compared with cells maintained in the presence of serum. However, as cells progressed through log phase growth the effect of serum-deprivation on S-phase fraction was less pronounced and there was no significant change in the uptake of 2-Deoxy-D-[1-3H]glucose between serum deprived and serum maintained T47D cells in late log phase. Essentially the same pattern was observed with MCF7 cells.
CONCLUSIONS:
The present study suggests that inhibition of cell growth by growth factor removal may be monitored by changes in DG uptake.
Original languageEnglish
Pages (from-to)1865-1870
Number of pages6
JournalAnticancer Research
Volume18
Issue number3A
Publication statusPublished - 1998

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Breast Neoplasms
Glucose
Serum
S Phase
Intercellular Signaling Peptides and Proteins
Investigational Therapies
Biological Therapy
MCF-7 Cells
Growth
Cell Proliferation
Neoplasms

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2-Deoxy-D-[1-3H]glucose uptake by MCF7 and T47D breast tumour cells under conditions of active proliferation and serum deprivation. / Smith, Timothy Andrew Davies; Titley, Jenny; eccles, S A; McReady, Ralph.

In: Anticancer Research, Vol. 18, No. 3A, 1998, p. 1865-1870.

Research output: Contribution to journalArticle

Smith, Timothy Andrew Davies ; Titley, Jenny ; eccles, S A ; McReady, Ralph. / 2-Deoxy-D-[1-3H]glucose uptake by MCF7 and T47D breast tumour cells under conditions of active proliferation and serum deprivation. In: Anticancer Research. 1998 ; Vol. 18, No. 3A. pp. 1865-1870.
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abstract = "Experimental biological therapies for cancer are being developed which interfere with growth factor activated cell signalling. Removal of serum from cultured MCF7 and T47D breast tumour cells is accompanied by decreased cell proliferation as a consequence of growth factor deprivation.METHODS:S-phase fraction and the uptake of 2-Deoxy-D-[1-3H]glucose by logarithmic MCF7 and T47D breast tumour cells was measured when cells were grown in the presence of serum and 24 hours after serum-deprivation.RESULTS:Removal of serum from early log phase T47D cells was associated with a decrease in both the proliferative fraction (S-phase) and the uptake of 2-deoxy-D-[1-3H]glucose compared with cells maintained in the presence of serum. However, as cells progressed through log phase growth the effect of serum-deprivation on S-phase fraction was less pronounced and there was no significant change in the uptake of 2-Deoxy-D-[1-3H]glucose between serum deprived and serum maintained T47D cells in late log phase. Essentially the same pattern was observed with MCF7 cells.CONCLUSIONS:The present study suggests that inhibition of cell growth by growth factor removal may be monitored by changes in DG uptake.",
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T1 - 2-Deoxy-D-[1-3H]glucose uptake by MCF7 and T47D breast tumour cells under conditions of active proliferation and serum deprivation

AU - Smith, Timothy Andrew Davies

AU - Titley, Jenny

AU - eccles, S A

AU - McReady, Ralph

PY - 1998

Y1 - 1998

N2 - Experimental biological therapies for cancer are being developed which interfere with growth factor activated cell signalling. Removal of serum from cultured MCF7 and T47D breast tumour cells is accompanied by decreased cell proliferation as a consequence of growth factor deprivation.METHODS:S-phase fraction and the uptake of 2-Deoxy-D-[1-3H]glucose by logarithmic MCF7 and T47D breast tumour cells was measured when cells were grown in the presence of serum and 24 hours after serum-deprivation.RESULTS:Removal of serum from early log phase T47D cells was associated with a decrease in both the proliferative fraction (S-phase) and the uptake of 2-deoxy-D-[1-3H]glucose compared with cells maintained in the presence of serum. However, as cells progressed through log phase growth the effect of serum-deprivation on S-phase fraction was less pronounced and there was no significant change in the uptake of 2-Deoxy-D-[1-3H]glucose between serum deprived and serum maintained T47D cells in late log phase. Essentially the same pattern was observed with MCF7 cells.CONCLUSIONS:The present study suggests that inhibition of cell growth by growth factor removal may be monitored by changes in DG uptake.

AB - Experimental biological therapies for cancer are being developed which interfere with growth factor activated cell signalling. Removal of serum from cultured MCF7 and T47D breast tumour cells is accompanied by decreased cell proliferation as a consequence of growth factor deprivation.METHODS:S-phase fraction and the uptake of 2-Deoxy-D-[1-3H]glucose by logarithmic MCF7 and T47D breast tumour cells was measured when cells were grown in the presence of serum and 24 hours after serum-deprivation.RESULTS:Removal of serum from early log phase T47D cells was associated with a decrease in both the proliferative fraction (S-phase) and the uptake of 2-deoxy-D-[1-3H]glucose compared with cells maintained in the presence of serum. However, as cells progressed through log phase growth the effect of serum-deprivation on S-phase fraction was less pronounced and there was no significant change in the uptake of 2-Deoxy-D-[1-3H]glucose between serum deprived and serum maintained T47D cells in late log phase. Essentially the same pattern was observed with MCF7 cells.CONCLUSIONS:The present study suggests that inhibition of cell growth by growth factor removal may be monitored by changes in DG uptake.

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