A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation

Carmen Valente, Gabriele Turacchio, Stefania Mariggiò, Alessandro Pagliuso, Renato Gaibisso, Giuseppe Di Tullio, Michele Santoro, Fabio Formiggini, Stefania Spanò, Daniele Piccini, Roman S Polishchuk, Antonino Colanzi, Alberto Luini, Daniela Corda

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Large pleiomorphic carriers leave the Golgi complex for the plasma membrane by en bloc extrusion of specialized tubular domains, which then undergo fission. Several components of the underlying molecular machinery have been identified, including those involved in the budding/initiation of tubular carrier precursors (for example, the phosphoinositide kinase PI(4)KIIIβ, the GTPase ARF, and FAPP2), and in the fission of these precursors (for example, PKD, CtBP1-S/BARS). However, how these proteins interact to bring about carrier formation is poorly understood. Here, we describe a protein complex that mediates carrier formation and contains budding and fission molecules, as well as other molecules, such as the adaptor protein 14-3-3γ. Specifically, we show that 14-3-3γ dimers bridge CtBP1-S/BARS with PI(4)KIIIβ, and that the resulting complex is stabilized by phosphorylation by PKD and PAK. Disrupting the association of these proteins inhibits the fission of elongating carrier precursors, indicating that this complex couples the carrier budding and fission processes.
Original languageEnglish
Pages (from-to)343-354
Number of pages12
JournalNature Cell Biology
Volume14
Issue number4
Early online date26 Feb 2012
DOIs
Publication statusPublished - Apr 2012

Fingerprint

14-3-3 Proteins
Proteins
1-Phosphatidylinositol 4-Kinase
GTP Phosphohydrolases
Golgi Apparatus
Phosphorylation
Cell Membrane

Keywords

  • 14-3-3 proteins
  • alcohol oxidoreductases
  • animals
  • COS cells
  • carrier proteins
  • cercopithecus aethiops
  • DNA-binding proteins
  • dimerization
  • golgi apparatus
  • humans
  • phosphorylation
  • phosphotransferases (alcohol group acceptor)
  • protein kinase C
  • rats
  • p21-activated kinases

Cite this

Valente, C., Turacchio, G., Mariggiò, S., Pagliuso, A., Gaibisso, R., Di Tullio, G., ... Corda, D. (2012). A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation. Nature Cell Biology, 14(4), 343-354. https://doi.org/10.1038/ncb2445

A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation. / Valente, Carmen; Turacchio, Gabriele; Mariggiò, Stefania; Pagliuso, Alessandro; Gaibisso, Renato; Di Tullio, Giuseppe; Santoro, Michele; Formiggini, Fabio; Spanò, Stefania; Piccini, Daniele; Polishchuk, Roman S; Colanzi, Antonino; Luini, Alberto; Corda, Daniela.

In: Nature Cell Biology, Vol. 14, No. 4, 04.2012, p. 343-354.

Research output: Contribution to journalArticle

Valente, C, Turacchio, G, Mariggiò, S, Pagliuso, A, Gaibisso, R, Di Tullio, G, Santoro, M, Formiggini, F, Spanò, S, Piccini, D, Polishchuk, RS, Colanzi, A, Luini, A & Corda, D 2012, 'A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation' Nature Cell Biology, vol. 14, no. 4, pp. 343-354. https://doi.org/10.1038/ncb2445
Valente C, Turacchio G, Mariggiò S, Pagliuso A, Gaibisso R, Di Tullio G et al. A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation. Nature Cell Biology. 2012 Apr;14(4):343-354. https://doi.org/10.1038/ncb2445
Valente, Carmen ; Turacchio, Gabriele ; Mariggiò, Stefania ; Pagliuso, Alessandro ; Gaibisso, Renato ; Di Tullio, Giuseppe ; Santoro, Michele ; Formiggini, Fabio ; Spanò, Stefania ; Piccini, Daniele ; Polishchuk, Roman S ; Colanzi, Antonino ; Luini, Alberto ; Corda, Daniela. / A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation. In: Nature Cell Biology. 2012 ; Vol. 14, No. 4. pp. 343-354.
@article{80963ea245624f97852a6abebaa25602,
title = "A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation",
abstract = "Large pleiomorphic carriers leave the Golgi complex for the plasma membrane by en bloc extrusion of specialized tubular domains, which then undergo fission. Several components of the underlying molecular machinery have been identified, including those involved in the budding/initiation of tubular carrier precursors (for example, the phosphoinositide kinase PI(4)KIIIβ, the GTPase ARF, and FAPP2), and in the fission of these precursors (for example, PKD, CtBP1-S/BARS). However, how these proteins interact to bring about carrier formation is poorly understood. Here, we describe a protein complex that mediates carrier formation and contains budding and fission molecules, as well as other molecules, such as the adaptor protein 14-3-3γ. Specifically, we show that 14-3-3γ dimers bridge CtBP1-S/BARS with PI(4)KIIIβ, and that the resulting complex is stabilized by phosphorylation by PKD and PAK. Disrupting the association of these proteins inhibits the fission of elongating carrier precursors, indicating that this complex couples the carrier budding and fission processes.",
keywords = "14-3-3 proteins, alcohol oxidoreductases, animals, COS cells, carrier proteins, cercopithecus aethiops, DNA-binding proteins, dimerization, golgi apparatus, humans, phosphorylation, phosphotransferases (alcohol group acceptor), protein kinase C, rats, p21-activated kinases",
author = "Carmen Valente and Gabriele Turacchio and Stefania Mariggi{\`o} and Alessandro Pagliuso and Renato Gaibisso and {Di Tullio}, Giuseppe and Michele Santoro and Fabio Formiggini and Stefania Span{\`o} and Daniele Piccini and Polishchuk, {Roman S} and Antonino Colanzi and Alberto Luini and Daniela Corda",
year = "2012",
month = "4",
doi = "10.1038/ncb2445",
language = "English",
volume = "14",
pages = "343--354",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - A 14-3-3γ dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIIIβ to regulate post-Golgi carrier formation

AU - Valente, Carmen

AU - Turacchio, Gabriele

AU - Mariggiò, Stefania

AU - Pagliuso, Alessandro

AU - Gaibisso, Renato

AU - Di Tullio, Giuseppe

AU - Santoro, Michele

AU - Formiggini, Fabio

AU - Spanò, Stefania

AU - Piccini, Daniele

AU - Polishchuk, Roman S

AU - Colanzi, Antonino

AU - Luini, Alberto

AU - Corda, Daniela

PY - 2012/4

Y1 - 2012/4

N2 - Large pleiomorphic carriers leave the Golgi complex for the plasma membrane by en bloc extrusion of specialized tubular domains, which then undergo fission. Several components of the underlying molecular machinery have been identified, including those involved in the budding/initiation of tubular carrier precursors (for example, the phosphoinositide kinase PI(4)KIIIβ, the GTPase ARF, and FAPP2), and in the fission of these precursors (for example, PKD, CtBP1-S/BARS). However, how these proteins interact to bring about carrier formation is poorly understood. Here, we describe a protein complex that mediates carrier formation and contains budding and fission molecules, as well as other molecules, such as the adaptor protein 14-3-3γ. Specifically, we show that 14-3-3γ dimers bridge CtBP1-S/BARS with PI(4)KIIIβ, and that the resulting complex is stabilized by phosphorylation by PKD and PAK. Disrupting the association of these proteins inhibits the fission of elongating carrier precursors, indicating that this complex couples the carrier budding and fission processes.

AB - Large pleiomorphic carriers leave the Golgi complex for the plasma membrane by en bloc extrusion of specialized tubular domains, which then undergo fission. Several components of the underlying molecular machinery have been identified, including those involved in the budding/initiation of tubular carrier precursors (for example, the phosphoinositide kinase PI(4)KIIIβ, the GTPase ARF, and FAPP2), and in the fission of these precursors (for example, PKD, CtBP1-S/BARS). However, how these proteins interact to bring about carrier formation is poorly understood. Here, we describe a protein complex that mediates carrier formation and contains budding and fission molecules, as well as other molecules, such as the adaptor protein 14-3-3γ. Specifically, we show that 14-3-3γ dimers bridge CtBP1-S/BARS with PI(4)KIIIβ, and that the resulting complex is stabilized by phosphorylation by PKD and PAK. Disrupting the association of these proteins inhibits the fission of elongating carrier precursors, indicating that this complex couples the carrier budding and fission processes.

KW - 14-3-3 proteins

KW - alcohol oxidoreductases

KW - animals

KW - COS cells

KW - carrier proteins

KW - cercopithecus aethiops

KW - DNA-binding proteins

KW - dimerization

KW - golgi apparatus

KW - humans

KW - phosphorylation

KW - phosphotransferases (alcohol group acceptor)

KW - protein kinase C

KW - rats

KW - p21-activated kinases

U2 - 10.1038/ncb2445

DO - 10.1038/ncb2445

M3 - Article

VL - 14

SP - 343

EP - 354

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 4

ER -