A Bright Future for Fluorescence Imaging of Fungi in Living Hosts

Ambre F. Chapuis, Elizabeth R. Ballou, Donna M. MacCallum (Corresponding Author)

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Abstract

Traditional in vivo investigation of fungal infection and new antifungal therapies in mouse models is usually carried out using post mortem methodologies. However, biomedical imaging techniques focusing on non-invasive techniques using bioluminescent and fluorescent proteins have become valuable tools. These new techniques address ethical concerns as they allow reduction in the number of animals required to evaluate new antifungal therapies. They also allow better understanding of the growth and spread of the pathogen during infection. In this review, we concentrate on imaging technologies using different fungal reporter proteins. We discuss the advantages and limitations of these different reporters and compare the efficacy of bioluminescent and fluorescent proteins for fungal research.
Original languageEnglish
Article number29
Number of pages9
JournalJournal of Fungi
Volume5
Issue number2
Early online date3 Apr 2019
DOIs
Publication statusPublished - 1 Jun 2019

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Keywords

  • flourescent reporters
  • bioluminescence
  • iRFP
  • live imaging
  • infection models
  • 3Rs
  • IRFP
  • Bioluminescence
  • Infection models
  • Live imaging
  • Fluorescent reporters

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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