Abstract
Whole cell inward currents activated by intracellular photorelease of cyclic guanosine monophosphate (cGMP) were investigated in cultured dorsal root ganglion (DRG) neurones. The actions of two distinct types of caged cGMP (NPE-caged cGMP and a highly water-soluble caged cGMP) were compared. Rapidly activating inward currents were evoked by cGMP in a subpopulation (12.5%) of neurones and these currents may be due to activity of cyclic nucleotide-gated channels. In contrast in 52% of DRG neurones intracellular photorelease of cGMP activated a delayed Ca2+-dependent inward current through the generation of cyclic ADPribose and mobilisation of Ca2+ from ryanodine sensitive intracellular stores. Similar delayed inward currents were activated by both caged compounds but only NPE-caged cGMP evoked rapidly activating currents. Cyclic GMP appears to increase excitability in some DRG neurones by diverse mechanisms. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Original language | English |
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Pages (from-to) | 143-146 |
Number of pages | 3 |
Journal | Neuroscience Letters |
Volume | 338 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2003 |
Keywords
- cyclic guanosine monophosphate
- ryanodine
- calcium mobilisation
- sensory neurone
- cyclic nucleotide-gated channel
- caged compounds
- cyclic ADPribose
- GLUTAMATE-RECEPTOR ACTIVATION
- NUCLEOTIDE-GATED CHANNELS
- ADP-RIBOSE
- CA2+