A comparison of cortical and trabecular bone from C57 Black 6 mice using Raman spectroscopy

Simon R. Goodyear; Iain R. Gibson; Jan Skakle; Richard P. K. Wells; Richard M. Aspden

doi:10.1016/j.bone.2009.01.008

A comparison of cortical and trabecular bone from C57 Black 6 mice using Raman spectroscopy

Simon R. Goodyear, Iain R. Gibson, Jan Skakle, Richard P. K. Wells, Richard M. Aspden

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Abstract

Cortical and trabecular bone are both produced and maintained by the same cell types. At the microscopic scale they have a similar lamellar structure but at a macroscopic scale they are very different. Raman microscopy has been used to investigate compositional differences in the two bone types using bone from standard laboratory mice in physiological conditions. Clear differences were observed when complete spectra were compared by principal component analysis (PCA). Analysis of individual bands showed cortical bone to have compositional characteristics of older bone when compared with trabecular material, possibly due to the higher bone turnover traditionally reported in the trabecular compartment. (C) 2009 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	899-907
Number of pages	9
Journal	Bone
Volume	44
Issue number	5
DOIs	https://doi.org/10.1016/j.bone.2009.01.008
Publication status	Published - May 2009

Keywords

Cortical bone
Trabecular bone
Mouse
Raman spectroscopy
Composition
protein secondary structure
elastic properties
Infrared-spectroscopy
mechanical-properties
Collagen
mineralization
tissue
microspectroscopy
nanoindentation
Raman spectroscopy

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10.1016/j.bone.2009.01.008

Manuscript final version
This is the author’s version of a work that was accepted for publication in Bone. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Bone, [VOL 44, ISSUE 5, (2009)] DOI10.1016/j.bone.2009.01.008
Accepted author manuscript, 342 KB

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title = "A comparison of cortical and trabecular bone from C57 Black 6 mice using Raman spectroscopy",

abstract = "Cortical and trabecular bone are both produced and maintained by the same cell types. At the microscopic scale they have a similar lamellar structure but at a macroscopic scale they are very different. Raman microscopy has been used to investigate compositional differences in the two bone types using bone from standard laboratory mice in physiological conditions. Clear differences were observed when complete spectra were compared by principal component analysis (PCA). Analysis of individual bands showed cortical bone to have compositional characteristics of older bone when compared with trabecular material, possibly due to the higher bone turnover traditionally reported in the trabecular compartment. (C) 2009 Elsevier Inc. All rights reserved.",

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AU - Aspden, Richard M.

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AB - Cortical and trabecular bone are both produced and maintained by the same cell types. At the microscopic scale they have a similar lamellar structure but at a macroscopic scale they are very different. Raman microscopy has been used to investigate compositional differences in the two bone types using bone from standard laboratory mice in physiological conditions. Clear differences were observed when complete spectra were compared by principal component analysis (PCA). Analysis of individual bands showed cortical bone to have compositional characteristics of older bone when compared with trabecular material, possibly due to the higher bone turnover traditionally reported in the trabecular compartment. (C) 2009 Elsevier Inc. All rights reserved.

KW - Cortical bone

KW - Trabecular bone

KW - Mouse

KW - Raman spectroscopy

KW - Composition

KW - protein secondary structure

KW - elastic properties

KW - Infrared-spectroscopy

KW - mechanical-properties

KW - Collagen

KW - mineralization

KW - tissue

KW - microspectroscopy

KW - nanoindentation

KW - Raman spectroscopy

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JO - Bone

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A comparison of cortical and trabecular bone from C57 Black 6 mice using Raman spectroscopy

Abstract

Keywords

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