A critical review of labelling techniques used to quantify rhizosphere carbon-flow

Andrew A. Meharg

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

The rhizosphere is a major sink for photo-assimilated carbon and quantifying inputs into this sink is one of the main goals of rhizosphere biology as organic carbon lost from plant roots supports a higher microbial population in the rhizosphere compared to bulk soil. Two fundamentally different (CO2)-C-14 labelling strategies have been developed to estimate carbon fluxes through the rhizosphere-continuous feeding of shoots with labelled carbon dioxide and pulse-chase experiments. The biological interpretation that can be placed on the results of labelling experiments is greatly biased by the technique used. It is the purpose of this paper to assess the advantages, disadvantages and the biological interpretation of both continuous and pulse labelling and to consider how to partition carbon fluxes within the rhizosphere.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalPlant and Soil
Volume166
Issue number1
DOIs
Publication statusPublished - 1994

Keywords

  • carbon-flow
  • carbon cycling
  • continuous labeling
  • pulse labeling
  • rhizosphere
  • roots
  • soil microbial biomass
  • root-derived material
  • festuca-pratensis L
  • lolium-perenne
  • assimilated carbon
  • substrate flow
  • fixed carbon
  • plant-roots
  • field plots
  • wheat

Cite this

A critical review of labelling techniques used to quantify rhizosphere carbon-flow. / Meharg, Andrew A.

In: Plant and Soil, Vol. 166, No. 1, 1994, p. 55-62.

Research output: Contribution to journalArticle

@article{23582345da4546b89de6508f495fe05f,
title = "A critical review of labelling techniques used to quantify rhizosphere carbon-flow",
abstract = "The rhizosphere is a major sink for photo-assimilated carbon and quantifying inputs into this sink is one of the main goals of rhizosphere biology as organic carbon lost from plant roots supports a higher microbial population in the rhizosphere compared to bulk soil. Two fundamentally different (CO2)-C-14 labelling strategies have been developed to estimate carbon fluxes through the rhizosphere-continuous feeding of shoots with labelled carbon dioxide and pulse-chase experiments. The biological interpretation that can be placed on the results of labelling experiments is greatly biased by the technique used. It is the purpose of this paper to assess the advantages, disadvantages and the biological interpretation of both continuous and pulse labelling and to consider how to partition carbon fluxes within the rhizosphere.",
keywords = "carbon-flow, carbon cycling, continuous labeling, pulse labeling, rhizosphere, roots, soil microbial biomass, root-derived material, festuca-pratensis L, lolium-perenne, assimilated carbon, substrate flow, fixed carbon, plant-roots, field plots, wheat",
author = "Meharg, {Andrew A.}",
year = "1994",
doi = "10.1007/BF02185481",
language = "English",
volume = "166",
pages = "55--62",
journal = "Plant and Soil",
issn = "0032-079X",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - A critical review of labelling techniques used to quantify rhizosphere carbon-flow

AU - Meharg, Andrew A.

PY - 1994

Y1 - 1994

N2 - The rhizosphere is a major sink for photo-assimilated carbon and quantifying inputs into this sink is one of the main goals of rhizosphere biology as organic carbon lost from plant roots supports a higher microbial population in the rhizosphere compared to bulk soil. Two fundamentally different (CO2)-C-14 labelling strategies have been developed to estimate carbon fluxes through the rhizosphere-continuous feeding of shoots with labelled carbon dioxide and pulse-chase experiments. The biological interpretation that can be placed on the results of labelling experiments is greatly biased by the technique used. It is the purpose of this paper to assess the advantages, disadvantages and the biological interpretation of both continuous and pulse labelling and to consider how to partition carbon fluxes within the rhizosphere.

AB - The rhizosphere is a major sink for photo-assimilated carbon and quantifying inputs into this sink is one of the main goals of rhizosphere biology as organic carbon lost from plant roots supports a higher microbial population in the rhizosphere compared to bulk soil. Two fundamentally different (CO2)-C-14 labelling strategies have been developed to estimate carbon fluxes through the rhizosphere-continuous feeding of shoots with labelled carbon dioxide and pulse-chase experiments. The biological interpretation that can be placed on the results of labelling experiments is greatly biased by the technique used. It is the purpose of this paper to assess the advantages, disadvantages and the biological interpretation of both continuous and pulse labelling and to consider how to partition carbon fluxes within the rhizosphere.

KW - carbon-flow

KW - carbon cycling

KW - continuous labeling

KW - pulse labeling

KW - rhizosphere

KW - roots

KW - soil microbial biomass

KW - root-derived material

KW - festuca-pratensis L

KW - lolium-perenne

KW - assimilated carbon

KW - substrate flow

KW - fixed carbon

KW - plant-roots

KW - field plots

KW - wheat

U2 - 10.1007/BF02185481

DO - 10.1007/BF02185481

M3 - Article

VL - 166

SP - 55

EP - 62

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 1

ER -