A guide to analysis of mouse energy metabolism

Matthias H Tschöp; John R Speakman; Jonathan R S Arch; Johan Auwerx; Jens C Brüning; Lawrence Chan; Robert H Eckel; Robert V Farese; Jose E Galgani; Catherine Hambly; Mark A Herman; Tamas L Horvath; Barbara B Kahn; Sara C Kozma; Eleftheria Maratos-Flier; Timo D Müller; Heike Münzberg; Paul T Pfluger; Leona Plum; Marc L Reitman; Kamal Rahmouni; Gerald I Shulman; George Thomas; C Ronald Kahn; Eric Ravussin

doi:10.1038/nmeth.1806

A guide to analysis of mouse energy metabolism

Matthias H Tschöp, John R Speakman, Jonathan R S Arch, Johan Auwerx, Jens C Brüning, Lawrence Chan, Robert H Eckel, Robert V Farese, Jose E Galgani, Catherine Hambly, Mark A Herman, Tamas L Horvath, Barbara B Kahn, Sara C Kozma, Eleftheria Maratos-Flier, Timo D Müller, Heike Münzberg, Paul T Pfluger, Leona Plum, Marc L ReitmanKamal Rahmouni, Gerald I Shulman, George Thomas, C Ronald Kahn, Eric Ravussin

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Abstract

We present a consolidated view of the complexity and challenges of designing studies for measurement of energy metabolism in mouse models, including a practical guide to the assessment of energy expenditure, energy intake and body composition and statistical analysis thereof. We hope this guide will facilitate comparisons across studies and minimize spurious interpretations of data. We recommend that division of energy expenditure data by either body weight or lean body weight and that presentation of group effects as histograms should be replaced by plotting individual data and analyzing both group and body-composition effects using analysis of covariance (ANCOVA).

Original language	English
Pages (from-to)	57-63
Number of pages	7
Journal	Nature methods
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/nmeth.1806
Publication status	Published - Jan 2012

Keywords

physiology
genetics
systems biology

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10.1038/nmeth.1806

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Tschöp, M. H., Speakman, J. R., Arch, J. R. S., Auwerx, J., Brüning, J. C., Chan, L., Eckel, R. H., Farese, R. V., Galgani, J. E., Hambly, C., Herman, M. A., Horvath, T. L., Kahn, B. B., Kozma, S. C., Maratos-Flier, E., Müller, T. D., Münzberg, H., Pfluger, P. T., Plum, L., ... Ravussin, E. (2012). A guide to analysis of mouse energy metabolism. Nature methods, 9(1), 57-63. https://doi.org/10.1038/nmeth.1806

Tschöp, MH, Speakman, JR, Arch, JRS, Auwerx, J, Brüning, JC, Chan, L, Eckel, RH, Farese, RV, Galgani, JE, Hambly, C, Herman, MA, Horvath, TL, Kahn, BB, Kozma, SC, Maratos-Flier, E, Müller, TD, Münzberg, H, Pfluger, PT, Plum, L, Reitman, ML, Rahmouni, K, Shulman, GI, Thomas, G, Kahn, CR & Ravussin, E 2012, 'A guide to analysis of mouse energy metabolism', Nature methods, vol. 9, no. 1, pp. 57-63. https://doi.org/10.1038/nmeth.1806

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AU - Eckel, Robert H

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KW - genetics

KW - systems biology

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A guide to analysis of mouse energy metabolism

Abstract

Keywords

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