A guide to analysis of mouse energy metabolism

Matthias H Tschöp, John R Speakman, Jonathan R S Arch, Johan Auwerx, Jens C Brüning, Lawrence Chan, Robert H Eckel, Robert V Farese, Jose E Galgani, Catherine Hambly, Mark A Herman, Tamas L Horvath, Barbara B Kahn, Sara C Kozma, Eleftheria Maratos-Flier, Timo D Müller, Heike Münzberg, Paul T Pfluger, Leona Plum, Marc L ReitmanKamal Rahmouni, Gerald I Shulman, George Thomas, C Ronald Kahn, Eric Ravussin

Research output: Contribution to journalArticle

376 Citations (Scopus)

Abstract

We present a consolidated view of the complexity and challenges of designing studies for measurement of energy metabolism in mouse models, including a practical guide to the assessment of energy expenditure, energy intake and body composition and statistical analysis thereof. We hope this guide will facilitate comparisons across studies and minimize spurious interpretations of data. We recommend that division of energy expenditure data by either body weight or lean body weight and that presentation of group effects as histograms should be replaced by plotting individual data and analyzing both group and body-composition effects using analysis of covariance (ANCOVA).
Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalNature methods
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 2012

Keywords

  • physiology
  • genetics
  • systems biology

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    Tschöp, M. H., Speakman, J. R., Arch, J. R. S., Auwerx, J., Brüning, J. C., Chan, L., Eckel, R. H., Farese, R. V., Galgani, J. E., Hambly, C., Herman, M. A., Horvath, T. L., Kahn, B. B., Kozma, S. C., Maratos-Flier, E., Müller, T. D., Münzberg, H., Pfluger, P. T., Plum, L., ... Ravussin, E. (2012). A guide to analysis of mouse energy metabolism. Nature methods, 9(1), 57-63. https://doi.org/10.1038/nmeth.1806