A miniaturised method for immunoglobulin allotype serology

Neil Thomas Young, J Street, C Darke

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A modification of the classic haemagglutination inhibition method for immunoglobulin allotyping is described which employs reagent volumes of 2 microliters compared with the 25 microliter volumes commonly used in other serological allotyping methods. No loss of sensitivity or specificity was associated with the miniaturised method when compared to the standard tile technique. This method simplifies the large scale phenotyping and screening of sera and permits considerable savings of valuable reagents to be made.
Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalJournal of Immunological Methods
Volume122
Issue number1
DOIs
Publication statusPublished - 15 Aug 1989

Fingerprint

Immunoglobulin Allotypes
Serology
Hemagglutination
Immunoglobulins
Sensitivity and Specificity
Serum

Keywords

  • Gene Frequency
  • Hemagglutination Inhibition Tests
  • Humans
  • Immunoglobulin Allotypes
  • Microchemistry
  • Wales
  • Miniaturized Method
  • Gm Allotype Frequency

Cite this

A miniaturised method for immunoglobulin allotype serology. / Young, Neil Thomas; Street, J; Darke, C.

In: Journal of Immunological Methods, Vol. 122, No. 1, 15.08.1989, p. 143-147.

Research output: Contribution to journalArticle

Young, Neil Thomas ; Street, J ; Darke, C. / A miniaturised method for immunoglobulin allotype serology. In: Journal of Immunological Methods. 1989 ; Vol. 122, No. 1. pp. 143-147.
@article{725b7f0d72344ab3bca20b33cf98e30b,
title = "A miniaturised method for immunoglobulin allotype serology",
abstract = "A modification of the classic haemagglutination inhibition method for immunoglobulin allotyping is described which employs reagent volumes of 2 microliters compared with the 25 microliter volumes commonly used in other serological allotyping methods. No loss of sensitivity or specificity was associated with the miniaturised method when compared to the standard tile technique. This method simplifies the large scale phenotyping and screening of sera and permits considerable savings of valuable reagents to be made.",
keywords = "Gene Frequency, Hemagglutination Inhibition Tests, Humans, Immunoglobulin Allotypes, Microchemistry, Wales, Miniaturized Method, Gm Allotype Frequency",
author = "Young, {Neil Thomas} and J Street and C Darke",
year = "1989",
month = "8",
day = "15",
doi = "10.1016/0022-1759(89)90345-1",
language = "English",
volume = "122",
pages = "143--147",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - A miniaturised method for immunoglobulin allotype serology

AU - Young, Neil Thomas

AU - Street, J

AU - Darke, C

PY - 1989/8/15

Y1 - 1989/8/15

N2 - A modification of the classic haemagglutination inhibition method for immunoglobulin allotyping is described which employs reagent volumes of 2 microliters compared with the 25 microliter volumes commonly used in other serological allotyping methods. No loss of sensitivity or specificity was associated with the miniaturised method when compared to the standard tile technique. This method simplifies the large scale phenotyping and screening of sera and permits considerable savings of valuable reagents to be made.

AB - A modification of the classic haemagglutination inhibition method for immunoglobulin allotyping is described which employs reagent volumes of 2 microliters compared with the 25 microliter volumes commonly used in other serological allotyping methods. No loss of sensitivity or specificity was associated with the miniaturised method when compared to the standard tile technique. This method simplifies the large scale phenotyping and screening of sera and permits considerable savings of valuable reagents to be made.

KW - Gene Frequency

KW - Hemagglutination Inhibition Tests

KW - Humans

KW - Immunoglobulin Allotypes

KW - Microchemistry

KW - Wales

KW - Miniaturized Method

KW - Gm Allotype Frequency

U2 - 10.1016/0022-1759(89)90345-1

DO - 10.1016/0022-1759(89)90345-1

M3 - Article

C2 - 2760475

VL - 122

SP - 143

EP - 147

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -