A PCR-based method for detecting the mycelia of stipitate hydnoid fungi in soil

Sietse van der Linde, Ian Alexander, Ian C. Anderson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To reduce the reliance on sporocarp records for conservation efforts, information on the below-ground distribution of specific fungal species, such as stipitate hydnoid fungi, is required. Species-specific primers were developed within the internal transcribed spacer (ITS1 and ITS2) regions for 12 hydnoid fungal species including Bankera fuligineoalba, Hydnellum aurantiacum, H. caeruleum, H. concrescens, H. ferrugineum, H. peckii, Phellodon confluens, P. melaleucus, P niger, P. tomentosus, Sarcodon glaucopus and S. squamosus. The specificity of the primer pairs was tested using BLAST searches and PCR amplifications. All primers amplified DNA only of the target species with the exception of those designed for P. melaleucus. In order to assess the ability of the primers to detect DNA from mycelium in soil, DNA extracted from soil samples taken from around solitary H. peckii sporocarps was amplified with the H. peckii primer 1peck and ITS2. H. peckii DNA was detected in 70% of all soil samples and up to 40 cm away from the base of individual sporocarps. The development of these species-specific primers provides a below-ground alternative for monitoring the distribution of these rare fungi. (C) 2008 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)40-46
Number of pages7
JournalJournal of Microbiological Methods
Volume75
Issue number1
Early online date15 May 2008
DOIs
Publication statusPublished - Sep 2008

Keywords

  • below-ground detection
  • ectomycorrhizal fungi
  • ECM mycelium
  • rare fungi
  • species-specific primers
  • stipitate hydnoid fungi
  • multiple sequence alignment
  • locked nucleic-acid
  • molecular-identification
  • DNA
  • quantification
  • discrimination
  • diversity
  • hydnellum
  • primers

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