A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects

Alexandre Pfister, Marie Barberon, Julien Alassimone, Lothar Kalmbach, Yuree Lee, Joop Em Vermeer, Misako Yamazaki, Guowei Li, Christophe Maurel, Junpei Takano, Takehiro Kamiya, David E Salt, Daniele Roppolo, Niko Geldner

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Abstract

The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)-major players of endodermal differentiation-into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.

Original languageEnglish
Article numbere03115
JournaleLife
Volume3
DOIs
Publication statusPublished - 16 Sep 2014

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Diffusion barriers
Nutrients
Homeostasis
Phosphotransferases
Food
Defects
Plant Roots
Potassium
Cell Membrane
Cell membranes
Proteins
Genes
Protein Domains

Cite this

Pfister, A., Barberon, M., Alassimone, J., Kalmbach, L., Lee, Y., Vermeer, J. E., ... Geldner, N. (2014). A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects. eLife, 3, [e03115]. https://doi.org/10.7554/eLife.03115

A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects. / Pfister, Alexandre; Barberon, Marie; Alassimone, Julien; Kalmbach, Lothar; Lee, Yuree; Vermeer, Joop Em; Yamazaki, Misako; Li, Guowei; Maurel, Christophe; Takano, Junpei; Kamiya, Takehiro; Salt, David E; Roppolo, Daniele; Geldner, Niko.

In: eLife, Vol. 3, e03115, 16.09.2014.

Research output: Contribution to journalArticle

Pfister, A, Barberon, M, Alassimone, J, Kalmbach, L, Lee, Y, Vermeer, JE, Yamazaki, M, Li, G, Maurel, C, Takano, J, Kamiya, T, Salt, DE, Roppolo, D & Geldner, N 2014, 'A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects', eLife, vol. 3, e03115. https://doi.org/10.7554/eLife.03115
Pfister, Alexandre ; Barberon, Marie ; Alassimone, Julien ; Kalmbach, Lothar ; Lee, Yuree ; Vermeer, Joop Em ; Yamazaki, Misako ; Li, Guowei ; Maurel, Christophe ; Takano, Junpei ; Kamiya, Takehiro ; Salt, David E ; Roppolo, Daniele ; Geldner, Niko. / A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects. In: eLife. 2014 ; Vol. 3.
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abstract = "The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)-major players of endodermal differentiation-into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.",
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T1 - A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects

AU - Pfister, Alexandre

AU - Barberon, Marie

AU - Alassimone, Julien

AU - Kalmbach, Lothar

AU - Lee, Yuree

AU - Vermeer, Joop Em

AU - Yamazaki, Misako

AU - Li, Guowei

AU - Maurel, Christophe

AU - Takano, Junpei

AU - Kamiya, Takehiro

AU - Salt, David E

AU - Roppolo, Daniele

AU - Geldner, Niko

N1 - We thank the Genomic Technologies Facility (GTF) and the Central Imaging Facility (CIF) of the University of Lausanne for expert technical support. We thank Valérie Dénervaud Tendon, Guillaume Germion, Deborah Mühlemann, and Kayo Konishi for technical assistance and John Danku and Véronique Vacchina for ICP-MS analysis. This work was funded by grants from the Swiss National Science Foundation (SNSF), the European Research Council (ERC) to NG and a Human Frontiers Science Program (HFSP) grant to JT and NG. GL and CM were supported by the Agropolis foundation (Rhizopolis) and the Agence Nationale de la Recherche (HydroRoot; ANR-11-BSV6-018). MB was supported by a EMBO long-term postdoctoral fellowship, JEMV by a Marie Curie IEF fellowship and TK by the Japan Society for the Promotion of Sciences (JSPS).

PY - 2014/9/16

Y1 - 2014/9/16

N2 - The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)-major players of endodermal differentiation-into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.

AB - The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)-major players of endodermal differentiation-into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.

U2 - 10.7554/eLife.03115

DO - 10.7554/eLife.03115

M3 - Article

VL - 3

JO - eLife

JF - eLife

SN - 2050-084X

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ER -