@article{1adfe5cfe117454aba4e9d1e249d0e7c,
title = "A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects",
abstract = "The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)-major players of endodermal differentiation-into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.",
author = "Alexandre Pfister and Marie Barberon and Julien Alassimone and Lothar Kalmbach and Yuree Lee and Vermeer, {Joop Em} and Misako Yamazaki and Guowei Li and Christophe Maurel and Junpei Takano and Takehiro Kamiya and Salt, {David E} and Daniele Roppolo and Niko Geldner",
note = "We thank the Genomic Technologies Facility (GTF) and the Central Imaging Facility (CIF) of the University of Lausanne for expert technical support. We thank Val{\'e}rie D{\'e}nervaud Tendon, Guillaume Germion, Deborah M{\"u}hlemann, and Kayo Konishi for technical assistance and John Danku and V{\'e}ronique Vacchina for ICP-MS analysis. This work was funded by grants from the Swiss National Science Foundation (SNSF), the European Research Council (ERC) to NG and a Human Frontiers Science Program (HFSP) grant to JT and NG. GL and CM were supported by the Agropolis foundation (Rhizopolis) and the Agence Nationale de la Recherche (HydroRoot; ANR-11-BSV6-018). MB was supported by a EMBO long-term postdoctoral fellowship, JEMV by a Marie Curie IEF fellowship and TK by the Japan Society for the Promotion of Sciences (JSPS). ",
year = "2014",
month = sep,
day = "16",
doi = "10.7554/eLife.03115",
language = "English",
volume = "3",
journal = "eLife",
publisher = "eLife Sciences Publications",
}