A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects

Alexandre Pfister, Marie Barberon, Julien Alassimone, Lothar Kalmbach, Yuree Lee, Joop Em Vermeer, Misako Yamazaki, Guowei Li, Christophe Maurel, Junpei Takano, Takehiro Kamiya, David E Salt, Daniele Roppolo, Niko Geldner

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Abstract

The endodermis represents the main barrier to extracellular diffusion in plant roots, and it is central to current models of plant nutrient uptake. Despite this, little is known about the genes setting up this endodermal barrier. In this study, we report the identification and characterization of a strong barrier mutant, schengen3 (sgn3). We observe a surprising ability of the mutant to maintain nutrient homeostasis, but demonstrate a major defect in maintaining sufficient levels of the macronutrient potassium. We show that SGN3/GASSHO1 is a receptor-like kinase that is necessary for localizing CASPARIAN STRIP DOMAIN PROTEINS (CASPs)-major players of endodermal differentiation-into an uninterrupted, ring-like domain. SGN3 appears to localize into a broader band, embedding growing CASP microdomains. The discovery of SGN3 strongly advances our ability to interrogate mechanisms of plant nutrient homeostasis and provides a novel actor for localized microdomain formation at the endodermal plasma membrane.

Original languageEnglish
Article numbere03115
JournaleLife
Volume3
DOIs
Publication statusPublished - 16 Sep 2014

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Pfister, A., Barberon, M., Alassimone, J., Kalmbach, L., Lee, Y., Vermeer, J. E., ... Geldner, N. (2014). A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects. eLife, 3, [e03115]. https://doi.org/10.7554/eLife.03115