A xylan hydrolase gene cluster in Prevotella ruminicola B(1)4: sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and beta-(1,4)-xylosidase activities

A Gasparic, Jennifer Cynthia Martin, A S Daniel, Harry James Flint

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65 Citations (Scopus)

Abstract

Two genes concerned with xylan degradation were found to be closely linked in the ruminal anaerobe Prevotella ruminicola B(1)4, being separated by an intergenic region of 75 nucleotides. xynA is shown to encode a family F endoxylanase of 369 amino acids, including a putative amino-terminal signal peptide. xynB encodes an enzyme of 319 amino acids, with no obvious signal peptide, that shows 68% amino acid identity with the xsa product of Bacteroides ovatus and 31% amino acid identity with a beta-xylosidase from Clostridium stercorarium; together, these three enzymes define a new family of beta-(1,4)-glycosidases. The activity of the cloned P. ruminicola xynB gene product, but not that of the xynA gene product, shows considerable sensitivity to oxygen. Studied under anaerobic conditions, the XynB enzyme was found to act as an exoxylanase, releasing xylose from substrates including xylobiose, xylopentaose, and birch wood xylan, but was relatively inactive against oat spelt xylan. A high degree of synergy (up to 10-fold stimulation) was found with respect to the release of reducing sugars from oat spelt xylan when XynB was combined with the XynA endoxylanase from P. ruminicola B(1)4 or with endoxylanases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17. Pretreatment with a fungal arabinofuranosidase also stimulated reducing-sugar release from xylans by XynB. In P. ruminicola the XynA and XynB enzymes may act sequentially in the breakdown of xylan.

Original languageEnglish
Pages (from-to)2958-64
Number of pages7
JournalApplied and Environmental Microbiology
Volume61
Issue number8
Publication statusPublished - Aug 1995

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Prevotella ruminicola
Xylosidases
Xylans
xylan
Multigene Family
hydrolases
multigene family
amino acid
endo-1,4-beta-xylanase
Endo-1,4-beta Xylanases
enzyme
Oxygen
oxygen
gene
Enzymes
enzymes
Triticum aestivum subsp. spelta
peptide
Amino Acids
sugar

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Bacterial
  • Endo-1,4-beta Xylanases
  • Genes, Bacterial
  • Genetic Linkage
  • Molecular Sequence Data
  • Multigene Family
  • Prevotella
  • Rumen
  • Sequence Homology, Amino Acid
  • Xylans
  • Xylosidases
  • beta-Glucosidase

Cite this

@article{9578102475454fad878e382e6aab95ce,
title = "A xylan hydrolase gene cluster in Prevotella ruminicola B(1)4:: sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and beta-(1,4)-xylosidase activities",
abstract = "Two genes concerned with xylan degradation were found to be closely linked in the ruminal anaerobe Prevotella ruminicola B(1)4, being separated by an intergenic region of 75 nucleotides. xynA is shown to encode a family F endoxylanase of 369 amino acids, including a putative amino-terminal signal peptide. xynB encodes an enzyme of 319 amino acids, with no obvious signal peptide, that shows 68{\%} amino acid identity with the xsa product of Bacteroides ovatus and 31{\%} amino acid identity with a beta-xylosidase from Clostridium stercorarium; together, these three enzymes define a new family of beta-(1,4)-glycosidases. The activity of the cloned P. ruminicola xynB gene product, but not that of the xynA gene product, shows considerable sensitivity to oxygen. Studied under anaerobic conditions, the XynB enzyme was found to act as an exoxylanase, releasing xylose from substrates including xylobiose, xylopentaose, and birch wood xylan, but was relatively inactive against oat spelt xylan. A high degree of synergy (up to 10-fold stimulation) was found with respect to the release of reducing sugars from oat spelt xylan when XynB was combined with the XynA endoxylanase from P. ruminicola B(1)4 or with endoxylanases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17. Pretreatment with a fungal arabinofuranosidase also stimulated reducing-sugar release from xylans by XynB. In P. ruminicola the XynA and XynB enzymes may act sequentially in the breakdown of xylan.",
keywords = "Amino Acid Sequence, Animals, Base Sequence, DNA, Bacterial, Endo-1,4-beta Xylanases, Genes, Bacterial, Genetic Linkage, Molecular Sequence Data, Multigene Family, Prevotella, Rumen, Sequence Homology, Amino Acid, Xylans, Xylosidases, beta-Glucosidase",
author = "A Gasparic and Martin, {Jennifer Cynthia} and Daniel, {A S} and Flint, {Harry James}",
year = "1995",
month = "8",
language = "English",
volume = "61",
pages = "2958--64",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "8",

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TY - JOUR

T1 - A xylan hydrolase gene cluster in Prevotella ruminicola B(1)4:

T2 - sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and beta-(1,4)-xylosidase activities

AU - Gasparic, A

AU - Martin, Jennifer Cynthia

AU - Daniel, A S

AU - Flint, Harry James

PY - 1995/8

Y1 - 1995/8

N2 - Two genes concerned with xylan degradation were found to be closely linked in the ruminal anaerobe Prevotella ruminicola B(1)4, being separated by an intergenic region of 75 nucleotides. xynA is shown to encode a family F endoxylanase of 369 amino acids, including a putative amino-terminal signal peptide. xynB encodes an enzyme of 319 amino acids, with no obvious signal peptide, that shows 68% amino acid identity with the xsa product of Bacteroides ovatus and 31% amino acid identity with a beta-xylosidase from Clostridium stercorarium; together, these three enzymes define a new family of beta-(1,4)-glycosidases. The activity of the cloned P. ruminicola xynB gene product, but not that of the xynA gene product, shows considerable sensitivity to oxygen. Studied under anaerobic conditions, the XynB enzyme was found to act as an exoxylanase, releasing xylose from substrates including xylobiose, xylopentaose, and birch wood xylan, but was relatively inactive against oat spelt xylan. A high degree of synergy (up to 10-fold stimulation) was found with respect to the release of reducing sugars from oat spelt xylan when XynB was combined with the XynA endoxylanase from P. ruminicola B(1)4 or with endoxylanases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17. Pretreatment with a fungal arabinofuranosidase also stimulated reducing-sugar release from xylans by XynB. In P. ruminicola the XynA and XynB enzymes may act sequentially in the breakdown of xylan.

AB - Two genes concerned with xylan degradation were found to be closely linked in the ruminal anaerobe Prevotella ruminicola B(1)4, being separated by an intergenic region of 75 nucleotides. xynA is shown to encode a family F endoxylanase of 369 amino acids, including a putative amino-terminal signal peptide. xynB encodes an enzyme of 319 amino acids, with no obvious signal peptide, that shows 68% amino acid identity with the xsa product of Bacteroides ovatus and 31% amino acid identity with a beta-xylosidase from Clostridium stercorarium; together, these three enzymes define a new family of beta-(1,4)-glycosidases. The activity of the cloned P. ruminicola xynB gene product, but not that of the xynA gene product, shows considerable sensitivity to oxygen. Studied under anaerobic conditions, the XynB enzyme was found to act as an exoxylanase, releasing xylose from substrates including xylobiose, xylopentaose, and birch wood xylan, but was relatively inactive against oat spelt xylan. A high degree of synergy (up to 10-fold stimulation) was found with respect to the release of reducing sugars from oat spelt xylan when XynB was combined with the XynA endoxylanase from P. ruminicola B(1)4 or with endoxylanases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17. Pretreatment with a fungal arabinofuranosidase also stimulated reducing-sugar release from xylans by XynB. In P. ruminicola the XynA and XynB enzymes may act sequentially in the breakdown of xylan.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - DNA, Bacterial

KW - Endo-1,4-beta Xylanases

KW - Genes, Bacterial

KW - Genetic Linkage

KW - Molecular Sequence Data

KW - Multigene Family

KW - Prevotella

KW - Rumen

KW - Sequence Homology, Amino Acid

KW - Xylans

KW - Xylosidases

KW - beta-Glucosidase

M3 - Article

C2 - 7487028

VL - 61

SP - 2958

EP - 2964

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 8

ER -