A2A adenosine receptors are located on presynaptic motor nerve terminals in the mouse

Rebecca L Baxter, Laura J Vega-Riveroll, Jim Deuchars, Simon H Parson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Extracellular adenosine is present at the mammalian neuromuscular junction (NMJ) by virtue of its release from activated nerve terminals and muscle fibers, and as a metabolite of adenosine tri-phosphate, which is coreleased with acetylcholine. Two activities for adenosine have been described: an inhibitory effect presumed to be modulated by the A1 receptor subtype, and a facilitatory effect mediated by the A2A receptor subtype. To date, only pharmacological evidence is available for these actions. We have used an antibody against the A2A receptor subtype, and demonstrated that A2A receptors are present on presynaptic motor nerve terminals at NMJs but not on associated glial or muscle cells, in the mouse. These results therefore provide additional evidence that there are multiple adenosine receptors present at the NMJ, and that stimulation of quantal and nonquantal release of acetylcholine (ACh) could be mediated by A2A receptors.

Original languageEnglish
Pages (from-to)229-34
Number of pages6
JournalSynapse
Volume57
Issue number4
DOIs
Publication statusPublished - 15 Sep 2005

Keywords

  • Animals
  • Female
  • Fluorescent Antibody Technique
  • Mice
  • Microscopy, Confocal
  • Motor Neurons
  • Muscle, Skeletal
  • Neuromuscular Junction
  • Presynaptic Terminals
  • Receptor, Adenosine A2A

Fingerprint Dive into the research topics of 'A2A adenosine receptors are located on presynaptic motor nerve terminals in the mouse'. Together they form a unique fingerprint.

  • Cite this