Acute Consumption of Flavan-3-ol-Enriched Dark Chocolate Affects Human Endogenous Metabolism

Luisa Martha Ostertag, Mark Philo, Ian J Colquhoun, Henri S Tapp, Shikha Saha, Garry G Duthie, E Kate Kemsley, Baukje de Roos, Paul A Kroon, Gwénaëlle Le Gall

Research output: Contribution to journalArticle

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Abstract

Flavan-3-ols and methylxanthines have potential beneficial effects on human health including reducing cardiovascular risk. We performed a randomized controlled cross-over intervention trial to assess the acute effects of consumption of flavan-3-ol-enriched dark chocolate, compared with standard dark chocolate and white chocolate, on the human metabolome. We assessed the metabolome in urine and blood plasma samples collected before and at 2 h and 6 h after consumption of chocolates in 42 healthy volunteers using a non-targeted metabolomics approach. Plasma samples were assessed and showed differentiation between time points with no further separation among the three chocolate treatments. Multivariate statistics applied to urine samples could readily separate the postprandial time points and distinguish between the treatments. Most of the markers responsible for the multivariate discrimination between the chocolates were of dietary origin. Interestingly, small but significant level changes were also observed for a subset of endogenous metabolites. 1H NMR revealed that flavan-3-ol-enriched dark chocolate and standard dark chocolate reduced urinary levels of creatinine, lactate, some amino acids and related degradation products and increased the levels of pyruvate and 4-hydroxyphenylacetate, a phenolic compound of bacterial origin. This study demonstrates that an acute chocolate intervention can significantly affect human metabolism.

Original languageEnglish
Pages (from-to)2516-2526
Number of pages11
JournalJournal of Proteome Research
Volume16
Issue number7
Early online date6 Jun 2017
DOIs
Publication statusPublished - 2017

Fingerprint

Metabolism
Plasmas
Metabolites
Pyruvic Acid
Lactic Acid
Creatinine
Blood
Nuclear magnetic resonance
Health
Statistics
Amino Acids
Degradation
Metabolome
Urine
flavan-3-ol
Chocolate
Metabolomics
Cross-Over Studies
Healthy Volunteers
4-hydroxyphenylacetate

Keywords

  • Journal Article
  • NMR
  • MS
  • metabolomics
  • chocolate
  • metabolism
  • health
  • flavonoids
  • methylxanthines

Cite this

Acute Consumption of Flavan-3-ol-Enriched Dark Chocolate Affects Human Endogenous Metabolism. / Ostertag, Luisa Martha; Philo, Mark; Colquhoun, Ian J; Tapp, Henri S; Saha, Shikha; Duthie, Garry G; Kemsley, E Kate; de Roos, Baukje; Kroon, Paul A; Le Gall, Gwénaëlle.

In: Journal of Proteome Research, Vol. 16, No. 7, 2017, p. 2516-2526.

Research output: Contribution to journalArticle

Ostertag, LM, Philo, M, Colquhoun, IJ, Tapp, HS, Saha, S, Duthie, GG, Kemsley, EK, de Roos, B, Kroon, PA & Le Gall, G 2017, 'Acute Consumption of Flavan-3-ol-Enriched Dark Chocolate Affects Human Endogenous Metabolism', Journal of Proteome Research, vol. 16, no. 7, pp. 2516-2526. https://doi.org/10.1021/acs.jproteome.7b00089
Ostertag, Luisa Martha ; Philo, Mark ; Colquhoun, Ian J ; Tapp, Henri S ; Saha, Shikha ; Duthie, Garry G ; Kemsley, E Kate ; de Roos, Baukje ; Kroon, Paul A ; Le Gall, Gwénaëlle. / Acute Consumption of Flavan-3-ol-Enriched Dark Chocolate Affects Human Endogenous Metabolism. In: Journal of Proteome Research. 2017 ; Vol. 16, No. 7. pp. 2516-2526.
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abstract = "Flavan-3-ols and methylxanthines have potential beneficial effects on human health including reducing cardiovascular risk. We performed a randomized controlled cross-over intervention trial to assess the acute effects of consumption of flavan-3-ol-enriched dark chocolate, compared with standard dark chocolate and white chocolate, on the human metabolome. We assessed the metabolome in urine and blood plasma samples collected before and at 2 h and 6 h after consumption of chocolates in 42 healthy volunteers using a non-targeted metabolomics approach. Plasma samples were assessed and showed differentiation between time points with no further separation among the three chocolate treatments. Multivariate statistics applied to urine samples could readily separate the postprandial time points and distinguish between the treatments. Most of the markers responsible for the multivariate discrimination between the chocolates were of dietary origin. Interestingly, small but significant level changes were also observed for a subset of endogenous metabolites. 1H NMR revealed that flavan-3-ol-enriched dark chocolate and standard dark chocolate reduced urinary levels of creatinine, lactate, some amino acids and related degradation products and increased the levels of pyruvate and 4-hydroxyphenylacetate, a phenolic compound of bacterial origin. This study demonstrates that an acute chocolate intervention can significantly affect human metabolism.",
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N2 - Flavan-3-ols and methylxanthines have potential beneficial effects on human health including reducing cardiovascular risk. We performed a randomized controlled cross-over intervention trial to assess the acute effects of consumption of flavan-3-ol-enriched dark chocolate, compared with standard dark chocolate and white chocolate, on the human metabolome. We assessed the metabolome in urine and blood plasma samples collected before and at 2 h and 6 h after consumption of chocolates in 42 healthy volunteers using a non-targeted metabolomics approach. Plasma samples were assessed and showed differentiation between time points with no further separation among the three chocolate treatments. Multivariate statistics applied to urine samples could readily separate the postprandial time points and distinguish between the treatments. Most of the markers responsible for the multivariate discrimination between the chocolates were of dietary origin. Interestingly, small but significant level changes were also observed for a subset of endogenous metabolites. 1H NMR revealed that flavan-3-ol-enriched dark chocolate and standard dark chocolate reduced urinary levels of creatinine, lactate, some amino acids and related degradation products and increased the levels of pyruvate and 4-hydroxyphenylacetate, a phenolic compound of bacterial origin. This study demonstrates that an acute chocolate intervention can significantly affect human metabolism.

AB - Flavan-3-ols and methylxanthines have potential beneficial effects on human health including reducing cardiovascular risk. We performed a randomized controlled cross-over intervention trial to assess the acute effects of consumption of flavan-3-ol-enriched dark chocolate, compared with standard dark chocolate and white chocolate, on the human metabolome. We assessed the metabolome in urine and blood plasma samples collected before and at 2 h and 6 h after consumption of chocolates in 42 healthy volunteers using a non-targeted metabolomics approach. Plasma samples were assessed and showed differentiation between time points with no further separation among the three chocolate treatments. Multivariate statistics applied to urine samples could readily separate the postprandial time points and distinguish between the treatments. Most of the markers responsible for the multivariate discrimination between the chocolates were of dietary origin. Interestingly, small but significant level changes were also observed for a subset of endogenous metabolites. 1H NMR revealed that flavan-3-ol-enriched dark chocolate and standard dark chocolate reduced urinary levels of creatinine, lactate, some amino acids and related degradation products and increased the levels of pyruvate and 4-hydroxyphenylacetate, a phenolic compound of bacterial origin. This study demonstrates that an acute chocolate intervention can significantly affect human metabolism.

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