Advanced glycation endproducts induce a proliferative response in vascular smooth muscle cells via altered calcium signaling

Kanola C. David, Roderick H. Scott, Graeme F. Nixon (Corresponding Author)

Research output: Contribution to journalArticle

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Abstract

Advanced glycation endproducts (AGEs) are proteins that accumulate in the plasma of diabetics as a result of increased glucose concentrations and are closely linked with vascular disease. The mechanisms involved are still not clear. The aim of this study was to investigate whether AGE-induced changes in calcium (Ca2+) homeostasis could contribute to these mechanisms. Cultured porcine coronary artery vascular smooth muscle (VSM) cells were preincubated with glycated albumin for 96 h. The sphingosine 1-phosphate (S1P)-induced intracellular Ca2+ increase, although not increased in amplitude, was significantly prolonged in cells preincubated with glycated albumin. Intracellular Ca2+ imaging and electrophysiological. recording of ion channel currents following release of caged Ca2+ indicated that this prolonged Ca2+ rise occurred predominantly via changes in Ca2+-induced Ca2+ release. Preincubation with glycated albumin also resulted in a threefold increase in expression of the receptor for AGE. As a consequence of the prolonged intracellular Ca2+ rise following preincubation with glycated albumin, the S1P-induced activation of the Ca2+- dependent phosphatase, calcineurin (CaN) was increased. This resulted in increased S1P-induced activation of the Ca2+-dependent transcription factor, nuclear factor of activated T cells (NFATc). BrdU incorporation in VSM cells was increased in cells preincubated with glycated albumin and was inhibited by the CaN inhibitor, cyclosporin A. In conclusion, AGE can induce VSM proliferation via a prolonged agonist-induced Ca2+ increase leading to increased activation of CaN and subsequently NFATc. This mechanism may contribute to pathogenesis of vascular disease in diabetes mellitus. (C) 2008 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)1110-1120
Number of pages11
JournalBiochemical Pharmacology
Volume76
Issue number9
Early online date19 Aug 2008
DOIs
Publication statusPublished - 30 Oct 2008

Keywords

  • vascular smooth muscle
  • diabetes
  • calcium
  • proliferation
  • glycation
  • activated T-cells
  • factor-kappa-B
  • end-products
  • nuclear-factor
  • endothelial-cells
  • diabetic complications
  • oxidative stress
  • ryanodine receptors
  • gene-expression
  • polyol pathway

Cite this

Advanced glycation endproducts induce a proliferative response in vascular smooth muscle cells via altered calcium signaling. / David, Kanola C.; Scott, Roderick H.; Nixon, Graeme F. (Corresponding Author).

In: Biochemical Pharmacology, Vol. 76, No. 9, 30.10.2008, p. 1110-1120.

Research output: Contribution to journalArticle

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AB - Advanced glycation endproducts (AGEs) are proteins that accumulate in the plasma of diabetics as a result of increased glucose concentrations and are closely linked with vascular disease. The mechanisms involved are still not clear. The aim of this study was to investigate whether AGE-induced changes in calcium (Ca2+) homeostasis could contribute to these mechanisms. Cultured porcine coronary artery vascular smooth muscle (VSM) cells were preincubated with glycated albumin for 96 h. The sphingosine 1-phosphate (S1P)-induced intracellular Ca2+ increase, although not increased in amplitude, was significantly prolonged in cells preincubated with glycated albumin. Intracellular Ca2+ imaging and electrophysiological. recording of ion channel currents following release of caged Ca2+ indicated that this prolonged Ca2+ rise occurred predominantly via changes in Ca2+-induced Ca2+ release. Preincubation with glycated albumin also resulted in a threefold increase in expression of the receptor for AGE. As a consequence of the prolonged intracellular Ca2+ rise following preincubation with glycated albumin, the S1P-induced activation of the Ca2+- dependent phosphatase, calcineurin (CaN) was increased. This resulted in increased S1P-induced activation of the Ca2+-dependent transcription factor, nuclear factor of activated T cells (NFATc). BrdU incorporation in VSM cells was increased in cells preincubated with glycated albumin and was inhibited by the CaN inhibitor, cyclosporin A. In conclusion, AGE can induce VSM proliferation via a prolonged agonist-induced Ca2+ increase leading to increased activation of CaN and subsequently NFATc. This mechanism may contribute to pathogenesis of vascular disease in diabetes mellitus. (C) 2008 Elsevier Inc. All rights reserved.

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KW - oxidative stress

KW - ryanodine receptors

KW - gene-expression

KW - polyol pathway

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