Advantages and limitations of a desolvation system coupled online to HPLC-ICPqMS/ES-MS for the quantitative determination of sulfur and arsenic in arseno-peptide complexes

Katharina Bluemlein, Eva Maria Krupp, Jorg Feldmann

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

High sulfur detection limits, mainly caused by the interfering O-2(+) ions, made simultaneous sulfur and arsenic speciation analysis by on-line coupling of RP-HPLC with ES-MS and ICP-MS rather difficult in the past. The application of a desolvation system avoids the formation of the interfering ions in the first place. In this study the Aridus system was tested. The sulfur detection limit of the used ICP-qMS in its standard mode could be improved by a factor 2000 to 50 ng S g(-1). But great care had to be taken regarding species independent quantification, the major advantage of ICP-MS, since the analyte transport of certain compounds such as methionine or arsenite to the ICP was affected by the desolvation system. However, sulfate was found to be a suitable calibrant for sulfur containing peptides such as phytochelatins. Hence, this method demonstrates for the first time, the potential to identify and quantify individual phytochelatins without phytochelatin standards. This is of importance, since no reliable and affordable phytochelatin standards are available, which would be necessary when molecular mass spectrometric methods would have been used solely. But a successful quantitative speciation analysis of free phytochelatins and their arsenite complexes was hampered due to peak broadening and more serious, the formation of arsenic-artefacts.

Original languageEnglish
Pages (from-to)108-113
Number of pages6
JournalJournal of Analytical Atomic Spectrometry
Volume24
Issue number1
DOIs
Publication statusPublished - 2009

Keywords

  • plasma-mass-spectrometry
  • performance liquid-chromatography
  • ellmans reagent
  • reaction cell
  • ICP-MS
  • phytochelatins
  • derivatization
  • ratios
  • plants

Cite this

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title = "Advantages and limitations of a desolvation system coupled online to HPLC-ICPqMS/ES-MS for the quantitative determination of sulfur and arsenic in arseno-peptide complexes",
abstract = "High sulfur detection limits, mainly caused by the interfering O-2(+) ions, made simultaneous sulfur and arsenic speciation analysis by on-line coupling of RP-HPLC with ES-MS and ICP-MS rather difficult in the past. The application of a desolvation system avoids the formation of the interfering ions in the first place. In this study the Aridus system was tested. The sulfur detection limit of the used ICP-qMS in its standard mode could be improved by a factor 2000 to 50 ng S g(-1). But great care had to be taken regarding species independent quantification, the major advantage of ICP-MS, since the analyte transport of certain compounds such as methionine or arsenite to the ICP was affected by the desolvation system. However, sulfate was found to be a suitable calibrant for sulfur containing peptides such as phytochelatins. Hence, this method demonstrates for the first time, the potential to identify and quantify individual phytochelatins without phytochelatin standards. This is of importance, since no reliable and affordable phytochelatin standards are available, which would be necessary when molecular mass spectrometric methods would have been used solely. But a successful quantitative speciation analysis of free phytochelatins and their arsenite complexes was hampered due to peak broadening and more serious, the formation of arsenic-artefacts.",
keywords = "plasma-mass-spectrometry, performance liquid-chromatography, ellmans reagent, reaction cell, ICP-MS, phytochelatins, derivatization, ratios, plants",
author = "Katharina Bluemlein and Krupp, {Eva Maria} and Jorg Feldmann",
year = "2009",
doi = "10.1039/b811600h",
language = "English",
volume = "24",
pages = "108--113",
journal = "Journal of Analytical Atomic Spectrometry",
issn = "0267-9477",
publisher = "The Royal Society of Chemistry",
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TY - JOUR

T1 - Advantages and limitations of a desolvation system coupled online to HPLC-ICPqMS/ES-MS for the quantitative determination of sulfur and arsenic in arseno-peptide complexes

AU - Bluemlein, Katharina

AU - Krupp, Eva Maria

AU - Feldmann, Jorg

PY - 2009

Y1 - 2009

N2 - High sulfur detection limits, mainly caused by the interfering O-2(+) ions, made simultaneous sulfur and arsenic speciation analysis by on-line coupling of RP-HPLC with ES-MS and ICP-MS rather difficult in the past. The application of a desolvation system avoids the formation of the interfering ions in the first place. In this study the Aridus system was tested. The sulfur detection limit of the used ICP-qMS in its standard mode could be improved by a factor 2000 to 50 ng S g(-1). But great care had to be taken regarding species independent quantification, the major advantage of ICP-MS, since the analyte transport of certain compounds such as methionine or arsenite to the ICP was affected by the desolvation system. However, sulfate was found to be a suitable calibrant for sulfur containing peptides such as phytochelatins. Hence, this method demonstrates for the first time, the potential to identify and quantify individual phytochelatins without phytochelatin standards. This is of importance, since no reliable and affordable phytochelatin standards are available, which would be necessary when molecular mass spectrometric methods would have been used solely. But a successful quantitative speciation analysis of free phytochelatins and their arsenite complexes was hampered due to peak broadening and more serious, the formation of arsenic-artefacts.

AB - High sulfur detection limits, mainly caused by the interfering O-2(+) ions, made simultaneous sulfur and arsenic speciation analysis by on-line coupling of RP-HPLC with ES-MS and ICP-MS rather difficult in the past. The application of a desolvation system avoids the formation of the interfering ions in the first place. In this study the Aridus system was tested. The sulfur detection limit of the used ICP-qMS in its standard mode could be improved by a factor 2000 to 50 ng S g(-1). But great care had to be taken regarding species independent quantification, the major advantage of ICP-MS, since the analyte transport of certain compounds such as methionine or arsenite to the ICP was affected by the desolvation system. However, sulfate was found to be a suitable calibrant for sulfur containing peptides such as phytochelatins. Hence, this method demonstrates for the first time, the potential to identify and quantify individual phytochelatins without phytochelatin standards. This is of importance, since no reliable and affordable phytochelatin standards are available, which would be necessary when molecular mass spectrometric methods would have been used solely. But a successful quantitative speciation analysis of free phytochelatins and their arsenite complexes was hampered due to peak broadening and more serious, the formation of arsenic-artefacts.

KW - plasma-mass-spectrometry

KW - performance liquid-chromatography

KW - ellmans reagent

KW - reaction cell

KW - ICP-MS

KW - phytochelatins

KW - derivatization

KW - ratios

KW - plants

U2 - 10.1039/b811600h

DO - 10.1039/b811600h

M3 - Article

VL - 24

SP - 108

EP - 113

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

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ER -