An alternative histochemical method to simultaneously demonstrate muscle nuclei and muscle fibre type

G. K. Sakkas, Derek Ball, T. H. Mercer, P. F. Naish

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We present a modified histochemical method to examine, simultaneously, nuclei and fibre type in human skeletal muscle. The new procedure (Haem-ATPase) is based on two previously used histochemical protocols. Biopsies were obtained from the rectus abdominis muscle of patients undergoing elective abdominal surgery. Fibre type composition, cross-sectional area (CSA) and nuclei to fibre ratio (N:F) were determined from frozen sections of each biopsy. To test the validity of the new method, serial sections of each biopsy were stained separately using the standard and modified methods. No differences were found in fibre type distribution, mean-weighted CSA and N:F when comparing the modified method with the standard methods. The Haem-ATPase method was found to shrink fibre size by at least 3% (P>0.05) compared with the established myosin acid labile method. We propose that this modified technique is suitable for initial examination of both the nuclei and fibre type in the same frozen sections of human skeletal muscle.

Original languageEnglish
Pages (from-to)503-505
Number of pages2
JournalEuropean Journal of Applied Physiology
Volume89
DOIs
Publication statusPublished - 2003

Keywords

  • myosin ATPase
  • haematoxylin
  • skeletal muscle
  • fibre type
  • nuclei

Cite this

An alternative histochemical method to simultaneously demonstrate muscle nuclei and muscle fibre type. / Sakkas, G. K.; Ball, Derek; Mercer, T. H.; Naish, P. F.

In: European Journal of Applied Physiology, Vol. 89, 2003, p. 503-505.

Research output: Contribution to journalArticle

@article{993201937e6d428cb24f44dc6c9ea1c1,
title = "An alternative histochemical method to simultaneously demonstrate muscle nuclei and muscle fibre type",
abstract = "We present a modified histochemical method to examine, simultaneously, nuclei and fibre type in human skeletal muscle. The new procedure (Haem-ATPase) is based on two previously used histochemical protocols. Biopsies were obtained from the rectus abdominis muscle of patients undergoing elective abdominal surgery. Fibre type composition, cross-sectional area (CSA) and nuclei to fibre ratio (N:F) were determined from frozen sections of each biopsy. To test the validity of the new method, serial sections of each biopsy were stained separately using the standard and modified methods. No differences were found in fibre type distribution, mean-weighted CSA and N:F when comparing the modified method with the standard methods. The Haem-ATPase method was found to shrink fibre size by at least 3{\%} (P>0.05) compared with the established myosin acid labile method. We propose that this modified technique is suitable for initial examination of both the nuclei and fibre type in the same frozen sections of human skeletal muscle.",
keywords = "myosin ATPase, haematoxylin, skeletal muscle, fibre type, nuclei",
author = "Sakkas, {G. K.} and Derek Ball and Mercer, {T. H.} and Naish, {P. F.}",
year = "2003",
doi = "10.1007/s00421-003-0829-z",
language = "English",
volume = "89",
pages = "503--505",
journal = "European Journal of Applied Physiology",
issn = "1439-6319",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - An alternative histochemical method to simultaneously demonstrate muscle nuclei and muscle fibre type

AU - Sakkas, G. K.

AU - Ball, Derek

AU - Mercer, T. H.

AU - Naish, P. F.

PY - 2003

Y1 - 2003

N2 - We present a modified histochemical method to examine, simultaneously, nuclei and fibre type in human skeletal muscle. The new procedure (Haem-ATPase) is based on two previously used histochemical protocols. Biopsies were obtained from the rectus abdominis muscle of patients undergoing elective abdominal surgery. Fibre type composition, cross-sectional area (CSA) and nuclei to fibre ratio (N:F) were determined from frozen sections of each biopsy. To test the validity of the new method, serial sections of each biopsy were stained separately using the standard and modified methods. No differences were found in fibre type distribution, mean-weighted CSA and N:F when comparing the modified method with the standard methods. The Haem-ATPase method was found to shrink fibre size by at least 3% (P>0.05) compared with the established myosin acid labile method. We propose that this modified technique is suitable for initial examination of both the nuclei and fibre type in the same frozen sections of human skeletal muscle.

AB - We present a modified histochemical method to examine, simultaneously, nuclei and fibre type in human skeletal muscle. The new procedure (Haem-ATPase) is based on two previously used histochemical protocols. Biopsies were obtained from the rectus abdominis muscle of patients undergoing elective abdominal surgery. Fibre type composition, cross-sectional area (CSA) and nuclei to fibre ratio (N:F) were determined from frozen sections of each biopsy. To test the validity of the new method, serial sections of each biopsy were stained separately using the standard and modified methods. No differences were found in fibre type distribution, mean-weighted CSA and N:F when comparing the modified method with the standard methods. The Haem-ATPase method was found to shrink fibre size by at least 3% (P>0.05) compared with the established myosin acid labile method. We propose that this modified technique is suitable for initial examination of both the nuclei and fibre type in the same frozen sections of human skeletal muscle.

KW - myosin ATPase

KW - haematoxylin

KW - skeletal muscle

KW - fibre type

KW - nuclei

U2 - 10.1007/s00421-003-0829-z

DO - 10.1007/s00421-003-0829-z

M3 - Article

VL - 89

SP - 503

EP - 505

JO - European Journal of Applied Physiology

JF - European Journal of Applied Physiology

SN - 1439-6319

ER -