An insight into piscidins

The discovery, modulation and bioactivity of greater amberjack, Seriola dumerili, piscidin

Douglas John Milne, Álvaro Fernández-Montero, Manu K Gundappa, Tiehui Wang, Félix Acosta, Silvia Torrecillas, Daniel Montero, Jun Zou, John Sweetman, Christopher John Secombes (Corresponding Author)

Research output: Contribution to journalArticle

Abstract

Antimicrobial peptides (AMPs) play an important role in the innate immune response of vertebrates by creating a hostile environment for any invading pathogens. Piscidins are potent teleost specific AMPs, which have a broad spectrum activity. We have identified a novel piscidin active peptide, in the greater amberjack, Seriola dumerili, that consists of 25 aa, which forms an amphipathic helix with distinct hydrophobic and positively charged regions. Following homology and phylogenetic analysis the greater amberjack piscidin was deemed to belong to the group 3 family of piscidins. Piscidin was expressed constitutively at immune sites, with transcript level highest in the spleen and gut, at an intermediate level in the gills and lowest in the head kidney. Following in vivo stimulation with PAMPs (poly I:C, LPS and flagellin) piscidin transcript level increased in gills in response to flagellin, in gut and spleen in response to poly I:C, and in head kidney in response to poly I:C, LPS and flagellin. Head kidney and spleen cells were then isolated from greater amberjack and incubated with each of the PAMPs for 4, 12 and 24 h. Piscidin expression was unchanged at 4 and 12 h post PAMP stimulation in head kidney cells but at 24 h transcript level was markedly upregulated compared to control (unstimulated) cells, especially with the bacterial PAMPs. In contrast, spleen cells upregulated piscidin expression by 4 h post stimulation with poly I:C and flagellin, and remained upregulated to 24 h with flagellin exposure, but had returned to baseline levels by 12 h using poly I:C. To determine if piscidin expression could be modulated by diet, greater amberjack were fed diets supplemented with MOS and cMOS for 30 days when transcript level was determined. It was found that MOS supplemented diets increased expression in the spleen, cMOS supplemented diets upregulated transcript levels in the gills and head kidney, whilst a diet containing both MOS and cMOS upregulated transcript in the gut, when compared to fish fed the control diet. Finally, a synthetic greater amberjack piscidin was produced and showed bacteriostatic activity against a number of bacterial strains, including both Gram positive and Gram negative fish pathogens.
Original languageEnglish
Pages (from-to)378-388
Number of pages11
JournalMolecular Immunology
Volume114
Early online date23 Aug 2019
DOIs
Publication statusPublished - Oct 2019

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Head Kidney
Flagellin
Poly I-C
Diet
Spleen
Peptides
Fishes
Innate Immunity
Vertebrates
Pathogen-Associated Molecular Pattern Molecules

Keywords

  • Greater amberjack
  • piscidin
  • innate immunity
  • PAMP
  • bacteriostatic activity

Cite this

An insight into piscidins : The discovery, modulation and bioactivity of greater amberjack, Seriola dumerili, piscidin. / Milne, Douglas John; Fernández-Montero, Álvaro; Gundappa, Manu K; Wang, Tiehui; Acosta, Félix; Torrecillas, Silvia; Montero, Daniel; Zou, Jun; Sweetman, John; Secombes, Christopher John (Corresponding Author).

In: Molecular Immunology, Vol. 114, 10.2019, p. 378-388.

Research output: Contribution to journalArticle

Milne, DJ, Fernández-Montero, Á, Gundappa, MK, Wang, T, Acosta, F, Torrecillas, S, Montero, D, Zou, J, Sweetman, J & Secombes, CJ 2019, 'An insight into piscidins: The discovery, modulation and bioactivity of greater amberjack, Seriola dumerili, piscidin', Molecular Immunology, vol. 114, pp. 378-388. https://doi.org/10.1016/j.molimm.2019.08.005
Milne, Douglas John ; Fernández-Montero, Álvaro ; Gundappa, Manu K ; Wang, Tiehui ; Acosta, Félix ; Torrecillas, Silvia ; Montero, Daniel ; Zou, Jun ; Sweetman, John ; Secombes, Christopher John. / An insight into piscidins : The discovery, modulation and bioactivity of greater amberjack, Seriola dumerili, piscidin. In: Molecular Immunology. 2019 ; Vol. 114. pp. 378-388.
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AU - Zou, Jun

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N2 - Antimicrobial peptides (AMPs) play an important role in the innate immune response of vertebrates by creating a hostile environment for any invading pathogens. Piscidins are potent teleost specific AMPs, which have a broad spectrum activity. We have identified a novel piscidin active peptide, in the greater amberjack, Seriola dumerili, that consists of 25 aa, which forms an amphipathic helix with distinct hydrophobic and positively charged regions. Following homology and phylogenetic analysis the greater amberjack piscidin was deemed to belong to the group 3 family of piscidins. Piscidin was expressed constitutively at immune sites, with transcript level highest in the spleen and gut, at an intermediate level in the gills and lowest in the head kidney. Following in vivo stimulation with PAMPs (poly I:C, LPS and flagellin) piscidin transcript level increased in gills in response to flagellin, in gut and spleen in response to poly I:C, and in head kidney in response to poly I:C, LPS and flagellin. Head kidney and spleen cells were then isolated from greater amberjack and incubated with each of the PAMPs for 4, 12 and 24 h. Piscidin expression was unchanged at 4 and 12 h post PAMP stimulation in head kidney cells but at 24 h transcript level was markedly upregulated compared to control (unstimulated) cells, especially with the bacterial PAMPs. In contrast, spleen cells upregulated piscidin expression by 4 h post stimulation with poly I:C and flagellin, and remained upregulated to 24 h with flagellin exposure, but had returned to baseline levels by 12 h using poly I:C. To determine if piscidin expression could be modulated by diet, greater amberjack were fed diets supplemented with MOS and cMOS for 30 days when transcript level was determined. It was found that MOS supplemented diets increased expression in the spleen, cMOS supplemented diets upregulated transcript levels in the gills and head kidney, whilst a diet containing both MOS and cMOS upregulated transcript in the gut, when compared to fish fed the control diet. Finally, a synthetic greater amberjack piscidin was produced and showed bacteriostatic activity against a number of bacterial strains, including both Gram positive and Gram negative fish pathogens.

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