Tsetse flies serve as biological vectors for several species of African trypanosomes. In order to survive, proliferate and establish a midgut infection, trypanosomes must cross the tsetse fly peritrophic matrix (PM), which is an acellular gut lining surrounding the blood meal. Crossing of this multi-layered structure occurs at least twice during parasite migration and development, but the mechanism of how trypanosomes do so is not understood. In order to better comprehend the molecular events surrounding trypanosome penetration of the tsetse PM, a mass spectrometry-based approach was applied to investigate the PM protein composition using Glossina morsitans morsitans as a model organism.
Rose, C., Belmonte, R., Armstrong, S. D., Molyneux, G., Haines, L. R., Lehane, M. J., ... Acosta-Serrano, A. (2014). An Investigation into the protein composition of the teneral Glossina morsitans morsitans peritrophic matrix. PLoS Neglected Tropical Diseases, 8(4), [e2691]. https://doi.org/10.1371/journal.pntd.0002691