Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica)

Pietro Tedesco, Isabel Maida, Fortunato Palma Esposito, Emiliana Tortorella, Karolina Subko, Chidinma Christiana Ezeofor, Ying Zhang, Jioji Tabudravu, Marcel Jaspars, Renato Fani, Donatella de Pascale

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Abstract

Microorganisms living in extreme environments represent a huge reservoir of novel antimicrobial compounds and possibly of novel chemical families. Antarctica is one of the most extraordinary places on Earth and exhibits many distinctive features. Antarctic microorganisms are well known producers of valuable secondary metabolites. Specifically, several Antarctic strains have been reported to inhibit opportunistic human pathogens strains belonging to Burkholderia cepacia complex (Bcc). Herein, we applied a biodiscovery pipeline for the identification of anti-Bcc compounds. Antarctic sub-sea sediments were collected from the Ross Sea, and used to isolate 25 microorganisms, which were phylogenetically affiliated to three bacterial genera (Psychrobacter, Arthrobacter, and Pseudomonas) via sequencing and analysis of 16S rRNA genes. They were then subjected to a primary cell-based screening to determine their bioactivity against Bcc strains. Positive isolates were used to produce crude extracts from microbial spent culture media, to perform the secondary screening. Strain Pseudomonas BNT1 was then selected for bioassay-guided purification employing SPE and HPLC. Finally, LC-MS and NMR structurally resolved the purified bioactive compounds. With this strategy, we achieved the isolation of three rhamnolipids, two of which were new, endowed with high (MIC < 1 μg/mL) and unreported antimicrobial activity against Bcc strains.

Original languageEnglish
Article number83
Pages (from-to)1-14
Number of pages14
JournalMarine Drugs
Volume14
Issue number5
DOIs
Publication statusPublished - 26 Apr 2016

Fingerprint

Burkholderia cepacia complex
Oceans and Seas
Pseudomonas
Psychrobacter
Arthrobacter
Complex Mixtures
rRNA Genes
Biological Assay
Culture Media
High Pressure Liquid Chromatography

Keywords

  • antimicrobials
  • ramnholipids
  • Antarctic
  • Bcc
  • microorganisms

Cite this

Tedesco, P., Maida, I., Palma Esposito, F., Tortorella, E., Subko, K., Ezeofor, C. C., ... de Pascale, D. (2016). Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica). Marine Drugs, 14(5), 1-14. [83]. https://doi.org/10.3390/md14050083

Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica). / Tedesco, Pietro; Maida, Isabel; Palma Esposito, Fortunato; Tortorella, Emiliana; Subko, Karolina; Ezeofor, Chidinma Christiana; Zhang, Ying; Tabudravu, Jioji; Jaspars, Marcel; Fani, Renato; de Pascale, Donatella.

In: Marine Drugs, Vol. 14, No. 5, 83, 26.04.2016, p. 1-14.

Research output: Contribution to journalArticle

Tedesco, P, Maida, I, Palma Esposito, F, Tortorella, E, Subko, K, Ezeofor, CC, Zhang, Y, Tabudravu, J, Jaspars, M, Fani, R & de Pascale, D 2016, 'Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica)', Marine Drugs, vol. 14, no. 5, 83, pp. 1-14. https://doi.org/10.3390/md14050083
Tedesco P, Maida I, Palma Esposito F, Tortorella E, Subko K, Ezeofor CC et al. Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica). Marine Drugs. 2016 Apr 26;14(5):1-14. 83. https://doi.org/10.3390/md14050083
Tedesco, Pietro ; Maida, Isabel ; Palma Esposito, Fortunato ; Tortorella, Emiliana ; Subko, Karolina ; Ezeofor, Chidinma Christiana ; Zhang, Ying ; Tabudravu, Jioji ; Jaspars, Marcel ; Fani, Renato ; de Pascale, Donatella. / Antimicrobial Activity of Monoramnholipids Produced by Bacterial Strains Isolated from the Ross Sea (Antarctica). In: Marine Drugs. 2016 ; Vol. 14, No. 5. pp. 1-14.
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N2 - Microorganisms living in extreme environments represent a huge reservoir of novel antimicrobial compounds and possibly of novel chemical families. Antarctica is one of the most extraordinary places on Earth and exhibits many distinctive features. Antarctic microorganisms are well known producers of valuable secondary metabolites. Specifically, several Antarctic strains have been reported to inhibit opportunistic human pathogens strains belonging to Burkholderia cepacia complex (Bcc). Herein, we applied a biodiscovery pipeline for the identification of anti-Bcc compounds. Antarctic sub-sea sediments were collected from the Ross Sea, and used to isolate 25 microorganisms, which were phylogenetically affiliated to three bacterial genera (Psychrobacter, Arthrobacter, and Pseudomonas) via sequencing and analysis of 16S rRNA genes. They were then subjected to a primary cell-based screening to determine their bioactivity against Bcc strains. Positive isolates were used to produce crude extracts from microbial spent culture media, to perform the secondary screening. Strain Pseudomonas BNT1 was then selected for bioassay-guided purification employing SPE and HPLC. Finally, LC-MS and NMR structurally resolved the purified bioactive compounds. With this strategy, we achieved the isolation of three rhamnolipids, two of which were new, endowed with high (MIC < 1 μg/mL) and unreported antimicrobial activity against Bcc strains.

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