Arsenolipids show different profiles in muscle tissues of four commercial fish species

Kenneth O. Amayo, Andrea Raab, Eva M. Krupp, Talke Marschall, Michael J. Horsfall, Jörg Feldmann

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Identification of arsenolipids in biological samples is today a challenge and in particular the need for speciation data for toxicological assessment. Fish is one of the major contributors of arsenic in diet. However, the majority of work in this area has only focused on the water soluble compounds. The aim of this study is to provide some data on total arsenic and in particular to gain insights into the types of arsenolipids in the muscle tissues of four commercial and commonly consumed fish species. Determination of total arsenic was carried out by ICP-MS following microwave-assisted acid digestion of the samples and the concentrations found for total arsenic in the muscles ranged from 4.8 to 6.0. μg/g. d.w. Sequential extraction was carried out using hexane and MeOH/DCM followed by reversed phase HPLC-ICP-MS/ESI-MS analysis of the MeOH/DCM fraction. Eight arsenolipids including three arsenic fatty acids (AsFAs) and five arsenic hydrocarbons (AsHCs) were identified. The result showed that fish with higher arsenolipid (AsLp) content (brill and sardine) are dominated by AsHC, while those with the smaller proportion of AsLp (mackerel and red mullet) have predominately arsenic in the form of AsFA.
Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalJournal of Trace Elements in Medicine and Biology
Volume28
Issue number2
Early online date23 Nov 2013
DOIs
Publication statusPublished - Apr 2014

Fingerprint

Arsenic
Fish
Muscle
Fishes
Tissue
Muscles
Hydrocarbons
Fatty Acids
Smegmamorpha
Perciformes
Hexanes
Microwaves
Toxicology
Digestion
Nutrition
High Pressure Liquid Chromatography
Diet
Acids
Water

Keywords

  • Arsenolipids
  • ESI-Orbitrap-MS
  • Fish muscles
  • ICP-MS
  • RP-HPLC

Cite this

Arsenolipids show different profiles in muscle tissues of four commercial fish species. / Amayo, Kenneth O.; Raab, Andrea; Krupp, Eva M.; Marschall, Talke; Horsfall, Michael J.; Feldmann, Jörg.

In: Journal of Trace Elements in Medicine and Biology, Vol. 28, No. 2, 04.2014, p. 131-137.

Research output: Contribution to journalArticle

@article{9a4f9b8074bc4a5991319122daf56653,
title = "Arsenolipids show different profiles in muscle tissues of four commercial fish species",
abstract = "Identification of arsenolipids in biological samples is today a challenge and in particular the need for speciation data for toxicological assessment. Fish is one of the major contributors of arsenic in diet. However, the majority of work in this area has only focused on the water soluble compounds. The aim of this study is to provide some data on total arsenic and in particular to gain insights into the types of arsenolipids in the muscle tissues of four commercial and commonly consumed fish species. Determination of total arsenic was carried out by ICP-MS following microwave-assisted acid digestion of the samples and the concentrations found for total arsenic in the muscles ranged from 4.8 to 6.0. μg/g. d.w. Sequential extraction was carried out using hexane and MeOH/DCM followed by reversed phase HPLC-ICP-MS/ESI-MS analysis of the MeOH/DCM fraction. Eight arsenolipids including three arsenic fatty acids (AsFAs) and five arsenic hydrocarbons (AsHCs) were identified. The result showed that fish with higher arsenolipid (AsLp) content (brill and sardine) are dominated by AsHC, while those with the smaller proportion of AsLp (mackerel and red mullet) have predominately arsenic in the form of AsFA.",
keywords = "Arsenolipids, ESI-Orbitrap-MS, Fish muscles, ICP-MS, RP-HPLC",
author = "Amayo, {Kenneth O.} and Andrea Raab and Krupp, {Eva M.} and Talke Marschall and Horsfall, {Michael J.} and J{\"o}rg Feldmann",
note = "K.O.A. thanks the Ambrose Alli University in collaboration with the Education Trust Fund, Nigeria for financial support. TESLA research funds supported the work financially. M.H.Jr. thanks the Royal Society of Chemistry for financial support to spend time at University of Aberdee",
year = "2014",
month = "4",
doi = "10.1016/j.jtemb.2013.11.004",
language = "English",
volume = "28",
pages = "131--137",
journal = "Journal of Trace Elements in Medicine and Biology",
issn = "0946-672X",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "2",

}

TY - JOUR

T1 - Arsenolipids show different profiles in muscle tissues of four commercial fish species

AU - Amayo, Kenneth O.

AU - Raab, Andrea

AU - Krupp, Eva M.

AU - Marschall, Talke

AU - Horsfall, Michael J.

AU - Feldmann, Jörg

N1 - K.O.A. thanks the Ambrose Alli University in collaboration with the Education Trust Fund, Nigeria for financial support. TESLA research funds supported the work financially. M.H.Jr. thanks the Royal Society of Chemistry for financial support to spend time at University of Aberdee

PY - 2014/4

Y1 - 2014/4

N2 - Identification of arsenolipids in biological samples is today a challenge and in particular the need for speciation data for toxicological assessment. Fish is one of the major contributors of arsenic in diet. However, the majority of work in this area has only focused on the water soluble compounds. The aim of this study is to provide some data on total arsenic and in particular to gain insights into the types of arsenolipids in the muscle tissues of four commercial and commonly consumed fish species. Determination of total arsenic was carried out by ICP-MS following microwave-assisted acid digestion of the samples and the concentrations found for total arsenic in the muscles ranged from 4.8 to 6.0. μg/g. d.w. Sequential extraction was carried out using hexane and MeOH/DCM followed by reversed phase HPLC-ICP-MS/ESI-MS analysis of the MeOH/DCM fraction. Eight arsenolipids including three arsenic fatty acids (AsFAs) and five arsenic hydrocarbons (AsHCs) were identified. The result showed that fish with higher arsenolipid (AsLp) content (brill and sardine) are dominated by AsHC, while those with the smaller proportion of AsLp (mackerel and red mullet) have predominately arsenic in the form of AsFA.

AB - Identification of arsenolipids in biological samples is today a challenge and in particular the need for speciation data for toxicological assessment. Fish is one of the major contributors of arsenic in diet. However, the majority of work in this area has only focused on the water soluble compounds. The aim of this study is to provide some data on total arsenic and in particular to gain insights into the types of arsenolipids in the muscle tissues of four commercial and commonly consumed fish species. Determination of total arsenic was carried out by ICP-MS following microwave-assisted acid digestion of the samples and the concentrations found for total arsenic in the muscles ranged from 4.8 to 6.0. μg/g. d.w. Sequential extraction was carried out using hexane and MeOH/DCM followed by reversed phase HPLC-ICP-MS/ESI-MS analysis of the MeOH/DCM fraction. Eight arsenolipids including three arsenic fatty acids (AsFAs) and five arsenic hydrocarbons (AsHCs) were identified. The result showed that fish with higher arsenolipid (AsLp) content (brill and sardine) are dominated by AsHC, while those with the smaller proportion of AsLp (mackerel and red mullet) have predominately arsenic in the form of AsFA.

KW - Arsenolipids

KW - ESI-Orbitrap-MS

KW - Fish muscles

KW - ICP-MS

KW - RP-HPLC

UR - http://www.scopus.com/inward/record.url?scp=84898052585&partnerID=8YFLogxK

U2 - 10.1016/j.jtemb.2013.11.004

DO - 10.1016/j.jtemb.2013.11.004

M3 - Article

VL - 28

SP - 131

EP - 137

JO - Journal of Trace Elements in Medicine and Biology

JF - Journal of Trace Elements in Medicine and Biology

SN - 0946-672X

IS - 2

ER -