Assessment of immuno stimulants as Mx inducers in Atlantic salmon (Salmo salar L.) parr and the effect of temperature on the kinetics of Mx responses

I Salinas, K Lockhart, T J Bowden, B Collet, C Secombes, A E Ellis

Research output: Contribution to journalArticle

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Abstract

The aims of this study were (i) to identify alternative Mx stimulatory compounds in Atlantic salmon (Salmo salar L.) and to characterise the kinetics and intensity of the stimulated responses and (ii) to investigate the effect of temperature on such responses by semi-quantitative RT-PCR. Mx transcripts were measured in Atlantic salmon parr kept at 14 degreesC and injected with either LPS, the synthetic double-stranded polyribonucleotide poly I:C, Vibrio anguillarum serotypes I and II-ordalii bacterin, beta-glucan, whole yeast cells or yeast RNA. Sampling periods lasted until transcripts were undetectable or up to three weeks after immunisation. The effect of temperature on poly 1:C-induced Mx response was studied by injecting parr kept at 6 degreesC. Newly hatched salmon fry were immersed once, twice or three times in the Vibrio bacterin diluted five or 10 times and sampled for three weeks.

None of the yeast compounds induced Mx expression in Atlantic salmon parr. LPS induced a very low Mx response 2 and 3 days after injection. The Vibrio bacterin administered by injection in parr (but not by immersion in fry) resulted in strong Mx induction on days 2 and 3, disappearing by day 6. Poly I:C-induced Mx responses that were more intense and longer lasting than those induced by the bacterin, peaking on day 3 and lasting over 6 days, disappearing by day 9 at 14 degreesC. Lower temperature caused a longer lasting Mx response to poly I:C (at least 21 days), which peaked on days 7-14, with a similar intensity and no delayed onset as compared with the response at 14 degreesC. However, some toxicity of the poly I:C was indicated in treatments at 6 degreesC. (C) 2004 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)159-170
Number of pages12
JournalFish & Shellfish Immunology
Volume17
DOIs
Publication statusPublished - 2004

Keywords

  • Mx responses
  • immunostimulants
  • Atlantic salmon
  • type I interferon
  • poly I : C
  • Vibrio bacterin
  • LPS
  • glucan
  • yeast cells
  • yeast RNA
  • temperature effect
  • DOUBLE-STRANDED-RNA
  • POLY I-C
  • HEMATOPOIETIC NECROSIS VIRUS
  • RAINBOW-TROUT
  • ONCORHYNCHUS-MYKISS
  • ANTIVIRAL ACTIVITY
  • MOLECULAR-CLONING
  • RESPIRATORY BURST
  • L MACROPHAGES
  • YEAST GLUCAN

Cite this

Assessment of immuno stimulants as Mx inducers in Atlantic salmon (Salmo salar L.) parr and the effect of temperature on the kinetics of Mx responses. / Salinas, I ; Lockhart, K ; Bowden, T J ; Collet, B ; Secombes, C ; Ellis, A E .

In: Fish & Shellfish Immunology, Vol. 17, 2004, p. 159-170.

Research output: Contribution to journalArticle

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T1 - Assessment of immuno stimulants as Mx inducers in Atlantic salmon (Salmo salar L.) parr and the effect of temperature on the kinetics of Mx responses

AU - Salinas, I

AU - Lockhart, K

AU - Bowden, T J

AU - Collet, B

AU - Secombes, C

AU - Ellis, A E

PY - 2004

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N2 - The aims of this study were (i) to identify alternative Mx stimulatory compounds in Atlantic salmon (Salmo salar L.) and to characterise the kinetics and intensity of the stimulated responses and (ii) to investigate the effect of temperature on such responses by semi-quantitative RT-PCR. Mx transcripts were measured in Atlantic salmon parr kept at 14 degreesC and injected with either LPS, the synthetic double-stranded polyribonucleotide poly I:C, Vibrio anguillarum serotypes I and II-ordalii bacterin, beta-glucan, whole yeast cells or yeast RNA. Sampling periods lasted until transcripts were undetectable or up to three weeks after immunisation. The effect of temperature on poly 1:C-induced Mx response was studied by injecting parr kept at 6 degreesC. Newly hatched salmon fry were immersed once, twice or three times in the Vibrio bacterin diluted five or 10 times and sampled for three weeks.None of the yeast compounds induced Mx expression in Atlantic salmon parr. LPS induced a very low Mx response 2 and 3 days after injection. The Vibrio bacterin administered by injection in parr (but not by immersion in fry) resulted in strong Mx induction on days 2 and 3, disappearing by day 6. Poly I:C-induced Mx responses that were more intense and longer lasting than those induced by the bacterin, peaking on day 3 and lasting over 6 days, disappearing by day 9 at 14 degreesC. Lower temperature caused a longer lasting Mx response to poly I:C (at least 21 days), which peaked on days 7-14, with a similar intensity and no delayed onset as compared with the response at 14 degreesC. However, some toxicity of the poly I:C was indicated in treatments at 6 degreesC. (C) 2004 Elsevier Ltd. All rights reserved.

AB - The aims of this study were (i) to identify alternative Mx stimulatory compounds in Atlantic salmon (Salmo salar L.) and to characterise the kinetics and intensity of the stimulated responses and (ii) to investigate the effect of temperature on such responses by semi-quantitative RT-PCR. Mx transcripts were measured in Atlantic salmon parr kept at 14 degreesC and injected with either LPS, the synthetic double-stranded polyribonucleotide poly I:C, Vibrio anguillarum serotypes I and II-ordalii bacterin, beta-glucan, whole yeast cells or yeast RNA. Sampling periods lasted until transcripts were undetectable or up to three weeks after immunisation. The effect of temperature on poly 1:C-induced Mx response was studied by injecting parr kept at 6 degreesC. Newly hatched salmon fry were immersed once, twice or three times in the Vibrio bacterin diluted five or 10 times and sampled for three weeks.None of the yeast compounds induced Mx expression in Atlantic salmon parr. LPS induced a very low Mx response 2 and 3 days after injection. The Vibrio bacterin administered by injection in parr (but not by immersion in fry) resulted in strong Mx induction on days 2 and 3, disappearing by day 6. Poly I:C-induced Mx responses that were more intense and longer lasting than those induced by the bacterin, peaking on day 3 and lasting over 6 days, disappearing by day 9 at 14 degreesC. Lower temperature caused a longer lasting Mx response to poly I:C (at least 21 days), which peaked on days 7-14, with a similar intensity and no delayed onset as compared with the response at 14 degreesC. However, some toxicity of the poly I:C was indicated in treatments at 6 degreesC. (C) 2004 Elsevier Ltd. All rights reserved.

KW - Mx responses

KW - immunostimulants

KW - Atlantic salmon

KW - type I interferon

KW - poly I : C

KW - Vibrio bacterin

KW - LPS

KW - glucan

KW - yeast cells

KW - yeast RNA

KW - temperature effect

KW - DOUBLE-STRANDED-RNA

KW - POLY I-C

KW - HEMATOPOIETIC NECROSIS VIRUS

KW - RAINBOW-TROUT

KW - ONCORHYNCHUS-MYKISS

KW - ANTIVIRAL ACTIVITY

KW - MOLECULAR-CLONING

KW - RESPIRATORY BURST

KW - L MACROPHAGES

KW - YEAST GLUCAN

U2 - 10.1016/j.fsi.2004.01.003

DO - 10.1016/j.fsi.2004.01.003

M3 - Article

VL - 17

SP - 159

EP - 170

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

ER -