Assessment of plants from the Brassicaceae family as genetic models for the study of nickel and zinc hyperaccumulation

Wendy Ann Peer, Mehrzad Mahmoudian, John L. Freeman, Brett Lahner, Elizabeth L. Richards, Roger D. Reeves, Angus S. Murphy, David E. Salt

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We report on the second phase of a programme to select a relative of Arabidopsis thaliana for use in large-scale molecular genetic studies of nickel (Ni) and zinc (Zn) hyperaccumulation. We also report on the relatedness among Thlaspi caerulescens accessions and the utility of using O-acetyl-L-serine as a marker for Ni and Zn hyperaccumulation potential.

Twenty-seven new accessions of metal-accumulating species collected in the Czech Republic, France, Greece, Italy, Slovenia and the USA during Spring-Summer 2002 were evaluated.

The criteria established for selection were hyperaccumulation of metals (Ni and Zn); compact growth habit; reasonable time to flowering; production of >= 1000 seeds per plant; self-fertility; compact diploid genome; high sequence similarity to A. thaliana; >= 0.1% transformation efficiency with easy selection.

We conclude that the best candidate identified in the first phase was the best candidate overall: T. caerulescens accession St Felix de Pallieres.

Original languageEnglish
Pages (from-to)248-260
Number of pages13
JournalNew Phytologist
Volume172
Issue number2
DOIs
Publication statusPublished - 2006

Keywords

  • Arabidopsis
  • Bornmuellera
  • genome size
  • metal hyperaccumulation
  • model system
  • Peltaria
  • Thlaspi
  • transformation
  • thlaspi-caerulescens
  • arabidopsis-halleri
  • transporter genes
  • accumulation
  • sequences
  • phytoremediation
  • accessions
  • tolerance
  • alignment

Cite this

Assessment of plants from the Brassicaceae family as genetic models for the study of nickel and zinc hyperaccumulation. / Peer, Wendy Ann; Mahmoudian, Mehrzad; Freeman, John L.; Lahner, Brett; Richards, Elizabeth L.; Reeves, Roger D.; Murphy, Angus S.; Salt, David E.

In: New Phytologist, Vol. 172, No. 2, 2006, p. 248-260.

Research output: Contribution to journalArticle

Peer, WA, Mahmoudian, M, Freeman, JL, Lahner, B, Richards, EL, Reeves, RD, Murphy, AS & Salt, DE 2006, 'Assessment of plants from the Brassicaceae family as genetic models for the study of nickel and zinc hyperaccumulation', New Phytologist, vol. 172, no. 2, pp. 248-260. https://doi.org/10.1111/j.1469-8137.2006.01820.x
Peer, Wendy Ann ; Mahmoudian, Mehrzad ; Freeman, John L. ; Lahner, Brett ; Richards, Elizabeth L. ; Reeves, Roger D. ; Murphy, Angus S. ; Salt, David E. / Assessment of plants from the Brassicaceae family as genetic models for the study of nickel and zinc hyperaccumulation. In: New Phytologist. 2006 ; Vol. 172, No. 2. pp. 248-260.
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