Augmentation of killing of Escherichia coli O157 by combinations of lactate, ethanol, and low-pH conditions

S L Jordan, J Glover, L Malcolm, F M Thomson-Carter, I R Booth, S F Park

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The acid tolerance of Escherichia coli O157:H7 strains can be overcome by addition of lactate, ethanol, or a combination of the two agents. Killing can be increased by as much as 4 log units in the first 5 min of incubation at pH 3 even for the most acid-tolerant isolates. Exponential-phase, habituated, and stationary-phase cells are all sensitive to incubation with lactate and ethanol. Killing correlates with disruption of the capacity for pH homeostasis. Habituated and, stationary-phase cells can partially offset the effects of the lowering of cytoplasmic pH.

Original languageEnglish
Pages (from-to)1308-1311
Number of pages4
JournalApplied and Environmental Microbiology
Volume65
Publication statusPublished - 1999

Keywords

  • ACID TOLERANCE RESPONSE
  • MONOCYTOGENES SCOTT-A
  • LISTERIA-MONOCYTOGENES
  • SALMONELLA-TYPHIMURIUM
  • INTRACELLULAR PH
  • APPLE CIDER
  • SURVIVAL
  • GROWTH
  • HABITUATION
  • RESISTANCE

Cite this

Jordan, S. L., Glover, J., Malcolm, L., Thomson-Carter, F. M., Booth, I. R., & Park, S. F. (1999). Augmentation of killing of Escherichia coli O157 by combinations of lactate, ethanol, and low-pH conditions. Applied and Environmental Microbiology, 65, 1308-1311.

Augmentation of killing of Escherichia coli O157 by combinations of lactate, ethanol, and low-pH conditions. / Jordan, S L ; Glover, J ; Malcolm, L ; Thomson-Carter, F M ; Booth, I R ; Park, S F .

In: Applied and Environmental Microbiology, Vol. 65, 1999, p. 1308-1311.

Research output: Contribution to journalArticle

Jordan, SL, Glover, J, Malcolm, L, Thomson-Carter, FM, Booth, IR & Park, SF 1999, 'Augmentation of killing of Escherichia coli O157 by combinations of lactate, ethanol, and low-pH conditions', Applied and Environmental Microbiology, vol. 65, pp. 1308-1311.
Jordan, S L ; Glover, J ; Malcolm, L ; Thomson-Carter, F M ; Booth, I R ; Park, S F . / Augmentation of killing of Escherichia coli O157 by combinations of lactate, ethanol, and low-pH conditions. In: Applied and Environmental Microbiology. 1999 ; Vol. 65. pp. 1308-1311.
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AU - Booth, I R

AU - Park, S F

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AB - The acid tolerance of Escherichia coli O157:H7 strains can be overcome by addition of lactate, ethanol, or a combination of the two agents. Killing can be increased by as much as 4 log units in the first 5 min of incubation at pH 3 even for the most acid-tolerant isolates. Exponential-phase, habituated, and stationary-phase cells are all sensitive to incubation with lactate and ethanol. Killing correlates with disruption of the capacity for pH homeostasis. Habituated and, stationary-phase cells can partially offset the effects of the lowering of cytoplasmic pH.

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KW - INTRACELLULAR PH

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