The acid tolerance of Escherichia coli O157:H7 strains can be overcome by addition of lactate, ethanol, or a combination of the two agents. Killing can be increased by as much as 4 log units in the first 5 min of incubation at pH 3 even for the most acid-tolerant isolates. Exponential-phase, habituated, and stationary-phase cells are all sensitive to incubation with lactate and ethanol. Killing correlates with disruption of the capacity for pH homeostasis. Habituated and, stationary-phase cells can partially offset the effects of the lowering of cytoplasmic pH.
|Number of pages||4|
|Journal||Applied and Environmental Microbiology|
|Publication status||Published - 1999|
- ACID TOLERANCE RESPONSE
- MONOCYTOGENES SCOTT-A
- INTRACELLULAR PH
- APPLE CIDER