Biological sulphur-containing compounds – Analytical challenges

Andrea Raab* (Corresponding Author), Jörg Feldmann

*Corresponding author for this work

Research output: Contribution to journalReview article

Abstract

This review covers analytical methods applied to the determination of none volatile sulphur-containing biological compounds. The classes of S-compounds include amino acids, proteins, lipids, carbohydrates and sulphur-containing metabolites. Techniques covered include element specific detectors as well as molecular specific detectors from X-ray absorption near-edge structure (XANES) to elemental and molecular mass spectrometers. The major techniques used are inductively coupled plasma mass spectrometry (ICP-MS) and high-resolution electrospray mass spectrometry (ESI-MS) in their various forms. Both techniques either individually or combined require the sample to be present in liquid form and therefore involve sample preparation usually extraction and depending on sample and molecular class studied potentially also derivatisation in addition to generally requiring chromatographic separation. Over recent years, detection limits achieved by elemental methods and computational methods to extract signals of sulphur-containing compounds out of the mass of data produced by molecular high-resolution mass spectrometers made significant gains. Still the determination of sulphur-containing compounds is challenging, but nowadays the methods have been developed well enough to allow application to real samples for absolute quantification of biomolecules such as proteins or lipids.

Original languageEnglish
Pages (from-to)20-29
Number of pages10
JournalAnalytica Chimica Acta
Volume1079
Early online date29 May 2019
DOIs
Publication statusPublished - 4 Nov 2019

Fingerprint

Sulfur Compounds
sulfur compound
Sulfur
Mass spectrometers
spectrometer
mass spectrometry
lipid
sulfur
protein
sample preparation
Detectors
Sulfur Amino Acids
Lipids
Inductively coupled plasma mass spectrometry
Electrospray Ionization Mass Spectrometry
carbohydrate
analytical method
metabolite
X ray absorption
Molecular mass

Keywords

  • Detection
  • Review
  • Sample preparation
  • Separation
  • Sulfur
  • Sulfur-containing compound
  • CAPILLARY LIQUID-CHROMATOGRAPHY
  • ALLIUM-SATIVUM L.
  • ISOTOPE-DILUTION ANALYSIS
  • CONTAINING AMINO-ACIDS
  • ORGANOSULFUR COMPOUNDS
  • PLASMA-MASS SPECTROMETRY
  • CONTAINING METABOLITES
  • ICP-MS
  • AFFINITY-CHROMATOGRAPHY
  • ABSOLUTE QUANTIFICATION

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Biological sulphur-containing compounds – Analytical challenges. / Raab, Andrea (Corresponding Author); Feldmann, Jörg.

In: Analytica Chimica Acta, Vol. 1079, 04.11.2019, p. 20-29.

Research output: Contribution to journalReview article

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abstract = "This review covers analytical methods applied to the determination of none volatile sulphur-containing biological compounds. The classes of S-compounds include amino acids, proteins, lipids, carbohydrates and sulphur-containing metabolites. Techniques covered include element specific detectors as well as molecular specific detectors from X-ray absorption near-edge structure (XANES) to elemental and molecular mass spectrometers. The major techniques used are inductively coupled plasma mass spectrometry (ICP-MS) and high-resolution electrospray mass spectrometry (ESI-MS) in their various forms. Both techniques either individually or combined require the sample to be present in liquid form and therefore involve sample preparation usually extraction and depending on sample and molecular class studied potentially also derivatisation in addition to generally requiring chromatographic separation. Over recent years, detection limits achieved by elemental methods and computational methods to extract signals of sulphur-containing compounds out of the mass of data produced by molecular high-resolution mass spectrometers made significant gains. Still the determination of sulphur-containing compounds is challenging, but nowadays the methods have been developed well enough to allow application to real samples for absolute quantification of biomolecules such as proteins or lipids.",
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