Campylobacter immunity and coinfection following a large outbreak in a farming community

K. J. Forbes, F. J. Gormley, J. F. Dallas, O. Labovitiadi, M. MacRae, R. J. Owen, J. Richardson, N. J. C. Strachan, J. M. Cowden, I. D. Ogden, C. C. McGuigan

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

An outbreak of campylobacteriosis affected approximately one-half of 165 people attending an annual farmers' dance in Montrose, Scotland, in November 2005. Epidemiological investigations, including a cohort study (n = 164), identified chicken liver pate as the most likely vehicle of infection. Pate preparation involved deliberate undercooking of chicken livers by flash-frying, followed by mechanical homogenization. Typing of 32 Campylobacter strains ( isolated from submitted stools) by multilocus sequence typing identified four distinct clades of Campylobacter jejuni. There was good agreement when isolates were typed by Penner serotyping, pulsed-field gel electrophoresis, and flaA short variable region sequencing but poorer agreement with phage and antibiotic susceptibility testing. At least three attendees were coinfected with two Campylobacter strains each. The outbreak was probably due to several livers contributing Campylobacter strains that survived undercooking and were dispersed throughout the pate. The study highlights improper culinary procedures as a potential human health risk and provides a striking counterexample to the "dominant outbreak strain" view of point source outbreaks of food-borne infections. It also demonstrates that previous exposure to biologically plausible sources of Campylobacter may confer protection against subsequent infection.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalJournal of Clinical Microbiology
Volume47
Issue number1
Early online date12 Nov 2008
DOIs
Publication statusPublished - Jan 2009

Keywords

  • sequence typing system
  • infectious-disease
  • Jejuni infection
  • public-health
  • coli
  • England
  • poultry
  • Wales
  • food
  • contamination

Cite this

Forbes, K. J., Gormley, F. J., Dallas, J. F., Labovitiadi, O., MacRae, M., Owen, R. J., ... McGuigan, C. C. (2009). Campylobacter immunity and coinfection following a large outbreak in a farming community. Journal of Clinical Microbiology, 47(1), 111-116. https://doi.org/10.1128/JCM.01731-08

Campylobacter immunity and coinfection following a large outbreak in a farming community. / Forbes, K. J.; Gormley, F. J.; Dallas, J. F.; Labovitiadi, O.; MacRae, M.; Owen, R. J.; Richardson, J.; Strachan, N. J. C.; Cowden, J. M.; Ogden, I. D.; McGuigan, C. C.

In: Journal of Clinical Microbiology, Vol. 47, No. 1, 01.2009, p. 111-116.

Research output: Contribution to journalArticle

Forbes, KJ, Gormley, FJ, Dallas, JF, Labovitiadi, O, MacRae, M, Owen, RJ, Richardson, J, Strachan, NJC, Cowden, JM, Ogden, ID & McGuigan, CC 2009, 'Campylobacter immunity and coinfection following a large outbreak in a farming community', Journal of Clinical Microbiology, vol. 47, no. 1, pp. 111-116. https://doi.org/10.1128/JCM.01731-08
Forbes, K. J. ; Gormley, F. J. ; Dallas, J. F. ; Labovitiadi, O. ; MacRae, M. ; Owen, R. J. ; Richardson, J. ; Strachan, N. J. C. ; Cowden, J. M. ; Ogden, I. D. ; McGuigan, C. C. / Campylobacter immunity and coinfection following a large outbreak in a farming community. In: Journal of Clinical Microbiology. 2009 ; Vol. 47, No. 1. pp. 111-116.
@article{830dfb7384004c1dadd5241328c6a41a,
title = "Campylobacter immunity and coinfection following a large outbreak in a farming community",
abstract = "An outbreak of campylobacteriosis affected approximately one-half of 165 people attending an annual farmers' dance in Montrose, Scotland, in November 2005. Epidemiological investigations, including a cohort study (n = 164), identified chicken liver pate as the most likely vehicle of infection. Pate preparation involved deliberate undercooking of chicken livers by flash-frying, followed by mechanical homogenization. Typing of 32 Campylobacter strains ( isolated from submitted stools) by multilocus sequence typing identified four distinct clades of Campylobacter jejuni. There was good agreement when isolates were typed by Penner serotyping, pulsed-field gel electrophoresis, and flaA short variable region sequencing but poorer agreement with phage and antibiotic susceptibility testing. At least three attendees were coinfected with two Campylobacter strains each. The outbreak was probably due to several livers contributing Campylobacter strains that survived undercooking and were dispersed throughout the pate. The study highlights improper culinary procedures as a potential human health risk and provides a striking counterexample to the {"}dominant outbreak strain{"} view of point source outbreaks of food-borne infections. It also demonstrates that previous exposure to biologically plausible sources of Campylobacter may confer protection against subsequent infection.",
keywords = "sequence typing system, infectious-disease, Jejuni infection, public-health, coli, England, poultry, Wales, food, contamination",
author = "Forbes, {K. J.} and Gormley, {F. J.} and Dallas, {J. F.} and O. Labovitiadi and M. MacRae and Owen, {R. J.} and J. Richardson and Strachan, {N. J. C.} and Cowden, {J. M.} and Ogden, {I. D.} and McGuigan, {C. C.}",
year = "2009",
month = "1",
doi = "10.1128/JCM.01731-08",
language = "English",
volume = "47",
pages = "111--116",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Campylobacter immunity and coinfection following a large outbreak in a farming community

AU - Forbes, K. J.

AU - Gormley, F. J.

AU - Dallas, J. F.

AU - Labovitiadi, O.

AU - MacRae, M.

AU - Owen, R. J.

AU - Richardson, J.

AU - Strachan, N. J. C.

AU - Cowden, J. M.

AU - Ogden, I. D.

AU - McGuigan, C. C.

PY - 2009/1

Y1 - 2009/1

N2 - An outbreak of campylobacteriosis affected approximately one-half of 165 people attending an annual farmers' dance in Montrose, Scotland, in November 2005. Epidemiological investigations, including a cohort study (n = 164), identified chicken liver pate as the most likely vehicle of infection. Pate preparation involved deliberate undercooking of chicken livers by flash-frying, followed by mechanical homogenization. Typing of 32 Campylobacter strains ( isolated from submitted stools) by multilocus sequence typing identified four distinct clades of Campylobacter jejuni. There was good agreement when isolates were typed by Penner serotyping, pulsed-field gel electrophoresis, and flaA short variable region sequencing but poorer agreement with phage and antibiotic susceptibility testing. At least three attendees were coinfected with two Campylobacter strains each. The outbreak was probably due to several livers contributing Campylobacter strains that survived undercooking and were dispersed throughout the pate. The study highlights improper culinary procedures as a potential human health risk and provides a striking counterexample to the "dominant outbreak strain" view of point source outbreaks of food-borne infections. It also demonstrates that previous exposure to biologically plausible sources of Campylobacter may confer protection against subsequent infection.

AB - An outbreak of campylobacteriosis affected approximately one-half of 165 people attending an annual farmers' dance in Montrose, Scotland, in November 2005. Epidemiological investigations, including a cohort study (n = 164), identified chicken liver pate as the most likely vehicle of infection. Pate preparation involved deliberate undercooking of chicken livers by flash-frying, followed by mechanical homogenization. Typing of 32 Campylobacter strains ( isolated from submitted stools) by multilocus sequence typing identified four distinct clades of Campylobacter jejuni. There was good agreement when isolates were typed by Penner serotyping, pulsed-field gel electrophoresis, and flaA short variable region sequencing but poorer agreement with phage and antibiotic susceptibility testing. At least three attendees were coinfected with two Campylobacter strains each. The outbreak was probably due to several livers contributing Campylobacter strains that survived undercooking and were dispersed throughout the pate. The study highlights improper culinary procedures as a potential human health risk and provides a striking counterexample to the "dominant outbreak strain" view of point source outbreaks of food-borne infections. It also demonstrates that previous exposure to biologically plausible sources of Campylobacter may confer protection against subsequent infection.

KW - sequence typing system

KW - infectious-disease

KW - Jejuni infection

KW - public-health

KW - coli

KW - England

KW - poultry

KW - Wales

KW - food

KW - contamination

U2 - 10.1128/JCM.01731-08

DO - 10.1128/JCM.01731-08

M3 - Article

VL - 47

SP - 111

EP - 116

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -