Candida albicans Primes TLR Cytokine Responses through a Dectin-1/Raf-1–Mediated Pathway

Daniela C. Ifrim, Leo A. B. Joosten, Bart-Jan Kullberg, Liesbeth Jacobs, Trees Jansen, David L. Williams, Neil A. R. Gow, Jos W. M. Van Der Meer, Mihai G. Netea, Jessica Quintin*

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Citations (Scopus)
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Abstract

The immune system is essential to maintain homeostasis with resident microbial populations, ensuring that the symbiotic host-microbial relationship is maintained. In parallel, commensal microbes significantly shape mammalian immunity at the host mucosal surface, as well as systemically. Candida albicans is an opportunistic pathogen that lives as a commensal on skin and mucosa of healthy individuals. Little is known about its capacity to modulate responses toward other microorganisms, such as colonizing bacteria (e.g., intestinal microorganisms). The aim of this study was to assess the cytokine production of PBMCs induced by commensal bacteria when these cells were primed by C. albicans. We show that C. albicans and β-1,3-glucan induce priming of human primary mononuclear cells and this leads to enhanced cytokine production upon in vitro stimulation with TLR ligands and bacterial commensals. This priming requires the β-1,3-glucan receptor dectin-1 and the noncanonical Raf-1 pathway. In addition, although purified mannans cannot solely mediate the priming, the presence of mannosyl residues in the cell wall of C. albicans is nevertheless required. In conclusion, C. albicans is able to modify cytokine responses to TLR ligands and colonizing bacteria, which is likely to impact the inflammatory reaction during mucosal diseases.

Original languageEnglish
Pages (from-to)4129-4135
Number of pages7
JournalThe Journal of Immunology
Volume190
Issue number8
Early online date5 Apr 2013
DOIs
Publication statusPublished - 15 Apr 2013

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Candida albicans
Cytokines
Glucans
Bacteria
Ligands
Mannans
Cell Wall
Immune System
Immunity
Mucous Membrane
Homeostasis
dectin 1
Skin
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Ifrim, D. C., Joosten, L. A. B., Kullberg, B-J., Jacobs, L., Jansen, T., Williams, D. L., ... Quintin, J. (2013). Candida albicans Primes TLR Cytokine Responses through a Dectin-1/Raf-1–Mediated Pathway. The Journal of Immunology, 190(8), 4129-4135. https://doi.org/10.4049/jimmunol.1202611

Candida albicans Primes TLR Cytokine Responses through a Dectin-1/Raf-1–Mediated Pathway. / Ifrim, Daniela C.; Joosten, Leo A. B.; Kullberg, Bart-Jan; Jacobs, Liesbeth; Jansen, Trees; Williams, David L.; Gow, Neil A. R.; Van Der Meer, Jos W. M.; Netea, Mihai G.; Quintin, Jessica.

In: The Journal of Immunology, Vol. 190, No. 8, 15.04.2013, p. 4129-4135.

Research output: Contribution to journalArticle

Ifrim, DC, Joosten, LAB, Kullberg, B-J, Jacobs, L, Jansen, T, Williams, DL, Gow, NAR, Van Der Meer, JWM, Netea, MG & Quintin, J 2013, 'Candida albicans Primes TLR Cytokine Responses through a Dectin-1/Raf-1–Mediated Pathway', The Journal of Immunology, vol. 190, no. 8, pp. 4129-4135. https://doi.org/10.4049/jimmunol.1202611
Ifrim DC, Joosten LAB, Kullberg B-J, Jacobs L, Jansen T, Williams DL et al. Candida albicans Primes TLR Cytokine Responses through a Dectin-1/Raf-1–Mediated Pathway. The Journal of Immunology. 2013 Apr 15;190(8):4129-4135. https://doi.org/10.4049/jimmunol.1202611
Ifrim, Daniela C. ; Joosten, Leo A. B. ; Kullberg, Bart-Jan ; Jacobs, Liesbeth ; Jansen, Trees ; Williams, David L. ; Gow, Neil A. R. ; Van Der Meer, Jos W. M. ; Netea, Mihai G. ; Quintin, Jessica. / Candida albicans Primes TLR Cytokine Responses through a Dectin-1/Raf-1–Mediated Pathway. In: The Journal of Immunology. 2013 ; Vol. 190, No. 8. pp. 4129-4135.
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abstract = "The immune system is essential to maintain homeostasis with resident microbial populations, ensuring that the symbiotic host-microbial relationship is maintained. In parallel, commensal microbes significantly shape mammalian immunity at the host mucosal surface, as well as systemically. Candida albicans is an opportunistic pathogen that lives as a commensal on skin and mucosa of healthy individuals. Little is known about its capacity to modulate responses toward other microorganisms, such as colonizing bacteria (e.g., intestinal microorganisms). The aim of this study was to assess the cytokine production of PBMCs induced by commensal bacteria when these cells were primed by C. albicans. We show that C. albicans and β-1,3-glucan induce priming of human primary mononuclear cells and this leads to enhanced cytokine production upon in vitro stimulation with TLR ligands and bacterial commensals. This priming requires the β-1,3-glucan receptor dectin-1 and the noncanonical Raf-1 pathway. In addition, although purified mannans cannot solely mediate the priming, the presence of mannosyl residues in the cell wall of C. albicans is nevertheless required. In conclusion, C. albicans is able to modify cytokine responses to TLR ligands and colonizing bacteria, which is likely to impact the inflammatory reaction during mucosal diseases.",
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