Cellular location and some properties of proteolytic enzymes of rumen bacteria

J. Kopecny, R. J. Wallace

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Several physical and chemical techniques were used to extract, and to identify the location of, proteolytic enzymes associated with mixed rumen bacteria. Most activity was removable by gentle physical methods such as shaking and brief blending, without cell disruption, indicating that it was associated with coat and capsular material. Proteases were present also in the cell envelope, corresponding to the inner membrane fraction of gram-negative bacteria, and intracellularly and were removable by detergent and French press treatment. Temperature and pH profiles were obtained for the coat enzymes, likely to be the most important in the digestion of food protein. Inhibition studies indicated that these proteases, and those of the whole bacterial fraction from rumen fluid, were predominantly of the cysteine protease type.
Original languageEnglish
Pages (from-to)1026-1033
Number of pages8
JournalApplied and Environmental Microbiology
Volume43
Issue number5
Publication statusPublished - May 1982

Fingerprint

rumen bacteria
Rumen
Peptide Hydrolases
proteinases
enzyme
Bacteria
physical method
bacterium
cysteine proteinases
detergent
rumen fluids
enzymes
Gram-negative bacteria
detergents
protein sources
digestion
Cysteine Proteases
cells
membrane
Gram-Negative Bacteria

Cite this

Cellular location and some properties of proteolytic enzymes of rumen bacteria. / Kopecny, J.; Wallace, R. J.

In: Applied and Environmental Microbiology, Vol. 43, No. 5, 05.1982, p. 1026-1033.

Research output: Contribution to journalArticle

@article{88dc54d127c04068899fd789caa0bf23,
title = "Cellular location and some properties of proteolytic enzymes of rumen bacteria",
abstract = "Several physical and chemical techniques were used to extract, and to identify the location of, proteolytic enzymes associated with mixed rumen bacteria. Most activity was removable by gentle physical methods such as shaking and brief blending, without cell disruption, indicating that it was associated with coat and capsular material. Proteases were present also in the cell envelope, corresponding to the inner membrane fraction of gram-negative bacteria, and intracellularly and were removable by detergent and French press treatment. Temperature and pH profiles were obtained for the coat enzymes, likely to be the most important in the digestion of food protein. Inhibition studies indicated that these proteases, and those of the whole bacterial fraction from rumen fluid, were predominantly of the cysteine protease type.",
author = "J. Kopecny and Wallace, {R. J.}",
note = "Copyright 2004 Elsevier B.V., All rights reserved.",
year = "1982",
month = "5",
language = "English",
volume = "43",
pages = "1026--1033",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Cellular location and some properties of proteolytic enzymes of rumen bacteria

AU - Kopecny, J.

AU - Wallace, R. J.

N1 - Copyright 2004 Elsevier B.V., All rights reserved.

PY - 1982/5

Y1 - 1982/5

N2 - Several physical and chemical techniques were used to extract, and to identify the location of, proteolytic enzymes associated with mixed rumen bacteria. Most activity was removable by gentle physical methods such as shaking and brief blending, without cell disruption, indicating that it was associated with coat and capsular material. Proteases were present also in the cell envelope, corresponding to the inner membrane fraction of gram-negative bacteria, and intracellularly and were removable by detergent and French press treatment. Temperature and pH profiles were obtained for the coat enzymes, likely to be the most important in the digestion of food protein. Inhibition studies indicated that these proteases, and those of the whole bacterial fraction from rumen fluid, were predominantly of the cysteine protease type.

AB - Several physical and chemical techniques were used to extract, and to identify the location of, proteolytic enzymes associated with mixed rumen bacteria. Most activity was removable by gentle physical methods such as shaking and brief blending, without cell disruption, indicating that it was associated with coat and capsular material. Proteases were present also in the cell envelope, corresponding to the inner membrane fraction of gram-negative bacteria, and intracellularly and were removable by detergent and French press treatment. Temperature and pH profiles were obtained for the coat enzymes, likely to be the most important in the digestion of food protein. Inhibition studies indicated that these proteases, and those of the whole bacterial fraction from rumen fluid, were predominantly of the cysteine protease type.

UR - http://www.scopus.com/inward/record.url?scp=0019952778&partnerID=8YFLogxK

M3 - Article

VL - 43

SP - 1026

EP - 1033

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 5

ER -