Characterisation and expression analysis of the rainbow trout (Oncorhynchus mykiss) homologue of the human dendritic cell marker CD208/lysosomal associated membrane protein 3

Petronella Johansson, Yolanda Corripio-Miyar, Tiehui Wang, Bertrand Collet, Chris J Secombes, Jun Zou

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

LAMP3/CD208 is a member of the lysosomal-associated membrane protein family and is used as a putative marker for mature dendritic cells (DCs) in humans since it is upregulated upon DC activation and maturation. This paper reports the cloning and sequencing of LAMP3 in rainbow trout. The predicted trout LAMP3 shares the characteristic features of LAMP family members such as a C-terminal lysosomal sorting motif (G-Y-D-R-I) in the short C-terminal cytoplasmic tail, typical for lysosomal targeting, four potential N-linked glycosylation sites (NXS/T), four conserved cysteines in the membrane-proximal domain and the luminal domain divided by a serine/proline-rich region. Expression studies revealed that trout LAMP3, like chicken LAMP3, was constitutively expressed in a wide range of lymphoid tissues, at highest levels in the head kidney, liver and spleen, respectively. LAMP3 was also constitutively expressed in trout head kidney macrophages and RTS11 cells and the expression was shown to be induced in vivo after infection with viral and bacterial pathogens and in cultured macrophages after modulation with microbial mimics (LPS and PolyIC). Thus, it is clear that if LAMP3 is expressed by trout DCs it is not exclusively expressed by them. However, the marker is valuable to further study antigen presentation in fish and to complement already known DC markers.
Original languageEnglish
Pages (from-to)402-413
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume37
Issue number3-4
Early online date6 Mar 2012
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Lysosomal-Associated Membrane Protein 3
Trout
Oncorhynchus mykiss
Dendritic Cells
Head Kidney
Macrophages
Lysosome-Associated Membrane Glycoproteins
Antigen Presentation
Lymphoid Tissue
Virus Diseases
Glycosylation
Bacterial Infections
Proline
Serine
Cysteine
Tail
Organism Cloning
Chickens
Fishes
Spleen

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Dendritic Cells
  • Fish Proteins
  • Genes, MHC Class II
  • Head Kidney
  • Humans
  • Lipopolysaccharides
  • Lysosomal-Associated Membrane Protein 3
  • Lysosome-Associated Membrane Glycoproteins
  • Macrophages
  • Molecular Sequence Data
  • Neoplasm Proteins
  • Oncorhynchus mykiss
  • Phylogeny
  • Poly I-C
  • Sequence Alignment
  • Rainbow Trout
  • LAMP3/CD208
  • Maturation Markers

Cite this

Characterisation and expression analysis of the rainbow trout (Oncorhynchus mykiss) homologue of the human dendritic cell marker CD208/lysosomal associated membrane protein 3. / Johansson, Petronella; Corripio-Miyar, Yolanda; Wang, Tiehui; Collet, Bertrand; Secombes, Chris J; Zou, Jun.

In: Developmental and Comparative Immunology, Vol. 37, No. 3-4, 07.2012, p. 402-413.

Research output: Contribution to journalArticle

@article{b066c39e63ba4defaebd25bbce279bad,
title = "Characterisation and expression analysis of the rainbow trout (Oncorhynchus mykiss) homologue of the human dendritic cell marker CD208/lysosomal associated membrane protein 3",
abstract = "LAMP3/CD208 is a member of the lysosomal-associated membrane protein family and is used as a putative marker for mature dendritic cells (DCs) in humans since it is upregulated upon DC activation and maturation. This paper reports the cloning and sequencing of LAMP3 in rainbow trout. The predicted trout LAMP3 shares the characteristic features of LAMP family members such as a C-terminal lysosomal sorting motif (G-Y-D-R-I) in the short C-terminal cytoplasmic tail, typical for lysosomal targeting, four potential N-linked glycosylation sites (NXS/T), four conserved cysteines in the membrane-proximal domain and the luminal domain divided by a serine/proline-rich region. Expression studies revealed that trout LAMP3, like chicken LAMP3, was constitutively expressed in a wide range of lymphoid tissues, at highest levels in the head kidney, liver and spleen, respectively. LAMP3 was also constitutively expressed in trout head kidney macrophages and RTS11 cells and the expression was shown to be induced in vivo after infection with viral and bacterial pathogens and in cultured macrophages after modulation with microbial mimics (LPS and PolyIC). Thus, it is clear that if LAMP3 is expressed by trout DCs it is not exclusively expressed by them. However, the marker is valuable to further study antigen presentation in fish and to complement already known DC markers.",
keywords = "Amino Acid Sequence, Animals, Base Sequence, Cell Line, Dendritic Cells, Fish Proteins, Genes, MHC Class II, Head Kidney, Humans, Lipopolysaccharides, Lysosomal-Associated Membrane Protein 3, Lysosome-Associated Membrane Glycoproteins, Macrophages, Molecular Sequence Data, Neoplasm Proteins, Oncorhynchus mykiss, Phylogeny, Poly I-C, Sequence Alignment, Rainbow Trout, LAMP3/CD208, Maturation Markers",
author = "Petronella Johansson and Yolanda Corripio-Miyar and Tiehui Wang and Bertrand Collet and Secombes, {Chris J} and Jun Zou",
note = "Copyright {\circledC} 2012 Elsevier Ltd. All rights reserved.",
year = "2012",
month = "7",
doi = "10.1016/j.dci.2012.02.012",
language = "English",
volume = "37",
pages = "402--413",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",
number = "3-4",

}

TY - JOUR

T1 - Characterisation and expression analysis of the rainbow trout (Oncorhynchus mykiss) homologue of the human dendritic cell marker CD208/lysosomal associated membrane protein 3

AU - Johansson, Petronella

AU - Corripio-Miyar, Yolanda

AU - Wang, Tiehui

AU - Collet, Bertrand

AU - Secombes, Chris J

AU - Zou, Jun

N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.

PY - 2012/7

Y1 - 2012/7

N2 - LAMP3/CD208 is a member of the lysosomal-associated membrane protein family and is used as a putative marker for mature dendritic cells (DCs) in humans since it is upregulated upon DC activation and maturation. This paper reports the cloning and sequencing of LAMP3 in rainbow trout. The predicted trout LAMP3 shares the characteristic features of LAMP family members such as a C-terminal lysosomal sorting motif (G-Y-D-R-I) in the short C-terminal cytoplasmic tail, typical for lysosomal targeting, four potential N-linked glycosylation sites (NXS/T), four conserved cysteines in the membrane-proximal domain and the luminal domain divided by a serine/proline-rich region. Expression studies revealed that trout LAMP3, like chicken LAMP3, was constitutively expressed in a wide range of lymphoid tissues, at highest levels in the head kidney, liver and spleen, respectively. LAMP3 was also constitutively expressed in trout head kidney macrophages and RTS11 cells and the expression was shown to be induced in vivo after infection with viral and bacterial pathogens and in cultured macrophages after modulation with microbial mimics (LPS and PolyIC). Thus, it is clear that if LAMP3 is expressed by trout DCs it is not exclusively expressed by them. However, the marker is valuable to further study antigen presentation in fish and to complement already known DC markers.

AB - LAMP3/CD208 is a member of the lysosomal-associated membrane protein family and is used as a putative marker for mature dendritic cells (DCs) in humans since it is upregulated upon DC activation and maturation. This paper reports the cloning and sequencing of LAMP3 in rainbow trout. The predicted trout LAMP3 shares the characteristic features of LAMP family members such as a C-terminal lysosomal sorting motif (G-Y-D-R-I) in the short C-terminal cytoplasmic tail, typical for lysosomal targeting, four potential N-linked glycosylation sites (NXS/T), four conserved cysteines in the membrane-proximal domain and the luminal domain divided by a serine/proline-rich region. Expression studies revealed that trout LAMP3, like chicken LAMP3, was constitutively expressed in a wide range of lymphoid tissues, at highest levels in the head kidney, liver and spleen, respectively. LAMP3 was also constitutively expressed in trout head kidney macrophages and RTS11 cells and the expression was shown to be induced in vivo after infection with viral and bacterial pathogens and in cultured macrophages after modulation with microbial mimics (LPS and PolyIC). Thus, it is clear that if LAMP3 is expressed by trout DCs it is not exclusively expressed by them. However, the marker is valuable to further study antigen presentation in fish and to complement already known DC markers.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Cell Line

KW - Dendritic Cells

KW - Fish Proteins

KW - Genes, MHC Class II

KW - Head Kidney

KW - Humans

KW - Lipopolysaccharides

KW - Lysosomal-Associated Membrane Protein 3

KW - Lysosome-Associated Membrane Glycoproteins

KW - Macrophages

KW - Molecular Sequence Data

KW - Neoplasm Proteins

KW - Oncorhynchus mykiss

KW - Phylogeny

KW - Poly I-C

KW - Sequence Alignment

KW - Rainbow Trout

KW - LAMP3/CD208

KW - Maturation Markers

U2 - 10.1016/j.dci.2012.02.012

DO - 10.1016/j.dci.2012.02.012

M3 - Article

VL - 37

SP - 402

EP - 413

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 3-4

ER -