Characterisation of and changes to pro and anti-oxidant enzyme activities during the hypersensitive reaction in lettuce (Lactuca sativa L.)

Charles Bestwick, A L Adam, N Puri, J W Mansfield

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Enzymes regulating aspects of reactive oxygen metabolism were characterised in expanded lettuce leaf tissue. Catalase (CAT) activity was optimal between pH 7.0-8.0. predominantly located within peroxisomes and comprised two isoenzymes (pI 5.8 and 6.2). Of three principal superoxide dismutase (SOD) isoenzymes detected., CuZn SOD, (pI 4.90) represented 51.7%, Mn SOD (pI 4.75) 14.6% and a putative Fe SOD (pI 4.85), 33.7% of total activity. Lipoxygenase (LOX) activity was optimal at pH 5. Activities were assessed following inoculation with Psendomonas syringae pv. phaseolicola and a related hrpD mutant. The wild-type bacterium induced a rapid hypersensitive reaction (HR), associated with a progressive increase in thiobarbituric acid reactive substances (TBARS), indicative of lipid peroxidation, a sustained increase in LOX activity and a later increase in CAT activity. The hrp mutant induced a transitory increase in TBARS. a transient increase in LOX activity, again a later elevation in CAT activity but no macroscopic tissue collapse. In comparison. water infiltration induced a rapid but transient increase in both SOD and CAT activities. From this and our previous work, it is concluded that lettuce cells undergoing an HR experience a prolonged oxidative stress. primarily through an increase in pro-oxidant activities initially occurring in the absence of enhanced antioxidant activities. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)497-506
Number of pages10
JournalPlant Science
Volume161
Issue number3
DOIs
Publication statusPublished - Aug 2001

Keywords

  • antioxidants
  • hypersensitive reactions
  • Lactuca sativa L
  • lipoxygenase
  • oxidative stress
  • Pseudomonas syringae pv. phaseolicola
  • syringae PV-phaseolicola
  • programmed cell-death
  • oxidative burst
  • hydrogen-peroxide
  • superoxide-dismutase
  • membrane damage
  • bremia-lactuae
  • accumulation
  • oxygen

Cite this

Characterisation of and changes to pro and anti-oxidant enzyme activities during the hypersensitive reaction in lettuce (Lactuca sativa L.). / Bestwick, Charles; Adam, A L ; Puri, N ; Mansfield, J W .

In: Plant Science, Vol. 161, No. 3, 08.2001, p. 497-506.

Research output: Contribution to journalArticle

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AB - Enzymes regulating aspects of reactive oxygen metabolism were characterised in expanded lettuce leaf tissue. Catalase (CAT) activity was optimal between pH 7.0-8.0. predominantly located within peroxisomes and comprised two isoenzymes (pI 5.8 and 6.2). Of three principal superoxide dismutase (SOD) isoenzymes detected., CuZn SOD, (pI 4.90) represented 51.7%, Mn SOD (pI 4.75) 14.6% and a putative Fe SOD (pI 4.85), 33.7% of total activity. Lipoxygenase (LOX) activity was optimal at pH 5. Activities were assessed following inoculation with Psendomonas syringae pv. phaseolicola and a related hrpD mutant. The wild-type bacterium induced a rapid hypersensitive reaction (HR), associated with a progressive increase in thiobarbituric acid reactive substances (TBARS), indicative of lipid peroxidation, a sustained increase in LOX activity and a later increase in CAT activity. The hrp mutant induced a transitory increase in TBARS. a transient increase in LOX activity, again a later elevation in CAT activity but no macroscopic tissue collapse. In comparison. water infiltration induced a rapid but transient increase in both SOD and CAT activities. From this and our previous work, it is concluded that lettuce cells undergoing an HR experience a prolonged oxidative stress. primarily through an increase in pro-oxidant activities initially occurring in the absence of enhanced antioxidant activities. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

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KW - hypersensitive reactions

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KW - oxidative stress

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KW - programmed cell-death

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KW - hydrogen-peroxide

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KW - accumulation

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