Abstract
Enzymes regulating aspects of reactive oxygen metabolism were characterised in expanded lettuce leaf tissue. Catalase (CAT) activity was optimal between pH 7.0-8.0. predominantly located within peroxisomes and comprised two isoenzymes (pI 5.8 and 6.2). Of three principal superoxide dismutase (SOD) isoenzymes detected., CuZn SOD, (pI 4.90) represented 51.7%, Mn SOD (pI 4.75) 14.6% and a putative Fe SOD (pI 4.85), 33.7% of total activity. Lipoxygenase (LOX) activity was optimal at pH 5. Activities were assessed following inoculation with Psendomonas syringae pv. phaseolicola and a related hrpD mutant. The wild-type bacterium induced a rapid hypersensitive reaction (HR), associated with a progressive increase in thiobarbituric acid reactive substances (TBARS), indicative of lipid peroxidation, a sustained increase in LOX activity and a later increase in CAT activity. The hrp mutant induced a transitory increase in TBARS. a transient increase in LOX activity, again a later elevation in CAT activity but no macroscopic tissue collapse. In comparison. water infiltration induced a rapid but transient increase in both SOD and CAT activities. From this and our previous work, it is concluded that lettuce cells undergoing an HR experience a prolonged oxidative stress. primarily through an increase in pro-oxidant activities initially occurring in the absence of enhanced antioxidant activities. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
Original language | English |
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Pages (from-to) | 497-506 |
Number of pages | 10 |
Journal | Plant Science |
Volume | 161 |
Issue number | 3 |
DOIs | |
Publication status | Published - Aug 2001 |
Keywords
- antioxidants
- hypersensitive reactions
- Lactuca sativa L
- lipoxygenase
- oxidative stress
- Pseudomonas syringae pv. phaseolicola
- syringae PV-phaseolicola
- programmed cell-death
- oxidative burst
- hydrogen-peroxide
- superoxide-dismutase
- membrane damage
- bremia-lactuae
- accumulation
- oxygen