Characterisation of hoxa gene expression in the chick limb bud in response to FGF

Neil Vargesson, K Kostakopoulou, G Drossopoulou, S Papageorgiou, C Tickle

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We tested a diffusion gradient model for setting up overlapping domains of Hoxa gene expression in the chick limb bud. The model is based on morphogen production at the limb bud tip where the apical ridge is located and assumes that cells respond to a series of concentration thresholds. Consistent with the model, Hoxa13 gene expression rapidly switches off when the ridge is removed from stage 21/22 buds, while Hoxa11 and Hoxa10 expression is stable; Hoxa13 expression can be initiated and maintained in absence of the ridge by FGF soaked beads; the Hoxa13 domain first expands quickly and then slows up and the size is related to the dose of FGF4. Contrary to the model, addition of FGF4 to early limb buds does not activate Hoxa13 prematurely nor extend the Hoxa13 expression domain proximally. Therefore FGF4 signalling is necessary but not sufficient for Hoxa gene expression in the limb bud.
Original languageEnglish
Pages (from-to)87-90
Number of pages4
JournalDevelopmental Dynamics
Volume220
Issue number1
Early online date16 Nov 2000
DOIs
Publication statusPublished - 1 Jan 2001

Keywords

  • Animals
  • Chick Embryo
  • DNA-Binding Proteins
  • Fibroblast Growth Factor 4
  • Fibroblast Growth Factors
  • Homeodomain Proteins
  • In Situ Hybridization
  • Limb Buds
  • Proto-Oncogene Proteins
  • Time Factors
  • Trans-Activators
  • chick embryo
  • limb development
  • Hox gene
  • FGF

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